Cargando…

Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018

Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease and brings huge economic losses to commercial pork production worldwide. PRRSV causes severe reproductive failure in sows and respiratory distress in piglets. To trace the evolution of PRRSV in pigs with r...

Descripción completa

Detalles Bibliográficos
Autores principales: Yin, Baishuang, Qi, Shanshan, Sha, Wanli, Qin, Hongyu, Liu, Liming, Yun, Jinyan, Zhu, Jinhai, Li, Guojiang, Sun, Dongbo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969665/
https://www.ncbi.nlm.nih.gov/pubmed/33748205
http://dx.doi.org/10.3389/fvets.2021.605832
_version_ 1783666273096302592
author Yin, Baishuang
Qi, Shanshan
Sha, Wanli
Qin, Hongyu
Liu, Liming
Yun, Jinyan
Zhu, Jinhai
Li, Guojiang
Sun, Dongbo
author_facet Yin, Baishuang
Qi, Shanshan
Sha, Wanli
Qin, Hongyu
Liu, Liming
Yun, Jinyan
Zhu, Jinhai
Li, Guojiang
Sun, Dongbo
author_sort Yin, Baishuang
collection PubMed
description Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease and brings huge economic losses to commercial pork production worldwide. PRRSV causes severe reproductive failure in sows and respiratory distress in piglets. To trace the evolution of PRRSV in pigs with respiratory diseases in some regions of China, 112 samples were collected from nine provinces in China during 2016–2018. All samples were detected by RT-PCR and analyzed by the Nsp2/ORF5 (ORF5a)-genes-phylogeny. Sequence analysis and recombination analysis were conducted on the Nsp2/ORF5 (ORF5a) genes of the identified strain in the study. The RT-PCR result shown that the positive rate of PRRSV was 50.89% (57/112). Phylogenetic analysis showed that the identified PRRSV strains were all NA genotype and belonged to lineage 1, 3, and 8. The Nsp2 gene of identified PRRSV strains exhibited nucleotide homologies of 53.0 ~ 99.8%, and amino acid homologies of 46.8 ~ 99.7%. The ORF5 gene of identified PRRSV strains exhibited nucleotide homologies of 82.4 ~ 100%, and amino acid homologies of 79.6 ~ 100%. Sequence analysis revealed that a discontinuous 30-amino-acid deletion (positions 481 and 533–561) and a 131-amino-acid discontinuity deletion (positions 323–433, 481, and 533–551) in Nsp2 of PPRSV isolates; all identified strains in this study may be wild strains, and most identified strains may be highly virulent strains. Sequence analysis of ORF5 and ORF5a revealed that the mutation sites of GP5 were mainly concentrated in the signal peptide and epitopes region, while the mutation sites of ORF5a were mainly concentrated in the transmembrane and the intramembrane region. The recombination analysis indicated that there may be multiple recombination regions in identified strains, and the recombination pattern was more complex. This study showed that the prevalent PRRSV strain in some regions of China was still HP-PRRSV, while NADC30 strain also occupied a certain proportion; different types of PRRSV strains showed different patterns and variation in China. This study suggested that the monitoring of PRRSV prevalence and genetic variation should be further strengthened.
format Online
Article
Text
id pubmed-7969665
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-79696652021-03-19 Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018 Yin, Baishuang Qi, Shanshan Sha, Wanli Qin, Hongyu Liu, Liming Yun, Jinyan Zhu, Jinhai Li, Guojiang Sun, Dongbo Front Vet Sci Veterinary Science Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease and brings huge economic losses to commercial pork production worldwide. PRRSV causes severe reproductive failure in sows and respiratory distress in piglets. To trace the evolution of PRRSV in pigs with respiratory diseases in some regions of China, 112 samples were collected from nine provinces in China during 2016–2018. All samples were detected by RT-PCR and analyzed by the Nsp2/ORF5 (ORF5a)-genes-phylogeny. Sequence analysis and recombination analysis were conducted on the Nsp2/ORF5 (ORF5a) genes of the identified strain in the study. The RT-PCR result shown that the positive rate of PRRSV was 50.89% (57/112). Phylogenetic analysis showed that the identified PRRSV strains were all NA genotype and belonged to lineage 1, 3, and 8. The Nsp2 gene of identified PRRSV strains exhibited nucleotide homologies of 53.0 ~ 99.8%, and amino acid homologies of 46.8 ~ 99.7%. The ORF5 gene of identified PRRSV strains exhibited nucleotide homologies of 82.4 ~ 100%, and amino acid homologies of 79.6 ~ 100%. Sequence analysis revealed that a discontinuous 30-amino-acid deletion (positions 481 and 533–561) and a 131-amino-acid discontinuity deletion (positions 323–433, 481, and 533–551) in Nsp2 of PPRSV isolates; all identified strains in this study may be wild strains, and most identified strains may be highly virulent strains. Sequence analysis of ORF5 and ORF5a revealed that the mutation sites of GP5 were mainly concentrated in the signal peptide and epitopes region, while the mutation sites of ORF5a were mainly concentrated in the transmembrane and the intramembrane region. The recombination analysis indicated that there may be multiple recombination regions in identified strains, and the recombination pattern was more complex. This study showed that the prevalent PRRSV strain in some regions of China was still HP-PRRSV, while NADC30 strain also occupied a certain proportion; different types of PRRSV strains showed different patterns and variation in China. This study suggested that the monitoring of PRRSV prevalence and genetic variation should be further strengthened. Frontiers Media S.A. 2021-03-04 /pmc/articles/PMC7969665/ /pubmed/33748205 http://dx.doi.org/10.3389/fvets.2021.605832 Text en Copyright © 2021 Yin, Qi, Sha, Qin, Liu, Yun, Zhu, Li and Sun. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Yin, Baishuang
Qi, Shanshan
Sha, Wanli
Qin, Hongyu
Liu, Liming
Yun, Jinyan
Zhu, Jinhai
Li, Guojiang
Sun, Dongbo
Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018
title Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018
title_full Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018
title_fullStr Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018
title_full_unstemmed Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018
title_short Molecular Characterization of the Nsp2 and ORF5 (ORF5a) Genes of PRRSV Strains in Nine Provinces of China During 2016–2018
title_sort molecular characterization of the nsp2 and orf5 (orf5a) genes of prrsv strains in nine provinces of china during 2016–2018
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7969665/
https://www.ncbi.nlm.nih.gov/pubmed/33748205
http://dx.doi.org/10.3389/fvets.2021.605832
work_keys_str_mv AT yinbaishuang molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT qishanshan molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT shawanli molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT qinhongyu molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT liuliming molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT yunjinyan molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT zhujinhai molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT liguojiang molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018
AT sundongbo molecularcharacterizationofthensp2andorf5orf5agenesofprrsvstrainsinnineprovincesofchinaduring20162018