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A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture

Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination...

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Autores principales: Sivanesan, Iyyakkannu, Muthu, Manikandan, Gopal, Judy, Tasneem, Shadma, Kim, Doo-Hwan, Oh, Jae-Wook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967719/
https://www.ncbi.nlm.nih.gov/pubmed/33668989
http://dx.doi.org/10.3390/ijerph18052282
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author Sivanesan, Iyyakkannu
Muthu, Manikandan
Gopal, Judy
Tasneem, Shadma
Kim, Doo-Hwan
Oh, Jae-Wook
author_facet Sivanesan, Iyyakkannu
Muthu, Manikandan
Gopal, Judy
Tasneem, Shadma
Kim, Doo-Hwan
Oh, Jae-Wook
author_sort Sivanesan, Iyyakkannu
collection PubMed
description Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel. The successful initiation of in vitro culture mostly depends on surface sterilization of explants because this is the primary source. Usually, surface sterilization is done using chemicals, or toxic nanomaterials, this is the first time such an approach has been demonstrated. Numerous surface microflora attached to plant surfaces grow faster than the cultured explants and release phytotoxic substances into the culture media, hindering positive outcomes. In the current work, for the first time, the applicability of turmeric and benzoin resin-based fumigation of explants is demonstrated. The results showed that fumigation methods for surface sterilization were promising and could lead to fifty and even 100% contamination-free plant tissue culture. Nanoparticulate carbon was identified in the turmeric and benzoin smoke and coined the key player in the surface sterilization effect. These studies open a whole new avenue for the use of fumigation-based methods for riddance of microbial contamination.
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spelling pubmed-79677192021-03-18 A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture Sivanesan, Iyyakkannu Muthu, Manikandan Gopal, Judy Tasneem, Shadma Kim, Doo-Hwan Oh, Jae-Wook Int J Environ Res Public Health Article Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel. The successful initiation of in vitro culture mostly depends on surface sterilization of explants because this is the primary source. Usually, surface sterilization is done using chemicals, or toxic nanomaterials, this is the first time such an approach has been demonstrated. Numerous surface microflora attached to plant surfaces grow faster than the cultured explants and release phytotoxic substances into the culture media, hindering positive outcomes. In the current work, for the first time, the applicability of turmeric and benzoin resin-based fumigation of explants is demonstrated. The results showed that fumigation methods for surface sterilization were promising and could lead to fifty and even 100% contamination-free plant tissue culture. Nanoparticulate carbon was identified in the turmeric and benzoin smoke and coined the key player in the surface sterilization effect. These studies open a whole new avenue for the use of fumigation-based methods for riddance of microbial contamination. MDPI 2021-02-25 /pmc/articles/PMC7967719/ /pubmed/33668989 http://dx.doi.org/10.3390/ijerph18052282 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sivanesan, Iyyakkannu
Muthu, Manikandan
Gopal, Judy
Tasneem, Shadma
Kim, Doo-Hwan
Oh, Jae-Wook
A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
title A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
title_full A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
title_fullStr A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
title_full_unstemmed A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
title_short A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
title_sort fumigation-based surface sterilization approach for plant tissue culture
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967719/
https://www.ncbi.nlm.nih.gov/pubmed/33668989
http://dx.doi.org/10.3390/ijerph18052282
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