Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network

AIM: This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. MATERIAL AND METHODS: A total of 52 centers participated, 43 hospital-based laboratories...

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Detalles Bibliográficos
Autores principales: Garza-González, Elvira, Bocanegra-Ibarias, Paola, Bobadilla-del-Valle, Miriam, Ponce-de-León-Garduño, Luis Alfredo, Esteban-Kenel, Verónica, Silva-Sánchez, Jesus, Garza-Ramos, Ulises, Barrios-Camacho, Humberto, López-Jácome, Luis Esaú, Colin-Castro, Claudia A., Franco-Cendejas, Rafael, Flores-Treviño, Samantha, Morfín-Otero, Rayo, Rojas-Larios, Fabian, Mena-Ramírez, Juan Pablo, Fong-Camargo, María Guadalupe, Morales-De-la-Peña, Cecilia Teresita, García-Mendoza, Lourdes, Choy-Chang, Elena Victoria, Aviles-Benitez, Laura Karina, Feliciano-Guzmán, José Manuel, López-Gutiérrez, Eduardo, Gil-Veloz, Mariana, Barajas-Magallón, Juan Manuel, Aguirre-Burciaga, Efren, López-Moreno, Laura Isabel, Martínez-Villarreal, Rebeca Thelma, Canizales-Oviedo, Jorge Luis, Cetina-Umaña, Carlos Miguel, Romero-Romero, Daniel, Bello-Pazos, Fidencio David, Barlandas-Rendón, Nicolás Rogelio Eric, Maldonado-Anicacio, Joyarib Yanelli, Bolado-Martínez, Enrique, Galindo-Méndez, Mario, Perez-Vicelis, Talia, Alavez-Ramírez, Norma, Méndez-Sotelo, Braulio J., Cabriales-Zavala, Juan Francisco, Nava-Pacheco, Yirla Citlali, Moreno-Méndez, Martha Irene, García-Romo, Ricardo, Silva-Gamiño, Aldo Rafael, Avalos-Aguilera, Ana María, Santiago-Calderón, María Asunción, López-García, Maribel, Velázquez-Acosta, María del Consuelo, Cobos-Canul, Dulce Isabel, Vázquez-Larios, María del Rosario, Ortiz-Porcayo, Ana Elizabeth, Guerrero-Núñez, Arely Elizabeth, Valero-Guzmán, Jazmín, Rosales-García, Alina Aracely, Ostos-Cantú, Heidy Leticia, Camacho-Ortiz, Adrián
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968647/
https://www.ncbi.nlm.nih.gov/pubmed/33730101
http://dx.doi.org/10.1371/journal.pone.0248614
Descripción
Sumario:AIM: This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum β-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. MATERIAL AND METHODS: A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae, ESBL or carbapenem-resistant E. coli, and K. pneumoniae, carbapenem-resistant A. baumannii complex, and P. aeruginosa. Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes. RESULTS: Among blood isolates, A. baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E. cloacae complex showed higher resistance to carbapenems. A. baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K. pneumoniae isolates, bla(TEM), bla(SHV), and bla(CTX) were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E. coli isolates, bla(TEM), bla(SHV), and bla(CTX) were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was bla(CTX-M-15). Among Enterobacteriaceae, the most frequently detected carbapenemase-encoding gene was bla(NDM-1) (81.5%), followed by bla(OXA-232) (14.8%) and bla(oxa-181)(7.4%), in A. baumannii was bla(OXA-24) (76%) and in P. aeruginosa, was bla(IMP) (25.3%), followed by bla(GES) and bla(VIM) (13.1% each). CONCLUSION: Our study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL bla(CTX-M-15) exists in both E. coli and K. pneumoniae.

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