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Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity

Gonorrhea, a sexually transmitted disease caused by the bacteria Neisseria gonorrhoeae, is characterized by a large number of neutrophils recruited to the site of infection. Therefore, proper modeling of the N. gonorrhoeae interaction with neutrophils is very important for investigating and understa...

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Autores principales: Heydarian, Motaharehsadat, Schweinlin, Matthias, Schwarz, Thomas, Rawal, Ravisha, Walles, Heike, Metzger, Marco, Rudel, Thomas, Kozjak-Pavlovic, Vera
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970704/
https://www.ncbi.nlm.nih.gov/pubmed/33796248
http://dx.doi.org/10.1177/2041731420988802
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author Heydarian, Motaharehsadat
Schweinlin, Matthias
Schwarz, Thomas
Rawal, Ravisha
Walles, Heike
Metzger, Marco
Rudel, Thomas
Kozjak-Pavlovic, Vera
author_facet Heydarian, Motaharehsadat
Schweinlin, Matthias
Schwarz, Thomas
Rawal, Ravisha
Walles, Heike
Metzger, Marco
Rudel, Thomas
Kozjak-Pavlovic, Vera
author_sort Heydarian, Motaharehsadat
collection PubMed
description Gonorrhea, a sexually transmitted disease caused by the bacteria Neisseria gonorrhoeae, is characterized by a large number of neutrophils recruited to the site of infection. Therefore, proper modeling of the N. gonorrhoeae interaction with neutrophils is very important for investigating and understanding the mechanisms that gonococci use to evade the immune response. We have used a combination of a unique human 3D tissue model together with a dynamic culture system to study neutrophil transmigration to the site of N. gonorrhoeae infection. The triple co-culture model consisted of epithelial cells (T84 human colorectal carcinoma cells), human primary dermal fibroblasts, and human umbilical vein endothelial cells on a biological scaffold (SIS). After the infection of the tissue model with N. gonorrhoeae, we introduced primary human neutrophils to the endothelial side of the model using a perfusion-based bioreactor system. By this approach, we were able to demonstrate the activation and transmigration of neutrophils across the 3D tissue model and their recruitment to the site of infection. In summary, the triple co-culture model supplemented by neutrophils represents a promising tool for investigating N. gonorrhoeae and other bacterial infections and interactions with the innate immunity cells under conditions closely resembling the native tissue environment.
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spelling pubmed-79707042021-03-31 Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity Heydarian, Motaharehsadat Schweinlin, Matthias Schwarz, Thomas Rawal, Ravisha Walles, Heike Metzger, Marco Rudel, Thomas Kozjak-Pavlovic, Vera J Tissue Eng Original Article Gonorrhea, a sexually transmitted disease caused by the bacteria Neisseria gonorrhoeae, is characterized by a large number of neutrophils recruited to the site of infection. Therefore, proper modeling of the N. gonorrhoeae interaction with neutrophils is very important for investigating and understanding the mechanisms that gonococci use to evade the immune response. We have used a combination of a unique human 3D tissue model together with a dynamic culture system to study neutrophil transmigration to the site of N. gonorrhoeae infection. The triple co-culture model consisted of epithelial cells (T84 human colorectal carcinoma cells), human primary dermal fibroblasts, and human umbilical vein endothelial cells on a biological scaffold (SIS). After the infection of the tissue model with N. gonorrhoeae, we introduced primary human neutrophils to the endothelial side of the model using a perfusion-based bioreactor system. By this approach, we were able to demonstrate the activation and transmigration of neutrophils across the 3D tissue model and their recruitment to the site of infection. In summary, the triple co-culture model supplemented by neutrophils represents a promising tool for investigating N. gonorrhoeae and other bacterial infections and interactions with the innate immunity cells under conditions closely resembling the native tissue environment. SAGE Publications 2021-01-28 /pmc/articles/PMC7970704/ /pubmed/33796248 http://dx.doi.org/10.1177/2041731420988802 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Heydarian, Motaharehsadat
Schweinlin, Matthias
Schwarz, Thomas
Rawal, Ravisha
Walles, Heike
Metzger, Marco
Rudel, Thomas
Kozjak-Pavlovic, Vera
Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity
title Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity
title_full Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity
title_fullStr Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity
title_full_unstemmed Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity
title_short Triple co-culture and perfusion bioreactor for studying the interaction between Neisseria gonorrhoeae and neutrophils: A novel 3D tissue model for bacterial infection and immunity
title_sort triple co-culture and perfusion bioreactor for studying the interaction between neisseria gonorrhoeae and neutrophils: a novel 3d tissue model for bacterial infection and immunity
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970704/
https://www.ncbi.nlm.nih.gov/pubmed/33796248
http://dx.doi.org/10.1177/2041731420988802
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