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Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine

The occurrence of nontuberculous mycobacteria in different hosts and their implication as obligate or opportunistic pathogens remain mainly unclear. Mycobacteriosis in pigs is usually associated with members of the Mycobacterium avium complex and, in particular, with ‘ Mycobacterium avium subsp. hom...

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Autores principales: Ghielmetti, Giovanni, Rosato, Giuliana, Trovato, Alberto, Friedel, Ute, Kirchgaessner, Constanze, Perroulaz, Carmen, Pendl, Wolfgang, Schulthess, Bettina, Bloemberg, Guido V., Keller, Peter M., Stephan, Roger, Tortoli, Enrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Microbiology Society 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968739/
https://www.ncbi.nlm.nih.gov/pubmed/33355527
http://dx.doi.org/10.1099/ijsem.0.004615
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author Ghielmetti, Giovanni
Rosato, Giuliana
Trovato, Alberto
Friedel, Ute
Kirchgaessner, Constanze
Perroulaz, Carmen
Pendl, Wolfgang
Schulthess, Bettina
Bloemberg, Guido V.
Keller, Peter M.
Stephan, Roger
Tortoli, Enrico
author_facet Ghielmetti, Giovanni
Rosato, Giuliana
Trovato, Alberto
Friedel, Ute
Kirchgaessner, Constanze
Perroulaz, Carmen
Pendl, Wolfgang
Schulthess, Bettina
Bloemberg, Guido V.
Keller, Peter M.
Stephan, Roger
Tortoli, Enrico
author_sort Ghielmetti, Giovanni
collection PubMed
description The occurrence of nontuberculous mycobacteria in different hosts and their implication as obligate or opportunistic pathogens remain mainly unclear. Mycobacteriosis in pigs is usually associated with members of the Mycobacterium avium complex and, in particular, with ‘ Mycobacterium avium subsp. hominissuis ’. Here we describe a novel slow-growing mycobacterial species isolated from lymph nodes obtained from two sows housed in different Swiss farms. The animals presented chronic inappetence and mild diarrhoea. Gross pathology revealed focal caseous lymphadenopathy of the mesenteric lymph nodes. Complete genome sequencing of the two isolates from the two sows was performed. The genomes comprised 5.76 Mb and an average nucleotide identity score of 99.97 %. Whole genome sequence, mycolic acid and matrix-assisted laser desorption ionization-time of flight mass spectrometry analyses revealed that the two isolates were not related to any previously described Mycobacterium species. The closest related species was Mycobacterium parmense , a slow-growing scotochromogenic mycobacterium first isolated from a cervical lymph node of a 3-year-old child. The name proposed for the new species is Mycobacterium helveticum sp. nov. and 16-83(T) (=DSM 109965(T)= LMG 2019-02457(T)) is the type strain.
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spelling pubmed-79687392021-03-19 Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine Ghielmetti, Giovanni Rosato, Giuliana Trovato, Alberto Friedel, Ute Kirchgaessner, Constanze Perroulaz, Carmen Pendl, Wolfgang Schulthess, Bettina Bloemberg, Guido V. Keller, Peter M. Stephan, Roger Tortoli, Enrico Int J Syst Evol Microbiol Taxonomic Description The occurrence of nontuberculous mycobacteria in different hosts and their implication as obligate or opportunistic pathogens remain mainly unclear. Mycobacteriosis in pigs is usually associated with members of the Mycobacterium avium complex and, in particular, with ‘ Mycobacterium avium subsp. hominissuis ’. Here we describe a novel slow-growing mycobacterial species isolated from lymph nodes obtained from two sows housed in different Swiss farms. The animals presented chronic inappetence and mild diarrhoea. Gross pathology revealed focal caseous lymphadenopathy of the mesenteric lymph nodes. Complete genome sequencing of the two isolates from the two sows was performed. The genomes comprised 5.76 Mb and an average nucleotide identity score of 99.97 %. Whole genome sequence, mycolic acid and matrix-assisted laser desorption ionization-time of flight mass spectrometry analyses revealed that the two isolates were not related to any previously described Mycobacterium species. The closest related species was Mycobacterium parmense , a slow-growing scotochromogenic mycobacterium first isolated from a cervical lymph node of a 3-year-old child. The name proposed for the new species is Mycobacterium helveticum sp. nov. and 16-83(T) (=DSM 109965(T)= LMG 2019-02457(T)) is the type strain. Microbiology Society 2021-01 2020-12-23 /pmc/articles/PMC7968739/ /pubmed/33355527 http://dx.doi.org/10.1099/ijsem.0.004615 Text en © 2021 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License.
spellingShingle Taxonomic Description
Ghielmetti, Giovanni
Rosato, Giuliana
Trovato, Alberto
Friedel, Ute
Kirchgaessner, Constanze
Perroulaz, Carmen
Pendl, Wolfgang
Schulthess, Bettina
Bloemberg, Guido V.
Keller, Peter M.
Stephan, Roger
Tortoli, Enrico
Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
title Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
title_full Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
title_fullStr Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
title_full_unstemmed Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
title_short Mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
title_sort mycobacterium helveticum sp. nov., a novel slowly growing mycobacterial species associated with granulomatous lesions in adult swine
topic Taxonomic Description
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968739/
https://www.ncbi.nlm.nih.gov/pubmed/33355527
http://dx.doi.org/10.1099/ijsem.0.004615
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