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A high-throughput screening method for evolving a demethylase enzyme with improved and new functionalities

AlkB is a DNA/RNA repair enzyme that removes base alkylations such as N(1)-methyladenosine (m(1)A) or N(3)-methylcytosine (m(3)C) from DNA and RNA. The AlkB enzyme has been used as a critical tool to facilitate tRNA sequencing and identification of mRNA modifications. As a tool, AlkB mutants with be...

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Detalles Bibliográficos
Autores principales:	Wang, Yuru, Katanski, Christopher D, Watkins, Christopher, Pan, Jessica N, Dai, Qing, Jiang, Zhuoxun, Pan, Tao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2020
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968990/ https://www.ncbi.nlm.nih.gov/pubmed/33337498 http://dx.doi.org/10.1093/nar/gkaa1213

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