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Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols

Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans. In the present study, the effect of polyphenols gallic acid a...

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Autores principales: Goyal, Dimple, Ahmad, Shabeer, Mahmood, Akhtar, Chander Sharma, Sukesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967010/
https://www.ncbi.nlm.nih.gov/pubmed/33748439
http://dx.doi.org/10.1016/j.bbrep.2021.100980
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author Goyal, Dimple
Ahmad, Shabeer
Mahmood, Akhtar
Chander Sharma, Sukesh
author_facet Goyal, Dimple
Ahmad, Shabeer
Mahmood, Akhtar
Chander Sharma, Sukesh
author_sort Goyal, Dimple
collection PubMed
description Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans. In the present study, the effect of polyphenols gallic acid and tannic acid was investigated on the dextransucrase activity. The enzyme was purified by ethanol precipitation followed by column chromatography by Sephadex G-200 gel chromatography, followed by PEG-400 treatment. The purified enzyme exhibited 52 fold enrichment with 17.5% yield and specific activity of 3.54 Units/mg protein. On SDS-PAGE enzyme protein gave a single band with a molecular weight of 160 kDa. Dextransucrase activity was inhibited 80–90% by 0.04 mM tannic acid (TA) or 0.4 mM gallic acid (GA) suggesting that tannic acid has 10- fold more inhibitory potential than gallic acid on the activity of dextransucrase. CD/ORD studies revealed modifications in the tertiary structure of enzyme protein in presence of tannic acid and gallic acid, which were further confirmed by fluorescence spectra of the protein in presence of tannic acid. These results suggest that inhibition of dextransucrase activity in S. mutans by polyphenols may have potential applications in the prevention and control of dental caries.
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spelling pubmed-79670102021-03-19 Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols Goyal, Dimple Ahmad, Shabeer Mahmood, Akhtar Chander Sharma, Sukesh Biochem Biophys Rep Research Article Plant polyphenols have been extensively studied for their chemopreventive properties for human health. Dextransucrase plays an essential role in synthesizing exopolysaccharides from its exclusive substrate sucrose in Streptococcus mutans. In the present study, the effect of polyphenols gallic acid and tannic acid was investigated on the dextransucrase activity. The enzyme was purified by ethanol precipitation followed by column chromatography by Sephadex G-200 gel chromatography, followed by PEG-400 treatment. The purified enzyme exhibited 52 fold enrichment with 17.5% yield and specific activity of 3.54 Units/mg protein. On SDS-PAGE enzyme protein gave a single band with a molecular weight of 160 kDa. Dextransucrase activity was inhibited 80–90% by 0.04 mM tannic acid (TA) or 0.4 mM gallic acid (GA) suggesting that tannic acid has 10- fold more inhibitory potential than gallic acid on the activity of dextransucrase. CD/ORD studies revealed modifications in the tertiary structure of enzyme protein in presence of tannic acid and gallic acid, which were further confirmed by fluorescence spectra of the protein in presence of tannic acid. These results suggest that inhibition of dextransucrase activity in S. mutans by polyphenols may have potential applications in the prevention and control of dental caries. Elsevier 2021-03-11 /pmc/articles/PMC7967010/ /pubmed/33748439 http://dx.doi.org/10.1016/j.bbrep.2021.100980 Text en © 2021 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Goyal, Dimple
Ahmad, Shabeer
Mahmood, Akhtar
Chander Sharma, Sukesh
Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols
title Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols
title_full Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols
title_fullStr Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols
title_full_unstemmed Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols
title_short Interactions of dextransucrase purified from Streptococcus mutans 890 with plant polyphenols
title_sort interactions of dextransucrase purified from streptococcus mutans 890 with plant polyphenols
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967010/
https://www.ncbi.nlm.nih.gov/pubmed/33748439
http://dx.doi.org/10.1016/j.bbrep.2021.100980
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