Nile Red Incubation Time Before Reading Fluorescence Greatly Influences the Yeast Neutral Lipids Quantification

High-throughput screening methodologies to estimate lipid content in oleaginous yeasts use Nile red fluorescence in a given solvent and optimized excitation/emission wavelengths. However, Nile red fluorescence stabilization has been poorly analyzed, and high variability occurs when relative fluoresc...

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Detalles Bibliográficos
Autores principales: Ramírez-Castrillón, Mauricio, Jaramillo-Garcia, Victoria P., Lopes Barros, Helio, Pegas Henriques, João A., Stefani, Valter, Valente, Patricia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Microbiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969498/
https://www.ncbi.nlm.nih.gov/pubmed/33746916
http://dx.doi.org/10.3389/fmicb.2021.619313

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969498/
https://www.ncbi.nlm.nih.gov/pubmed/33746916
http://dx.doi.org/10.3389/fmicb.2021.619313

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