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Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys

The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of...

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Autores principales: de Alcantara, Bianca Nascimento, Imbeloni, Aline Amaral, de Brito Simith Durans, Darlene, de Araújo, Marialva Tereza Ferreira, do Rosário Moutinho da Cruz, Ermelinda, de Carvalho, Carlos Alberto Marques, de Mendonça, Maria Helena Rodrigues, de Sousa, Jorge Rodrigues, Moraes, Adriana Freitas, Filho, Arnaldo Jorge Martins, de Lourdes Gomes Lima, Maria, Neto, Orlando Pereira Amador, Chiang, Jannifer Oliveira, de Azevedo Scalercio, Sarah Raphaella Rocha, Carneiro, Liliane Almeida, Quaresma, Juarez Antônio Simões, da Costa Vasconcelos, Pedro Fernando, de Almeida Medeiros, Daniele Barbosa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971060/
https://www.ncbi.nlm.nih.gov/pubmed/33731800
http://dx.doi.org/10.1038/s41598-021-85571-1
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author de Alcantara, Bianca Nascimento
Imbeloni, Aline Amaral
de Brito Simith Durans, Darlene
de Araújo, Marialva Tereza Ferreira
do Rosário Moutinho da Cruz, Ermelinda
de Carvalho, Carlos Alberto Marques
de Mendonça, Maria Helena Rodrigues
de Sousa, Jorge Rodrigues
Moraes, Adriana Freitas
Filho, Arnaldo Jorge Martins
de Lourdes Gomes Lima, Maria
Neto, Orlando Pereira Amador
Chiang, Jannifer Oliveira
de Azevedo Scalercio, Sarah Raphaella Rocha
Carneiro, Liliane Almeida
Quaresma, Juarez Antônio Simões
da Costa Vasconcelos, Pedro Fernando
de Almeida Medeiros, Daniele Barbosa
author_facet de Alcantara, Bianca Nascimento
Imbeloni, Aline Amaral
de Brito Simith Durans, Darlene
de Araújo, Marialva Tereza Ferreira
do Rosário Moutinho da Cruz, Ermelinda
de Carvalho, Carlos Alberto Marques
de Mendonça, Maria Helena Rodrigues
de Sousa, Jorge Rodrigues
Moraes, Adriana Freitas
Filho, Arnaldo Jorge Martins
de Lourdes Gomes Lima, Maria
Neto, Orlando Pereira Amador
Chiang, Jannifer Oliveira
de Azevedo Scalercio, Sarah Raphaella Rocha
Carneiro, Liliane Almeida
Quaresma, Juarez Antônio Simões
da Costa Vasconcelos, Pedro Fernando
de Almeida Medeiros, Daniele Barbosa
author_sort de Alcantara, Bianca Nascimento
collection PubMed
description The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS.
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spelling pubmed-79710602021-03-19 Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys de Alcantara, Bianca Nascimento Imbeloni, Aline Amaral de Brito Simith Durans, Darlene de Araújo, Marialva Tereza Ferreira do Rosário Moutinho da Cruz, Ermelinda de Carvalho, Carlos Alberto Marques de Mendonça, Maria Helena Rodrigues de Sousa, Jorge Rodrigues Moraes, Adriana Freitas Filho, Arnaldo Jorge Martins de Lourdes Gomes Lima, Maria Neto, Orlando Pereira Amador Chiang, Jannifer Oliveira de Azevedo Scalercio, Sarah Raphaella Rocha Carneiro, Liliane Almeida Quaresma, Juarez Antônio Simões da Costa Vasconcelos, Pedro Fernando de Almeida Medeiros, Daniele Barbosa Sci Rep Article The absence of an adequate animal model for studies has limited the understanding of congenital Zika syndrome (CZS) in humans during the outbreak in America. In this study, we used squirrel monkeys (Saimiri collinsi), a neotropical primate (which mimics the stages of human pregnancy), as a model of Zika virus (ZIKV) infection. Seven pregnant female squirrel monkeys were experimentally infected at three different gestational stages, and we were able reproduce a broad range of clinical manifestations of ZIKV lesions observed in newborn humans. Histopathological and immunohistochemical analyses of early-infected newborns (2/4) revealed damage to various areas of the brain and ZIKV antigens in the cytoplasm of neurons and glial cells, indicative of CZS. The changes caused by ZIKV infection were intrauterine developmental delay, ventriculomegaly, simplified brain gyri, vascular impairment and neuroprogenitor cell dysfunction. Our data show that the ZIKV infection outcome in squirrel monkeys is similar to that in humans, indicating that this model can be used to help answer questions about the effect of ZIKV infection on neuroembryonic development and the morphological changes induced by CZS. Nature Publishing Group UK 2021-03-17 /pmc/articles/PMC7971060/ /pubmed/33731800 http://dx.doi.org/10.1038/s41598-021-85571-1 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
de Alcantara, Bianca Nascimento
Imbeloni, Aline Amaral
de Brito Simith Durans, Darlene
de Araújo, Marialva Tereza Ferreira
do Rosário Moutinho da Cruz, Ermelinda
de Carvalho, Carlos Alberto Marques
de Mendonça, Maria Helena Rodrigues
de Sousa, Jorge Rodrigues
Moraes, Adriana Freitas
Filho, Arnaldo Jorge Martins
de Lourdes Gomes Lima, Maria
Neto, Orlando Pereira Amador
Chiang, Jannifer Oliveira
de Azevedo Scalercio, Sarah Raphaella Rocha
Carneiro, Liliane Almeida
Quaresma, Juarez Antônio Simões
da Costa Vasconcelos, Pedro Fernando
de Almeida Medeiros, Daniele Barbosa
Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_full Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_fullStr Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_full_unstemmed Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_short Histopathological lesions of congenital Zika syndrome in newborn squirrel monkeys
title_sort histopathological lesions of congenital zika syndrome in newborn squirrel monkeys
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971060/
https://www.ncbi.nlm.nih.gov/pubmed/33731800
http://dx.doi.org/10.1038/s41598-021-85571-1
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