HuB and HuD repress telomerase activity by dissociating HuR from TERC

The ubiquitous RNA-binding protein HuR (ELAVL1) promotes telomerase activity by associating with the telomerase noncoding RNA TERC. However, the role of the neural-specific members HuB, HuC, and HuD (ELAVL2–4) in telomerase activity is unknown. Here, we report that HuB and HuD, but not HuC, repress...

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Autores principales: Cheng, Xiaolei, Gu, Xiaoping, Xia, Tianjiao, Ma, Zhengliang, Yang, Zhongzhou, Feng, Helen Lechen, Zhao, Yong, Ma, Wenbin, Ju, Zhenyu, Gorospe, Myriam, Yi, Xia, Tang, Hao, Wang, Wengong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
RNA and RNA-protein complexes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969021/
https://www.ncbi.nlm.nih.gov/pubmed/33589924
http://dx.doi.org/10.1093/nar/gkab062
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author Cheng, Xiaolei
Gu, Xiaoping
Xia, Tianjiao
Ma, Zhengliang
Yang, Zhongzhou
Feng, Helen Lechen
Zhao, Yong
Ma, Wenbin
Ju, Zhenyu
Gorospe, Myriam
Yi, Xia
Tang, Hao
Wang, Wengong
author_facet Cheng, Xiaolei
Gu, Xiaoping
Xia, Tianjiao
Ma, Zhengliang
Yang, Zhongzhou
Feng, Helen Lechen
Zhao, Yong
Ma, Wenbin
Ju, Zhenyu
Gorospe, Myriam
Yi, Xia
Tang, Hao
Wang, Wengong
author_sort Cheng, Xiaolei
collection PubMed
description The ubiquitous RNA-binding protein HuR (ELAVL1) promotes telomerase activity by associating with the telomerase noncoding RNA TERC. However, the role of the neural-specific members HuB, HuC, and HuD (ELAVL2–4) in telomerase activity is unknown. Here, we report that HuB and HuD, but not HuC, repress telomerase activity in human neuroblastoma cells. By associating with AU-rich sequences in TERC, HuB and HuD repressed the assembly of the TERT–TERC core complex. Furthermore, HuB and HuD competed with HuR for binding to TERC and antagonized the function of HuR that was previously shown to enhance telomerase activity to promote cell growth. Our findings reveal a novel mechanism controlling telomerase activity in human neuroblastoma cells that involves a competition between HuR and the related, neural-specific proteins HuB and HuD.
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spelling pubmed-79690212021-03-22 HuB and HuD repress telomerase activity by dissociating HuR from TERC Cheng, Xiaolei Gu, Xiaoping Xia, Tianjiao Ma, Zhengliang Yang, Zhongzhou Feng, Helen Lechen Zhao, Yong Ma, Wenbin Ju, Zhenyu Gorospe, Myriam Yi, Xia Tang, Hao Wang, Wengong Nucleic Acids Res RNA and RNA-protein complexes The ubiquitous RNA-binding protein HuR (ELAVL1) promotes telomerase activity by associating with the telomerase noncoding RNA TERC. However, the role of the neural-specific members HuB, HuC, and HuD (ELAVL2–4) in telomerase activity is unknown. Here, we report that HuB and HuD, but not HuC, repress telomerase activity in human neuroblastoma cells. By associating with AU-rich sequences in TERC, HuB and HuD repressed the assembly of the TERT–TERC core complex. Furthermore, HuB and HuD competed with HuR for binding to TERC and antagonized the function of HuR that was previously shown to enhance telomerase activity to promote cell growth. Our findings reveal a novel mechanism controlling telomerase activity in human neuroblastoma cells that involves a competition between HuR and the related, neural-specific proteins HuB and HuD. Oxford University Press 2021-02-15 /pmc/articles/PMC7969021/ /pubmed/33589924 http://dx.doi.org/10.1093/nar/gkab062 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle RNA and RNA-protein complexes
Cheng, Xiaolei
Gu, Xiaoping
Xia, Tianjiao
Ma, Zhengliang
Yang, Zhongzhou
Feng, Helen Lechen
Zhao, Yong
Ma, Wenbin
Ju, Zhenyu
Gorospe, Myriam
Yi, Xia
Tang, Hao
Wang, Wengong
HuB and HuD repress telomerase activity by dissociating HuR from TERC
title HuB and HuD repress telomerase activity by dissociating HuR from TERC
title_full HuB and HuD repress telomerase activity by dissociating HuR from TERC
title_fullStr HuB and HuD repress telomerase activity by dissociating HuR from TERC
title_full_unstemmed HuB and HuD repress telomerase activity by dissociating HuR from TERC
title_short HuB and HuD repress telomerase activity by dissociating HuR from TERC
title_sort hub and hud repress telomerase activity by dissociating hur from terc
topic RNA and RNA-protein complexes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969021/
https://www.ncbi.nlm.nih.gov/pubmed/33589924
http://dx.doi.org/10.1093/nar/gkab062
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