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Hairpin‐Spacer crRNA‐Enhanced CRISPR/Cas13a System Promotes the Specificity of Single Nucleotide Polymorphism (SNP) Identification

The Cas13a system has great potential in RNA interference and molecular diagnostic fields. However, lacking guidelines for crRNA design hinders practical applications of the Cas13a system in RNA editing and single nucleotide polymorphism identification. This study posits that crRNAs with hairpin spa...

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Detalles Bibliográficos
Autores principales: Ke, Yuqing, Huang, Shiyi, Ghalandari, Behafarid, Li, Sijie, Warden, Antony R., Dang, Jingqi, Kang, Lin, Zhang, Yu, Wang, Yunqing, Sun, Yiqing, Wang, Jinglin, Cui, Daxiang, Zhi, Xiao, Ding, Xianting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967054/
https://www.ncbi.nlm.nih.gov/pubmed/33747742
http://dx.doi.org/10.1002/advs.202003611

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