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COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests

Healthcare workers are at the forefront against COVID-19, worldwide. Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative...

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Autores principales: Vetrugno, Giuseppe, La Milia, Daniele Ignazio, D’Ambrosio, Floriana, Di Pumpo, Marcello, Pastorino, Roberta, Boccia, Stefania, Ricci, Rosalba, De-Giorgio, Fabio, Cicconi, Michela, Foti, Federica, Pascucci, Domenico, Castrini, Francesco, Carini, Elettra, Cambieri, Andrea, D’Alfonso, Maria Elena, Capalbo, Gennaro, Fantoni, Massimo, Moscato, Umberto, Staiti, Domenico, De Simone, Francesco Maria, Berloco, Filippo, Damiani, Gianfranco, Zega, Maurizio, Cattani, Paola, Posteraro, Brunella, Sanguinetti, Maurizio, Laurenti, Patrizia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967358/
https://www.ncbi.nlm.nih.gov/pubmed/33800721
http://dx.doi.org/10.3390/ijerph18052650
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author Vetrugno, Giuseppe
La Milia, Daniele Ignazio
D’Ambrosio, Floriana
Di Pumpo, Marcello
Pastorino, Roberta
Boccia, Stefania
Ricci, Rosalba
De-Giorgio, Fabio
Cicconi, Michela
Foti, Federica
Pascucci, Domenico
Castrini, Francesco
Carini, Elettra
Cambieri, Andrea
D’Alfonso, Maria Elena
Capalbo, Gennaro
Fantoni, Massimo
Moscato, Umberto
Staiti, Domenico
De Simone, Francesco Maria
Berloco, Filippo
Damiani, Gianfranco
Zega, Maurizio
Cattani, Paola
Posteraro, Brunella
Sanguinetti, Maurizio
Laurenti, Patrizia
author_facet Vetrugno, Giuseppe
La Milia, Daniele Ignazio
D’Ambrosio, Floriana
Di Pumpo, Marcello
Pastorino, Roberta
Boccia, Stefania
Ricci, Rosalba
De-Giorgio, Fabio
Cicconi, Michela
Foti, Federica
Pascucci, Domenico
Castrini, Francesco
Carini, Elettra
Cambieri, Andrea
D’Alfonso, Maria Elena
Capalbo, Gennaro
Fantoni, Massimo
Moscato, Umberto
Staiti, Domenico
De Simone, Francesco Maria
Berloco, Filippo
Damiani, Gianfranco
Zega, Maurizio
Cattani, Paola
Posteraro, Brunella
Sanguinetti, Maurizio
Laurenti, Patrizia
author_sort Vetrugno, Giuseppe
collection PubMed
description Healthcare workers are at the forefront against COVID-19, worldwide. Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative staff were designated to work from home. Between 4 June and 3 July 2020, an investigation was conducted to evaluate the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin (IgG) antibodies among the employees of the FPG using point-of-care (POC) and venous blood tests. Sensitivity, specificity, and predictive values were determined with reverse-transcription polymerase chain reaction on nasal/oropharyngeal swabs as the diagnostic gold standard. The participants enrolled amounted to 4777. Seroprevalence was 3.66% using the POC test and 1.19% using the venous blood test, with a significant difference (p < 0.05). The POC test sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among the low-risk populations, the POC test’s predictive values were 58.33% (positive) and 98.23% (negative), whereas those of the venous blood test were 92.86% (positive) and 98.53% (negative). According to our study, these serological tests cannot be a valid alternative to diagnose COVID-19 infection in progress.
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spelling pubmed-79673582021-03-18 COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests Vetrugno, Giuseppe La Milia, Daniele Ignazio D’Ambrosio, Floriana Di Pumpo, Marcello Pastorino, Roberta Boccia, Stefania Ricci, Rosalba De-Giorgio, Fabio Cicconi, Michela Foti, Federica Pascucci, Domenico Castrini, Francesco Carini, Elettra Cambieri, Andrea D’Alfonso, Maria Elena Capalbo, Gennaro Fantoni, Massimo Moscato, Umberto Staiti, Domenico De Simone, Francesco Maria Berloco, Filippo Damiani, Gianfranco Zega, Maurizio Cattani, Paola Posteraro, Brunella Sanguinetti, Maurizio Laurenti, Patrizia Int J Environ Res Public Health Article Healthcare workers are at the forefront against COVID-19, worldwide. Since Fondazione Policlinico Universitario A. Gemelli (FPG) IRCCS was enlisted as a COVID-19 hospital, the healthcare workers deployed to COVID-19 wards were separated from those with limited/no exposure, whereas the administrative staff were designated to work from home. Between 4 June and 3 July 2020, an investigation was conducted to evaluate the seroprevalence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) immunoglobulin (IgG) antibodies among the employees of the FPG using point-of-care (POC) and venous blood tests. Sensitivity, specificity, and predictive values were determined with reverse-transcription polymerase chain reaction on nasal/oropharyngeal swabs as the diagnostic gold standard. The participants enrolled amounted to 4777. Seroprevalence was 3.66% using the POC test and 1.19% using the venous blood test, with a significant difference (p < 0.05). The POC test sensitivity and specificity were, respectively, 63.64% (95% confidence interval (CI): 62.20% to 65.04%) and 96.64% (95% CI: 96.05% to 97.13%), while those of the venous blood test were, respectively, 78.79% (95% CI: 77.58% to 79.94%) and 99.36% (95% CI: 99.07% to 99.55%). Among the low-risk populations, the POC test’s predictive values were 58.33% (positive) and 98.23% (negative), whereas those of the venous blood test were 92.86% (positive) and 98.53% (negative). According to our study, these serological tests cannot be a valid alternative to diagnose COVID-19 infection in progress. MDPI 2021-03-06 /pmc/articles/PMC7967358/ /pubmed/33800721 http://dx.doi.org/10.3390/ijerph18052650 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vetrugno, Giuseppe
La Milia, Daniele Ignazio
D’Ambrosio, Floriana
Di Pumpo, Marcello
Pastorino, Roberta
Boccia, Stefania
Ricci, Rosalba
De-Giorgio, Fabio
Cicconi, Michela
Foti, Federica
Pascucci, Domenico
Castrini, Francesco
Carini, Elettra
Cambieri, Andrea
D’Alfonso, Maria Elena
Capalbo, Gennaro
Fantoni, Massimo
Moscato, Umberto
Staiti, Domenico
De Simone, Francesco Maria
Berloco, Filippo
Damiani, Gianfranco
Zega, Maurizio
Cattani, Paola
Posteraro, Brunella
Sanguinetti, Maurizio
Laurenti, Patrizia
COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
title COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
title_full COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
title_fullStr COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
title_full_unstemmed COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
title_short COVID-19 Seroprevalence among Healthcare Workers of a Large COVID-19 Hospital in Rome Reveals Strengths and Limits of Two Different Serological Tests
title_sort covid-19 seroprevalence among healthcare workers of a large covid-19 hospital in rome reveals strengths and limits of two different serological tests
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967358/
https://www.ncbi.nlm.nih.gov/pubmed/33800721
http://dx.doi.org/10.3390/ijerph18052650
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