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Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential

Even though the use of antibiotics for food-producing animals may contribute to the emergence of antimicrobial resistance, antibiotics are still used as growth promoters. Due to consumer and regulatory pressures, the use of alternatives to antibiotics as growth promoters is increasing, thus more inf...

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Autores principales: Muurinen, Johanna, Richert, Jacob, Wickware, Carmen L., Richert, Brian, Johnson, Timothy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970892/
https://www.ncbi.nlm.nih.gov/pubmed/33750827
http://dx.doi.org/10.1038/s41598-021-84759-9
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author Muurinen, Johanna
Richert, Jacob
Wickware, Carmen L.
Richert, Brian
Johnson, Timothy A.
author_facet Muurinen, Johanna
Richert, Jacob
Wickware, Carmen L.
Richert, Brian
Johnson, Timothy A.
author_sort Muurinen, Johanna
collection PubMed
description Even though the use of antibiotics for food-producing animals may contribute to the emergence of antimicrobial resistance, antibiotics are still used as growth promoters. Due to consumer and regulatory pressures, the use of alternatives to antibiotics as growth promoters is increasing, thus more information is needed on their capability to disseminate antimicrobial resistance compared to antibiotics. We investigated the impacts of carbadox (antibiotic), copper sulfate and zinc oxide (metals) and mushroom powder (natural product) on the pig fecal resistome and microbiome. Antibiotic resistance gene (ARG) and mobile genetic element (MGE) abundances were measured using a high-throughput qPCR array with 382 primer pairs. Bacterial community composition was determined by 16S rRNA gene sequencing. More ARGs co-occurred with MGEs in the growth promoter group samples than in the control group samples. Community composition could not be linked to resistome in the growth promoter group samples, indicating a potential decoupling of ARGs and phylogeny. Additionally, machine-learning methods aided in defining the community and resistome differences in response to treatments. Since increased ARG mobility potential was the primary response to the dietary additives used in this study, we suggest that ARG mobility should be considered when designing antimicrobial use policies and antimicrobial resistance surveillances.
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spelling pubmed-79708922021-03-19 Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential Muurinen, Johanna Richert, Jacob Wickware, Carmen L. Richert, Brian Johnson, Timothy A. Sci Rep Article Even though the use of antibiotics for food-producing animals may contribute to the emergence of antimicrobial resistance, antibiotics are still used as growth promoters. Due to consumer and regulatory pressures, the use of alternatives to antibiotics as growth promoters is increasing, thus more information is needed on their capability to disseminate antimicrobial resistance compared to antibiotics. We investigated the impacts of carbadox (antibiotic), copper sulfate and zinc oxide (metals) and mushroom powder (natural product) on the pig fecal resistome and microbiome. Antibiotic resistance gene (ARG) and mobile genetic element (MGE) abundances were measured using a high-throughput qPCR array with 382 primer pairs. Bacterial community composition was determined by 16S rRNA gene sequencing. More ARGs co-occurred with MGEs in the growth promoter group samples than in the control group samples. Community composition could not be linked to resistome in the growth promoter group samples, indicating a potential decoupling of ARGs and phylogeny. Additionally, machine-learning methods aided in defining the community and resistome differences in response to treatments. Since increased ARG mobility potential was the primary response to the dietary additives used in this study, we suggest that ARG mobility should be considered when designing antimicrobial use policies and antimicrobial resistance surveillances. Nature Publishing Group UK 2021-03-09 /pmc/articles/PMC7970892/ /pubmed/33750827 http://dx.doi.org/10.1038/s41598-021-84759-9 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Muurinen, Johanna
Richert, Jacob
Wickware, Carmen L.
Richert, Brian
Johnson, Timothy A.
Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_full Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_fullStr Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_full_unstemmed Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_short Swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
title_sort swine growth promotion with antibiotics or alternatives can increase antibiotic resistance gene mobility potential
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7970892/
https://www.ncbi.nlm.nih.gov/pubmed/33750827
http://dx.doi.org/10.1038/s41598-021-84759-9
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