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Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols
Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans. In the present study, the effect of polyphenols gallic acid a...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967010/ https://www.ncbi.nlm.nih.gov/pubmed/33748439 http://dx.doi.org/10.1016/j.bbrep.2021.100980 |
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author | Goyal, Dimple Ahmad, Shabeer Mahmood, Akhtar Chander Sharma, Sukesh |
author_facet | Goyal, Dimple Ahmad, Shabeer Mahmood, Akhtar Chander Sharma, Sukesh |
author_sort | Goyal, Dimple |
collection | PubMed |
description | Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans. In the present study, the effect of polyphenols gallic acid and tannic acid was investigated on the dextransucrase activity. The enzyme was purified by ethanol precipitation followed by column chromatography by Sephadex G-200 gel chromatography, followed by PEG-400 treatment. The purified enzyme exhibited 52 fold enrichment with 17.5% yield and specific activity of 3.54 Units/mg protein. On SDS-PAGE enzyme protein gave a single band with a molecular weight of 160 kDa. Dextransucrase activity was inhibited 80–90% by 0.04 mM tannic acid (TA) or 0.4 mM gallic acid (GA) suggesting that tannic acid has 10- fold more inhibitory potential than gallic acid on the activity of dextransucrase. CD/ORD studies revealed modifications in the tertiary structure of enzyme protein in presence of tannic acid and gallic acid, which were further confirmed by fluorescence spectra of the protein in presence of tannic acid. These results suggest that inhibition of dextransucrase activity in S. mutans by polyphenols may have potential applications in the prevention and control of dental caries. |
format | Online Article Text |
id | pubmed-7967010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-79670102021-03-19 Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols Goyal, Dimple Ahmad, Shabeer Mahmood, Akhtar Chander Sharma, Sukesh Biochem Biophys Rep Research Article Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans. In the present study, the effect of polyphenols gallic acid and tannic acid was investigated on the dextransucrase activity. The enzyme was purified by ethanol precipitation followed by column chromatography by Sephadex G-200 gel chromatography, followed by PEG-400 treatment. The purified enzyme exhibited 52 fold enrichment with 17.5% yield and specific activity of 3.54 Units/mg protein. On SDS-PAGE enzyme protein gave a single band with a molecular weight of 160 kDa. Dextransucrase activity was inhibited 80–90% by 0.04 mM tannic acid (TA) or 0.4 mM gallic acid (GA) suggesting that tannic acid has 10- fold more inhibitory potential than gallic acid on the activity of dextransucrase. CD/ORD studies revealed modifications in the tertiary structure of enzyme protein in presence of tannic acid and gallic acid, which were further confirmed by fluorescence spectra of the protein in presence of tannic acid. These results suggest that inhibition of dextransucrase activity in S. mutans by polyphenols may have potential applications in the prevention and control of dental caries. Elsevier 2021-03-11 /pmc/articles/PMC7967010/ /pubmed/33748439 http://dx.doi.org/10.1016/j.bbrep.2021.100980 Text en © 2021 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Goyal, Dimple Ahmad, Shabeer Mahmood, Akhtar Chander Sharma, Sukesh Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols |
title | Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols |
title_full | Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols |
title_fullStr | Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols |
title_full_unstemmed | Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols |
title_short | Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols |
title_sort | interactions of dextransucrase purified from streptococcus mutans 890 with plant polyphenols |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967010/ https://www.ncbi.nlm.nih.gov/pubmed/33748439 http://dx.doi.org/10.1016/j.bbrep.2021.100980 |
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