Cargando…

Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission

Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like viruses, with features similar to those of the Totiviridae family. Most reported members of this family...

Descripción completa

Detalles Bibliográficos
Autores principales: Shao, Qianqian, Jia, Xudong, Gao, Yuanzhu, Liu, Zhe, Zhang, Huan, Tan, Qiqi, Zhang, Xin, Zhou, Huiqiong, Li, Yinyin, Wu, De, Zhang, Qinfen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968656/
https://www.ncbi.nlm.nih.gov/pubmed/33730056
http://dx.doi.org/10.1371/journal.ppat.1009396
_version_ 1783666107233599488
author Shao, Qianqian
Jia, Xudong
Gao, Yuanzhu
Liu, Zhe
Zhang, Huan
Tan, Qiqi
Zhang, Xin
Zhou, Huiqiong
Li, Yinyin
Wu, De
Zhang, Qinfen
author_facet Shao, Qianqian
Jia, Xudong
Gao, Yuanzhu
Liu, Zhe
Zhang, Huan
Tan, Qiqi
Zhang, Xin
Zhou, Huiqiong
Li, Yinyin
Wu, De
Zhang, Qinfen
author_sort Shao, Qianqian
collection PubMed
description Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like viruses, with features similar to those of the Totiviridae family. Most reported members of this family infect fungi or protozoans and lack an extracellular life cycle stage. Here, we identified a new strain of OmRV and determined high-resolution structures for this virus using single-particle cryo-electron microscopy. The structures feature an unexpected protrusion at the five-fold vertex of the capsid. Disassociation of the protrusion could result in several conformational changes in the major capsid. All these structures, together with some biological results, suggest the protrusions’ associations with the extracellular transmission of OmRV.
format Online
Article
Text
id pubmed-7968656
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-79686562021-03-31 Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission Shao, Qianqian Jia, Xudong Gao, Yuanzhu Liu, Zhe Zhang, Huan Tan, Qiqi Zhang, Xin Zhou, Huiqiong Li, Yinyin Wu, De Zhang, Qinfen PLoS Pathog Research Article Mosquito viruses cause unpredictable outbreaks of disease. Recently, several unassigned viruses isolated from mosquitoes, including the Omono River virus (OmRV), were identified as totivirus-like viruses, with features similar to those of the Totiviridae family. Most reported members of this family infect fungi or protozoans and lack an extracellular life cycle stage. Here, we identified a new strain of OmRV and determined high-resolution structures for this virus using single-particle cryo-electron microscopy. The structures feature an unexpected protrusion at the five-fold vertex of the capsid. Disassociation of the protrusion could result in several conformational changes in the major capsid. All these structures, together with some biological results, suggest the protrusions’ associations with the extracellular transmission of OmRV. Public Library of Science 2021-03-17 /pmc/articles/PMC7968656/ /pubmed/33730056 http://dx.doi.org/10.1371/journal.ppat.1009396 Text en © 2021 Shao et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shao, Qianqian
Jia, Xudong
Gao, Yuanzhu
Liu, Zhe
Zhang, Huan
Tan, Qiqi
Zhang, Xin
Zhou, Huiqiong
Li, Yinyin
Wu, De
Zhang, Qinfen
Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission
title Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission
title_full Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission
title_fullStr Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission
title_full_unstemmed Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission
title_short Cryo-EM reveals a previously unrecognized structural protein of a dsRNA virus implicated in its extracellular transmission
title_sort cryo-em reveals a previously unrecognized structural protein of a dsrna virus implicated in its extracellular transmission
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968656/
https://www.ncbi.nlm.nih.gov/pubmed/33730056
http://dx.doi.org/10.1371/journal.ppat.1009396
work_keys_str_mv AT shaoqianqian cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT jiaxudong cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT gaoyuanzhu cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT liuzhe cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT zhanghuan cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT tanqiqi cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT zhangxin cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT zhouhuiqiong cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT liyinyin cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT wude cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission
AT zhangqinfen cryoemrevealsapreviouslyunrecognizedstructuralproteinofadsrnavirusimplicatedinitsextracellulartransmission