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Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults

The purpose of this study was to analyze corneal sub-basal nerve plexus (SBNP) density and morphology and their relationships with corneal parameters and refractive status. In this single center study, in vivo confocal microscopy (IVCM) was performed in 76 eyes of 38 healthy subjects aged 19–87 (mea...

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Autores principales: Roszkowska, Anna M., Wylęgała, Adam, Gargano, Romana, Spinella, Rosaria, Inferrera, Leandro, Orzechowska-Wylęgała, Bogusława, Aragona, Pasquale
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966734/
https://www.ncbi.nlm.nih.gov/pubmed/33727601
http://dx.doi.org/10.1038/s41598-021-85597-5
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author Roszkowska, Anna M.
Wylęgała, Adam
Gargano, Romana
Spinella, Rosaria
Inferrera, Leandro
Orzechowska-Wylęgała, Bogusława
Aragona, Pasquale
author_facet Roszkowska, Anna M.
Wylęgała, Adam
Gargano, Romana
Spinella, Rosaria
Inferrera, Leandro
Orzechowska-Wylęgała, Bogusława
Aragona, Pasquale
author_sort Roszkowska, Anna M.
collection PubMed
description The purpose of this study was to analyze corneal sub-basal nerve plexus (SBNP) density and morphology and their relationships with corneal parameters and refractive status. In this single center study, in vivo confocal microscopy (IVCM) was performed in 76 eyes of 38 healthy subjects aged 19–87 (mean age 34.987 ± 1.148). Nerve fiber analysis was performed using Confoscan 4 microscope with semi-automated software (Nidek Technologies, Italy) The nerve fiber length (NFL) µm/mm(2), nerve fiber density (NFD) no./mm(2), tortuosity coefficient (TC), and nerve beadings density (NBD) no./mm were considered. Relationship between SBNP parameters and corneal curvature, thickness, diameter, and refraction were analyzed. Additionally, the association with gender, laterality and age were determined. NFL was inversely correlated with age (r = − 0.528, p < 0.001), myopic refractive error (spherical value) (r = − 0.423, p < 0.001), and cylindrical power (r = − 0.340, p = 0.003). NFD was inversely correlated with age (r = − 0.420, p < 0.001) and myopic refractive error (r = − 0.341, p = 0.003). NBD showed a low inverse correlation with cylindrical power (r = − 0.287, p = 0.012) and a slight positive correlation with K (r = 0.230, p = 0.047). TC showed a significant negative correlation between age (r = − 0.500, p < 0.001) and myopic refractive error (r = − 0.351, p = 0.002). Additionally, there were strong positive correlations between NFL and NFD (r = 0.523, p < 0.001), NFL and TI (r = 0.603, p < 0.001), and NFD and TC (r = 0.758, p < 0.001). Multiple regression analysis revealed age to be the most significant factor affecting SBNP density (B = − 0.467, p = 0.013) and length (B = − 61.446, p < 0.001); myopic refractive error reduced both SBNP density (B = − 2.119, p = 0.011) and length (B = − 158.433, p = 0.016), while gender and laterality had no significant effects (p > 0.005). SBNP fiber length decreases with age, myopic refractive error and cylindrical power. SBNP fiber density reduces with age and myopic refractive error. Corneal nerve parameters are not influenced by gender or laterality.
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spelling pubmed-79667342021-03-19 Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults Roszkowska, Anna M. Wylęgała, Adam Gargano, Romana Spinella, Rosaria Inferrera, Leandro Orzechowska-Wylęgała, Bogusława Aragona, Pasquale Sci Rep Article The purpose of this study was to analyze corneal sub-basal nerve plexus (SBNP) density and morphology and their relationships with corneal parameters and refractive status. In this single center study, in vivo confocal microscopy (IVCM) was performed in 76 eyes of 38 healthy subjects aged 19–87 (mean age 34.987 ± 1.148). Nerve fiber analysis was performed using Confoscan 4 microscope with semi-automated software (Nidek Technologies, Italy) The nerve fiber length (NFL) µm/mm(2), nerve fiber density (NFD) no./mm(2), tortuosity coefficient (TC), and nerve beadings density (NBD) no./mm were considered. Relationship between SBNP parameters and corneal curvature, thickness, diameter, and refraction were analyzed. Additionally, the association with gender, laterality and age were determined. NFL was inversely correlated with age (r = − 0.528, p < 0.001), myopic refractive error (spherical value) (r = − 0.423, p < 0.001), and cylindrical power (r = − 0.340, p = 0.003). NFD was inversely correlated with age (r = − 0.420, p < 0.001) and myopic refractive error (r = − 0.341, p = 0.003). NBD showed a low inverse correlation with cylindrical power (r = − 0.287, p = 0.012) and a slight positive correlation with K (r = 0.230, p = 0.047). TC showed a significant negative correlation between age (r = − 0.500, p < 0.001) and myopic refractive error (r = − 0.351, p = 0.002). Additionally, there were strong positive correlations between NFL and NFD (r = 0.523, p < 0.001), NFL and TI (r = 0.603, p < 0.001), and NFD and TC (r = 0.758, p < 0.001). Multiple regression analysis revealed age to be the most significant factor affecting SBNP density (B = − 0.467, p = 0.013) and length (B = − 61.446, p < 0.001); myopic refractive error reduced both SBNP density (B = − 2.119, p = 0.011) and length (B = − 158.433, p = 0.016), while gender and laterality had no significant effects (p > 0.005). SBNP fiber length decreases with age, myopic refractive error and cylindrical power. SBNP fiber density reduces with age and myopic refractive error. Corneal nerve parameters are not influenced by gender or laterality. Nature Publishing Group UK 2021-03-16 /pmc/articles/PMC7966734/ /pubmed/33727601 http://dx.doi.org/10.1038/s41598-021-85597-5 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Roszkowska, Anna M.
Wylęgała, Adam
Gargano, Romana
Spinella, Rosaria
Inferrera, Leandro
Orzechowska-Wylęgała, Bogusława
Aragona, Pasquale
Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults
title Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults
title_full Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults
title_fullStr Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults
title_full_unstemmed Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults
title_short Impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults
title_sort impact of corneal parameters, refractive error and age on density and morphology of the subbasal nerve plexus fibers in healthy adults
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966734/
https://www.ncbi.nlm.nih.gov/pubmed/33727601
http://dx.doi.org/10.1038/s41598-021-85597-5
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