Cargando…

Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B

Human adenovirus (HAdV) species B can cause severe acute respiratory diseases. However, the researches to combat this infection have been hampered by the lack of an animal model permissive to the virus. Here, we report in vitro and in vivo HAdV species B infections of tree shrews, the closest relati...

Descripción completa

Detalles Bibliográficos
Autores principales: Li, Xiao, Zhou, Zhichao, Liu, Wenkuan, Fan, Ye, Luo, Yinzhu, Li, Kangtian, Zheng, Zhenxia, Tian, Xingui, Zhou, Rong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971223/
https://www.ncbi.nlm.nih.gov/pubmed/33622191
http://dx.doi.org/10.1080/22221751.2021.1895679
_version_ 1783666572416516096
author Li, Xiao
Zhou, Zhichao
Liu, Wenkuan
Fan, Ye
Luo, Yinzhu
Li, Kangtian
Zheng, Zhenxia
Tian, Xingui
Zhou, Rong
author_facet Li, Xiao
Zhou, Zhichao
Liu, Wenkuan
Fan, Ye
Luo, Yinzhu
Li, Kangtian
Zheng, Zhenxia
Tian, Xingui
Zhou, Rong
author_sort Li, Xiao
collection PubMed
description Human adenovirus (HAdV) species B can cause severe acute respiratory diseases. However, the researches to combat this infection have been hampered by the lack of an animal model permissive to the virus. Here, we report in vitro and in vivo HAdV species B infections of tree shrews, the closest relative of primates. HAdV-3, -7, -14, and -55 efficiently replicated in primary cell cultures. After intranasal inoculation of tree shrews with HAdV-55, the viral replication in the oropharyngeal region remained high until day 5 post-infection and was still detected until day 12. HAdV-55 in the lung or turbinate bone tissues reached the highest levels between days 3 and 5 post-infection, which indicated viral replication in the upper and lower respiratory tracts. HAdV-55 infection caused severe interstitial pneumonia in the animal. IL-8, IL-10, IL-17A, and IFN-γ expression in the peripheral blood mononuclear cells from infected animals was up-regulated. The pre-vaccination with HAdV-55 cleared the virus faster in the respiratory tract, mitigated lung pathological changes. Finally, HAdV-55 infection was propagated among tree shrews. Our study demonstrated that the tree shrew is a permissive animal model for HAdV species B infection and may serve as a valuable platform for testing multiple anti-viral treatments.
format Online
Article
Text
id pubmed-7971223
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Taylor & Francis
record_format MEDLINE/PubMed
spelling pubmed-79712232021-03-31 Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B Li, Xiao Zhou, Zhichao Liu, Wenkuan Fan, Ye Luo, Yinzhu Li, Kangtian Zheng, Zhenxia Tian, Xingui Zhou, Rong Emerg Microbes Infect Research Article Human adenovirus (HAdV) species B can cause severe acute respiratory diseases. However, the researches to combat this infection have been hampered by the lack of an animal model permissive to the virus. Here, we report in vitro and in vivo HAdV species B infections of tree shrews, the closest relative of primates. HAdV-3, -7, -14, and -55 efficiently replicated in primary cell cultures. After intranasal inoculation of tree shrews with HAdV-55, the viral replication in the oropharyngeal region remained high until day 5 post-infection and was still detected until day 12. HAdV-55 in the lung or turbinate bone tissues reached the highest levels between days 3 and 5 post-infection, which indicated viral replication in the upper and lower respiratory tracts. HAdV-55 infection caused severe interstitial pneumonia in the animal. IL-8, IL-10, IL-17A, and IFN-γ expression in the peripheral blood mononuclear cells from infected animals was up-regulated. The pre-vaccination with HAdV-55 cleared the virus faster in the respiratory tract, mitigated lung pathological changes. Finally, HAdV-55 infection was propagated among tree shrews. Our study demonstrated that the tree shrew is a permissive animal model for HAdV species B infection and may serve as a valuable platform for testing multiple anti-viral treatments. Taylor & Francis 2021-03-13 /pmc/articles/PMC7971223/ /pubmed/33622191 http://dx.doi.org/10.1080/22221751.2021.1895679 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Xiao
Zhou, Zhichao
Liu, Wenkuan
Fan, Ye
Luo, Yinzhu
Li, Kangtian
Zheng, Zhenxia
Tian, Xingui
Zhou, Rong
Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
title Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
title_full Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
title_fullStr Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
title_full_unstemmed Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
title_short Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
title_sort chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species b
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971223/
https://www.ncbi.nlm.nih.gov/pubmed/33622191
http://dx.doi.org/10.1080/22221751.2021.1895679
work_keys_str_mv AT lixiao chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT zhouzhichao chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT liuwenkuan chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT fanye chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT luoyinzhu chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT likangtian chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT zhengzhenxia chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT tianxingui chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb
AT zhourong chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb