Cargando…
Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B
Human adenovirus (HAdV) species B can cause severe acute respiratory diseases. However, the researches to combat this infection have been hampered by the lack of an animal model permissive to the virus. Here, we report in vitro and in vivo HAdV species B infections of tree shrews, the closest relati...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971223/ https://www.ncbi.nlm.nih.gov/pubmed/33622191 http://dx.doi.org/10.1080/22221751.2021.1895679 |
_version_ | 1783666572416516096 |
---|---|
author | Li, Xiao Zhou, Zhichao Liu, Wenkuan Fan, Ye Luo, Yinzhu Li, Kangtian Zheng, Zhenxia Tian, Xingui Zhou, Rong |
author_facet | Li, Xiao Zhou, Zhichao Liu, Wenkuan Fan, Ye Luo, Yinzhu Li, Kangtian Zheng, Zhenxia Tian, Xingui Zhou, Rong |
author_sort | Li, Xiao |
collection | PubMed |
description | Human adenovirus (HAdV) species B can cause severe acute respiratory diseases. However, the researches to combat this infection have been hampered by the lack of an animal model permissive to the virus. Here, we report in vitro and in vivo HAdV species B infections of tree shrews, the closest relative of primates. HAdV-3, -7, -14, and -55 efficiently replicated in primary cell cultures. After intranasal inoculation of tree shrews with HAdV-55, the viral replication in the oropharyngeal region remained high until day 5 post-infection and was still detected until day 12. HAdV-55 in the lung or turbinate bone tissues reached the highest levels between days 3 and 5 post-infection, which indicated viral replication in the upper and lower respiratory tracts. HAdV-55 infection caused severe interstitial pneumonia in the animal. IL-8, IL-10, IL-17A, and IFN-γ expression in the peripheral blood mononuclear cells from infected animals was up-regulated. The pre-vaccination with HAdV-55 cleared the virus faster in the respiratory tract, mitigated lung pathological changes. Finally, HAdV-55 infection was propagated among tree shrews. Our study demonstrated that the tree shrew is a permissive animal model for HAdV species B infection and may serve as a valuable platform for testing multiple anti-viral treatments. |
format | Online Article Text |
id | pubmed-7971223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-79712232021-03-31 Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B Li, Xiao Zhou, Zhichao Liu, Wenkuan Fan, Ye Luo, Yinzhu Li, Kangtian Zheng, Zhenxia Tian, Xingui Zhou, Rong Emerg Microbes Infect Research Article Human adenovirus (HAdV) species B can cause severe acute respiratory diseases. However, the researches to combat this infection have been hampered by the lack of an animal model permissive to the virus. Here, we report in vitro and in vivo HAdV species B infections of tree shrews, the closest relative of primates. HAdV-3, -7, -14, and -55 efficiently replicated in primary cell cultures. After intranasal inoculation of tree shrews with HAdV-55, the viral replication in the oropharyngeal region remained high until day 5 post-infection and was still detected until day 12. HAdV-55 in the lung or turbinate bone tissues reached the highest levels between days 3 and 5 post-infection, which indicated viral replication in the upper and lower respiratory tracts. HAdV-55 infection caused severe interstitial pneumonia in the animal. IL-8, IL-10, IL-17A, and IFN-γ expression in the peripheral blood mononuclear cells from infected animals was up-regulated. The pre-vaccination with HAdV-55 cleared the virus faster in the respiratory tract, mitigated lung pathological changes. Finally, HAdV-55 infection was propagated among tree shrews. Our study demonstrated that the tree shrew is a permissive animal model for HAdV species B infection and may serve as a valuable platform for testing multiple anti-viral treatments. Taylor & Francis 2021-03-13 /pmc/articles/PMC7971223/ /pubmed/33622191 http://dx.doi.org/10.1080/22221751.2021.1895679 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Li, Xiao Zhou, Zhichao Liu, Wenkuan Fan, Ye Luo, Yinzhu Li, Kangtian Zheng, Zhenxia Tian, Xingui Zhou, Rong Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B |
title | Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B |
title_full | Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B |
title_fullStr | Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B |
title_full_unstemmed | Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B |
title_short | Chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species B |
title_sort | chinese tree shrew: a permissive model for in vitro and in vivo replication of human adenovirus species b |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971223/ https://www.ncbi.nlm.nih.gov/pubmed/33622191 http://dx.doi.org/10.1080/22221751.2021.1895679 |
work_keys_str_mv | AT lixiao chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT zhouzhichao chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT liuwenkuan chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT fanye chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT luoyinzhu chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT likangtian chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT zhengzhenxia chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT tianxingui chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb AT zhourong chinesetreeshrewapermissivemodelforinvitroandinvivoreplicationofhumanadenovirusspeciesb |