Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src

Rab proteins play crucial roles in membrane trafficking. Some Rab proteins are implicated in cancer development through regulating protein sorting or degradation. In this study, we found that the expression of Rab26 is suppressed in the aggressive breast cancer cells as compared to the levels in non...

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Autores principales: Liu, Huiying, Zhou, Yuxia, Qiu, Hantian, Zhuang, Ruijuan, Han, Yang, Liu, Xiaoqing, Qiu, Xi, Wang, Ziyan, Xu, Liju, Tan, Ran, Hong, Wanjin, Wang, Tuanlao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969620/
https://www.ncbi.nlm.nih.gov/pubmed/33731709
http://dx.doi.org/10.1038/s41419-021-03561-7
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author Liu, Huiying
Zhou, Yuxia
Qiu, Hantian
Zhuang, Ruijuan
Han, Yang
Liu, Xiaoqing
Qiu, Xi
Wang, Ziyan
Xu, Liju
Tan, Ran
Hong, Wanjin
Wang, Tuanlao
author_facet Liu, Huiying
Zhou, Yuxia
Qiu, Hantian
Zhuang, Ruijuan
Han, Yang
Liu, Xiaoqing
Qiu, Xi
Wang, Ziyan
Xu, Liju
Tan, Ran
Hong, Wanjin
Wang, Tuanlao
author_sort Liu, Huiying
collection PubMed
description Rab proteins play crucial roles in membrane trafficking. Some Rab proteins are implicated in cancer development through regulating protein sorting or degradation. In this study, we found that the expression of Rab26 is suppressed in the aggressive breast cancer cells as compared to the levels in non-invasive breast cancer cells. Over-expression of Rab26 inhibits cell migration and invasion, while Rab26 knockdown significantly promotes the migration and invasion of breast cancer cells. Rab26 reduces focal adhesion association of Src kinase and induces endosomal translocation of Src. Further experiments revealed that Rab26 mediates the autophagic degradation of phosphorylated Src through interacting with ATG16L1, consequently, resulting in the suppression of the migration and invasion ability of breast cancer cells.
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spelling pubmed-79696202021-04-01 Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src Liu, Huiying Zhou, Yuxia Qiu, Hantian Zhuang, Ruijuan Han, Yang Liu, Xiaoqing Qiu, Xi Wang, Ziyan Xu, Liju Tan, Ran Hong, Wanjin Wang, Tuanlao Cell Death Dis Article Rab proteins play crucial roles in membrane trafficking. Some Rab proteins are implicated in cancer development through regulating protein sorting or degradation. In this study, we found that the expression of Rab26 is suppressed in the aggressive breast cancer cells as compared to the levels in non-invasive breast cancer cells. Over-expression of Rab26 inhibits cell migration and invasion, while Rab26 knockdown significantly promotes the migration and invasion of breast cancer cells. Rab26 reduces focal adhesion association of Src kinase and induces endosomal translocation of Src. Further experiments revealed that Rab26 mediates the autophagic degradation of phosphorylated Src through interacting with ATG16L1, consequently, resulting in the suppression of the migration and invasion ability of breast cancer cells. Nature Publishing Group UK 2021-03-17 /pmc/articles/PMC7969620/ /pubmed/33731709 http://dx.doi.org/10.1038/s41419-021-03561-7 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Liu, Huiying
Zhou, Yuxia
Qiu, Hantian
Zhuang, Ruijuan
Han, Yang
Liu, Xiaoqing
Qiu, Xi
Wang, Ziyan
Xu, Liju
Tan, Ran
Hong, Wanjin
Wang, Tuanlao
Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src
title Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src
title_full Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src
title_fullStr Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src
title_full_unstemmed Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src
title_short Rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated Src
title_sort rab26 suppresses migration and invasion of breast cancer cells through mediating autophagic degradation of phosphorylated src
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969620/
https://www.ncbi.nlm.nih.gov/pubmed/33731709
http://dx.doi.org/10.1038/s41419-021-03561-7
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