Cargando…

Hairpin‐Spacer crRNA‐Enhanced CRISPR/Cas13a System Promotes the Specificity of Single Nucleotide Polymorphism (SNP) Identification

The Cas13a system has great potential in RNA interference and molecular diagnostic fields. However, lacking guidelines for crRNA design hinders practical applications of the Cas13a system in RNA editing and single nucleotide polymorphism identification. This study posits that crRNAs with hairpin spa...

Descripción completa

Detalles Bibliográficos
Autores principales:	Ke, Yuqing, Huang, Shiyi, Ghalandari, Behafarid, Li, Sijie, Warden, Antony R., Dang, Jingqi, Kang, Lin, Zhang, Yu, Wang, Yunqing, Sun, Yiqing, Wang, Jinglin, Cui, Daxiang, Zhi, Xiao, Ding, Xianting
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2021
Materias:	Full Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967054/ https://www.ncbi.nlm.nih.gov/pubmed/33747742 http://dx.doi.org/10.1002/advs.202003611

Ejemplares similares

2′-O-Methyl modified guide RNA promotes the single nucleotide polymorphism (SNP) discrimination ability of CRISPR–Cas12a systems
por: Ke, Yuqing, et al.
Publicado: (2022)

CRISPRTarget: Bioinformatic prediction and analysis of crRNA targets
por: Biswas, Ambarish, et al.
Publicado: (2013)

Improvement of the CRISPR-Cpf1 system with ribozyme-processed crRNA
por: Gao, Zongliang, et al.
Publicado: (2018)

Poly (N-vinylpyrrolidone) modification mitigates plasma protein corona formation on phosphomolybdate-based nanoparticles
por: Yu, Youyi, et al.
Publicado: (2021)

Extension of the crRNA enhances Cpf1 gene editing in vitro and in vivo
por: Park, Hyo Min, et al.
Publicado: (2018)

Programmable sequential mutagenesis by inducible Cpf1 crRNA array inversion
por: Chow, Ryan D., et al.
Publicado: (2018)

Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence
por: Ishibashi, Riki, et al.
Publicado: (2020)

CRISPRstrand: predicting repeat orientations to determine the crRNA-encoding strand at CRISPR loci
por: Alkhnbashi, Omer S., et al.
Publicado: (2014)

FnCas12a/crRNA-Mediated Genome Editing in Eimeria tenella
por: Cheng, Peipei, et al.
Publicado: (2021)

Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics
por: Hu, Menglu, et al.
Publicado: (2022)

Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a
por: Sinan, Selma, et al.
Publicado: (2023)

Genetic determinants of PAM-dependent DNA targeting and pre-crRNA processing in Sulfolobus islandicus
por: Peng, Wenfang, et al.
Publicado: (2013)

crRNA and tracrRNA guide Cas9-mediated DNA interference in Streptococcus thermophilus
por: Karvelis, Tautvydas, et al.
Publicado: (2013)

Features of CRISPR-Cas Regulation Key to Highly Efficient and Temporally-Specific crRNA Production
por: Rodic, Andjela, et al.
Publicado: (2017)

Improvement of the LbCas12a-crRNA System for Efficient Gene Targeting in Tomato
por: Vu, Tien Van, et al.
Publicado: (2021)

Asymmetric CRISPR enabling cascade signal amplification for nucleic acid detection by competitive crRNA
por: Moon, Jeong, et al.
Publicado: (2023)

Enhanced mammalian genome editing by new Cas12a orthologs with optimized crRNA scaffolds
por: Teng, Fei, et al.
Publicado: (2019)

Conformational Dynamics and Cleavage Sites of Cas12a Are Modulated by Complementarity between crRNA and DNA
por: Zhang, Lujia, et al.
Publicado: (2019)

Photoactivatable nanoCRISPR/Cas9 System Based on crRNA Reversibly Immobilized on Carbon Nanoparticles
por: Semikolenova, Olga, et al.
Publicado: (2021)

Multiplex gene editing by CRISPR-Cpf1 through autonomous processing of a single crRNA array
por: Zetsche, Bernd, et al.
Publicado: (2016)

New PAM Improves the Single-Base Specificity of crRNA-Guided LbCas12a Nuclease
por: Misiurina, Mariia A., et al.
Publicado: (2022)

A Facile Method to Synthesize 3D Pomegranate-like Polydopamine Microspheres
por: Ghorbani, Farnaz, et al.
Publicado: (2021)

Efficient CRISPR-Mediated Post-Transcriptional Gene Silencing in a Hyperthermophilic Archaeon Using Multiplexed crRNA Expression
por: Zebec, Ziga, et al.
Publicado: (2016)

Genetic editing and interrogation with Cpf1 and caged truncated pre-tRNA-like crRNA in mammalian cells
por: Zhang, Xuhua, et al.
Publicado: (2018)

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis
por: Liao, Chunyu, et al.
Publicado: (2019)

Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
por: Nguyen, Long T., et al.
Publicado: (2020)

Enhanced FnCas12a-Mediated Targeted Mutagenesis Using crRNA With Altered Target Length in Rice
por: Negishi, Katsuya, et al.
Publicado: (2020)

High-throughput screen reveals sRNAs regulating crRNA biogenesis by targeting CRISPR leader to repress Rho termination
por: Lin, Ping, et al.
Publicado: (2019)

Extinction of all infectious HIV in cell culture by the CRISPR-Cas12a system with only a single crRNA
por: Gao, Zongliang, et al.
Publicado: (2020)

The effect of crRNA–target mismatches on cOA-mediated interference by a type III-A CRISPR-Cas system
por: Nasef, Mohamed, et al.
Publicado: (2022)

Generation of the transgene-free canker-resistant Citrus sinensis using Cas12a/crRNA ribonucleoprotein in the T0 generation
por: Su, Hang, et al.
Publicado: (2023)

Author Correction: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
por: Nguyen, Long T., et al.
Publicado: (2020)

A rapid and efficient platform for antiviral crRNA screening using CRISPR-Cas13a-based nucleic acid detection
por: Yang, Lan, et al.
Publicado: (2023)

Cmr1 enables efficient RNA and DNA interference of a III-B CRISPR–Cas system by binding to target RNA and crRNA
por: Li, Yingjun, et al.
Publicado: (2017)

Structures of apo Cas12a and its complex with crRNA and DNA reveal the dynamics of ternary complex formation and target DNA cleavage
por: Jianwei, Li, et al.
Publicado: (2023)

In Vitro CRISPR-Cas12a-Based Detection of Cancer-Associated TP53 Hotspot Mutations Beyond the crRNA Seed Region
por: Kohabir, Kavish A.V., et al.
Publicado: (2023)

The in Silico Insight into Carbon Nanotube and Nucleic Acid Bases Interaction
por: Karimi, Ali Asghar, et al.
Publicado: (2016)

Injectable light-assisted thermo-responsive methylcellulose-sodium humate hydrogel proposed for photothermal ablation and localized delivery of cisplatin
por: Ghorbani, Farnaz, et al.
Publicado: (2022)

Editorial: Polydopamine-based structures innovation for surface engineering and Musculoskeletal Tissue Regeneration
por: Ghorbani, Farnaz, et al.
Publicado: (2023)

Stabilization of RNA hairpins using non-nucleotide linkers and circularization
por: Kiliszek, Agnieszka, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_jb1jcmc20erfvcqajolscvdpgn