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Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils

The environment of plant rhizosphere soil differs from that of non-rhizosphere soil due to the secretion of mucilage polysaccharides from the roots. This environment is regarded as one of the preferential habitats for agar-degrading bacteria. In a previous study, agar-degrading Steroidobacter agarip...

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Autores principales: Ikenaga, Makoto, Kataoka, Machi, Yin, Xuan, Murouchi, Aya, Sakai, Masao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966939/
https://www.ncbi.nlm.nih.gov/pubmed/33716238
http://dx.doi.org/10.1264/jsme2.ME20136
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author Ikenaga, Makoto
Kataoka, Machi
Yin, Xuan
Murouchi, Aya
Sakai, Masao
author_facet Ikenaga, Makoto
Kataoka, Machi
Yin, Xuan
Murouchi, Aya
Sakai, Masao
author_sort Ikenaga, Makoto
collection PubMed
description The environment of plant rhizosphere soil differs from that of non-rhizosphere soil due to the secretion of mucilage polysaccharides from the roots. This environment is regarded as one of the preferential habitats for agar-degrading bacteria. In a previous study, agar-degrading Steroidobacter agariperforans KA5-B(T) was isolated from agar-enriched agricultural soil using diffusible metabolites from Rhizobiales bacteria. Based on the hypothesis that similar characteristic bacteria still exist in the rhizosphere, isolation was performed using rhizosphere soils. Agar-degrading SA29-B(T) and YU21-B were isolated from onion and soybean rhizosphere soils. The 16S rRNA genes of these strains showed ≥98.7% identities with the most closely related strain KA5-B(T). However, differences were noted in polysaccharide utilization, and average nucleotide identities were <95–96% against strain KA5-B(T), indicating that they are different species from S. agariperforans KA5-B(T). To investigate the distribution of bacterial sequences affiliated with novel strains, a primer set was designed and a meta-analysis of the 16S rRNA gene was performed. Sequences were widely distributed in rhizospheres throughout Japan, but varied in plant- and region-dependent manners. Regarding phenotypic characterization, distinguishable features were observed in growth temperatures, pH, and dominant fatty acids. SA29-B(T) and YU21-B grew at 15–40°C and pH 6.0–12 and contained C(16:0) as the dominant cell fatty acid, whereas KA5-B(T) showed no growth at 40°C and pH 12 and contained a moderate amount of C(16:0). Based on these characteristics, SA29-B(T) (JCM 333368(T)=KCTC 72223(T)) and YU21-B (JCM 333367=KCTC 72222) represent novel species in the genus Steroidobacter, for which the name Steroidobacter agaridevorans sp. nov. is proposed.
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spelling pubmed-79669392021-03-26 Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils Ikenaga, Makoto Kataoka, Machi Yin, Xuan Murouchi, Aya Sakai, Masao Microbes Environ Regular Paper The environment of plant rhizosphere soil differs from that of non-rhizosphere soil due to the secretion of mucilage polysaccharides from the roots. This environment is regarded as one of the preferential habitats for agar-degrading bacteria. In a previous study, agar-degrading Steroidobacter agariperforans KA5-B(T) was isolated from agar-enriched agricultural soil using diffusible metabolites from Rhizobiales bacteria. Based on the hypothesis that similar characteristic bacteria still exist in the rhizosphere, isolation was performed using rhizosphere soils. Agar-degrading SA29-B(T) and YU21-B were isolated from onion and soybean rhizosphere soils. The 16S rRNA genes of these strains showed ≥98.7% identities with the most closely related strain KA5-B(T). However, differences were noted in polysaccharide utilization, and average nucleotide identities were <95–96% against strain KA5-B(T), indicating that they are different species from S. agariperforans KA5-B(T). To investigate the distribution of bacterial sequences affiliated with novel strains, a primer set was designed and a meta-analysis of the 16S rRNA gene was performed. Sequences were widely distributed in rhizospheres throughout Japan, but varied in plant- and region-dependent manners. Regarding phenotypic characterization, distinguishable features were observed in growth temperatures, pH, and dominant fatty acids. SA29-B(T) and YU21-B grew at 15–40°C and pH 6.0–12 and contained C(16:0) as the dominant cell fatty acid, whereas KA5-B(T) showed no growth at 40°C and pH 12 and contained a moderate amount of C(16:0). Based on these characteristics, SA29-B(T) (JCM 333368(T)=KCTC 72223(T)) and YU21-B (JCM 333367=KCTC 72222) represent novel species in the genus Steroidobacter, for which the name Steroidobacter agaridevorans sp. nov. is proposed. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2021 2021-03-13 /pmc/articles/PMC7966939/ /pubmed/33716238 http://dx.doi.org/10.1264/jsme2.ME20136 Text en 2021 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Regular Paper
Ikenaga, Makoto
Kataoka, Machi
Yin, Xuan
Murouchi, Aya
Sakai, Masao
Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils
title Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils
title_full Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils
title_fullStr Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils
title_full_unstemmed Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils
title_short Characterization and Distribution of Agar-degrading Steroidobacter agaridevorans sp. nov., Isolated from Rhizosphere Soils
title_sort characterization and distribution of agar-degrading steroidobacter agaridevorans sp. nov., isolated from rhizosphere soils
topic Regular Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7966939/
https://www.ncbi.nlm.nih.gov/pubmed/33716238
http://dx.doi.org/10.1264/jsme2.ME20136
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