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A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture
Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967719/ https://www.ncbi.nlm.nih.gov/pubmed/33668989 http://dx.doi.org/10.3390/ijerph18052282 |
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author | Sivanesan, Iyyakkannu Muthu, Manikandan Gopal, Judy Tasneem, Shadma Kim, Doo-Hwan Oh, Jae-Wook |
author_facet | Sivanesan, Iyyakkannu Muthu, Manikandan Gopal, Judy Tasneem, Shadma Kim, Doo-Hwan Oh, Jae-Wook |
author_sort | Sivanesan, Iyyakkannu |
collection | PubMed |
description | Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel. The successful initiation of in vitro culture mostly depends on surface sterilization of explants because this is the primary source. Usually, surface sterilization is done using chemicals, or toxic nanomaterials, this is the first time such an approach has been demonstrated. Numerous surface microflora attached to plant surfaces grow faster than the cultured explants and release phytotoxic substances into the culture media, hindering positive outcomes. In the current work, for the first time, the applicability of turmeric and benzoin resin-based fumigation of explants is demonstrated. The results showed that fumigation methods for surface sterilization were promising and could lead to fifty and even 100% contamination-free plant tissue culture. Nanoparticulate carbon was identified in the turmeric and benzoin smoke and coined the key player in the surface sterilization effect. These studies open a whole new avenue for the use of fumigation-based methods for riddance of microbial contamination. |
format | Online Article Text |
id | pubmed-7967719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-79677192021-03-18 A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture Sivanesan, Iyyakkannu Muthu, Manikandan Gopal, Judy Tasneem, Shadma Kim, Doo-Hwan Oh, Jae-Wook Int J Environ Res Public Health Article Plant tissue culture has led to breakthroughs in understanding and applying the fundamental knowledge towards harnessing more from plants. Microbial contamination is one of the serious problems limiting the successful extrapolation of plant tissue culture practices. Sources of in vitro contamination include culture containers, media, explants, equipment, the environment of the culture room and transfer area, and operating personnel. The successful initiation of in vitro culture mostly depends on surface sterilization of explants because this is the primary source. Usually, surface sterilization is done using chemicals, or toxic nanomaterials, this is the first time such an approach has been demonstrated. Numerous surface microflora attached to plant surfaces grow faster than the cultured explants and release phytotoxic substances into the culture media, hindering positive outcomes. In the current work, for the first time, the applicability of turmeric and benzoin resin-based fumigation of explants is demonstrated. The results showed that fumigation methods for surface sterilization were promising and could lead to fifty and even 100% contamination-free plant tissue culture. Nanoparticulate carbon was identified in the turmeric and benzoin smoke and coined the key player in the surface sterilization effect. These studies open a whole new avenue for the use of fumigation-based methods for riddance of microbial contamination. MDPI 2021-02-25 /pmc/articles/PMC7967719/ /pubmed/33668989 http://dx.doi.org/10.3390/ijerph18052282 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sivanesan, Iyyakkannu Muthu, Manikandan Gopal, Judy Tasneem, Shadma Kim, Doo-Hwan Oh, Jae-Wook A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture |
title | A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture |
title_full | A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture |
title_fullStr | A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture |
title_full_unstemmed | A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture |
title_short | A Fumigation-Based Surface Sterilization Approach for Plant Tissue Culture |
title_sort | fumigation-based surface sterilization approach for plant tissue culture |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7967719/ https://www.ncbi.nlm.nih.gov/pubmed/33668989 http://dx.doi.org/10.3390/ijerph18052282 |
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