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Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture
Polyploids play an important role in the breeding of plant for superior characteristics, and many reports have focused on the effects upon photosynthesis from polyploidization in some plant species recently, yet surprisingly little of this is known for barley. In this study, homozygous diploid and t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970760/ https://www.ncbi.nlm.nih.gov/pubmed/33747007 http://dx.doi.org/10.3389/fpls.2021.626916 |
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author | Chen, Yunyun Xu, Hongwei He, Ting Gao, Runhong Guo, Guimei Lu, Ruiju Chen, Zhiwei Liu, Chenghong |
author_facet | Chen, Yunyun Xu, Hongwei He, Ting Gao, Runhong Guo, Guimei Lu, Ruiju Chen, Zhiwei Liu, Chenghong |
author_sort | Chen, Yunyun |
collection | PubMed |
description | Polyploids play an important role in the breeding of plant for superior characteristics, and many reports have focused on the effects upon photosynthesis from polyploidization in some plant species recently, yet surprisingly little of this is known for barley. In this study, homozygous diploid and tetraploid plants, derived from microspore culturing of the barley cultivar “H30,” were used to assess differences between them in their cellular, photosynthetic, and transcriptomic characteristics. Our results showed that tetraploid barley has the distinct characteristics of polyploids, namely thicker and heavier leaves, enlarged stomata size or stomatal guard cell size, and more photosynthetic pigments and improved photosynthesis (especially under high light intensity). This enhanced photosynthesis of tetraploid barley was confirmed by several photosynthetic parameters, including net photosynthetic rate (P(n)), stomatal conductance (G(s)), intercellular CO(2) concentration (C(i)), transpiration rate (T(r)), maximum net photosynthetic rate (P(max)), light saturation point (LSP), maximum RuBP saturated rate carboxylation (V(cmax)), and maximum rate of electron transport (J(max)). Transcriptomic analyses revealed that just ~2.3% of all detected genes exhibited differential expression patterns [i.e., differentially expressed genes (DEGs)], and that most of these – 580 of 793 DEGs in total – were upregulated in the tetraploid barley. The follow-up KEGG analysis indicated that the most enriched pathway was related to photosynthesis-antenna proteins, while the downregulation of DEGs was related mainly to the light-harvesting cholorophyII a/b-binding protein (Lhcb1) component, both validated by quantitative PCR (qPCR). Taken together, our integrated analysis of morphology, photosynthetic physiology, and transcriptome provides evidences for understanding of how polyploidization enhances the photosynthetic capacity in tetraploids of barley. |
format | Online Article Text |
id | pubmed-7970760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79707602021-03-19 Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture Chen, Yunyun Xu, Hongwei He, Ting Gao, Runhong Guo, Guimei Lu, Ruiju Chen, Zhiwei Liu, Chenghong Front Plant Sci Plant Science Polyploids play an important role in the breeding of plant for superior characteristics, and many reports have focused on the effects upon photosynthesis from polyploidization in some plant species recently, yet surprisingly little of this is known for barley. In this study, homozygous diploid and tetraploid plants, derived from microspore culturing of the barley cultivar “H30,” were used to assess differences between them in their cellular, photosynthetic, and transcriptomic characteristics. Our results showed that tetraploid barley has the distinct characteristics of polyploids, namely thicker and heavier leaves, enlarged stomata size or stomatal guard cell size, and more photosynthetic pigments and improved photosynthesis (especially under high light intensity). This enhanced photosynthesis of tetraploid barley was confirmed by several photosynthetic parameters, including net photosynthetic rate (P(n)), stomatal conductance (G(s)), intercellular CO(2) concentration (C(i)), transpiration rate (T(r)), maximum net photosynthetic rate (P(max)), light saturation point (LSP), maximum RuBP saturated rate carboxylation (V(cmax)), and maximum rate of electron transport (J(max)). Transcriptomic analyses revealed that just ~2.3% of all detected genes exhibited differential expression patterns [i.e., differentially expressed genes (DEGs)], and that most of these – 580 of 793 DEGs in total – were upregulated in the tetraploid barley. The follow-up KEGG analysis indicated that the most enriched pathway was related to photosynthesis-antenna proteins, while the downregulation of DEGs was related mainly to the light-harvesting cholorophyII a/b-binding protein (Lhcb1) component, both validated by quantitative PCR (qPCR). Taken together, our integrated analysis of morphology, photosynthetic physiology, and transcriptome provides evidences for understanding of how polyploidization enhances the photosynthetic capacity in tetraploids of barley. Frontiers Media S.A. 2021-02-26 /pmc/articles/PMC7970760/ /pubmed/33747007 http://dx.doi.org/10.3389/fpls.2021.626916 Text en Copyright © 2021 Chen, Xu, He, Gao, Guo, Lu, Chen and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Chen, Yunyun Xu, Hongwei He, Ting Gao, Runhong Guo, Guimei Lu, Ruiju Chen, Zhiwei Liu, Chenghong Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture |
title | Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture |
title_full | Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture |
title_fullStr | Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture |
title_full_unstemmed | Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture |
title_short | Comparative Analysis of Morphology, Photosynthetic Physiology, and Transcriptome Between Diploid and Tetraploid Barley Derived From Microspore Culture |
title_sort | comparative analysis of morphology, photosynthetic physiology, and transcriptome between diploid and tetraploid barley derived from microspore culture |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970760/ https://www.ncbi.nlm.nih.gov/pubmed/33747007 http://dx.doi.org/10.3389/fpls.2021.626916 |
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