Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci

Background: The Tn916-Tn1545 family of Integrative Conjugative Elements (ICE) are mobile genetic elements (MGEs) that play a role in the spread of antibiotic resistance genes. The Tn916 harbors the tetracycline resistance gene tet(M) and it has been reported in various bacterial species. The increas...

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Autores principales: Lunde, Tracy Munthali, Hjerde, Erik, Al-Haroni, Mohammed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971310/
https://www.ncbi.nlm.nih.gov/pubmed/33796228
http://dx.doi.org/10.1080/20002297.2021.1896874
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author Lunde, Tracy Munthali
Hjerde, Erik
Al-Haroni, Mohammed
author_facet Lunde, Tracy Munthali
Hjerde, Erik
Al-Haroni, Mohammed
author_sort Lunde, Tracy Munthali
collection PubMed
description Background: The Tn916-Tn1545 family of Integrative Conjugative Elements (ICE) are mobile genetic elements (MGEs) that play a role in the spread of antibiotic resistance genes. The Tn916 harbors the tetracycline resistance gene tet(M) and it has been reported in various bacterial species. The increase in the levels of tetracycline resistance among oral streptococci is of great concern primarily due to the abundance of these species in the oral cavity and their ability to act as reservoirs for antibiotic resistance genes.Methods: In the current study, we screened 100 Norwegian clinical oral streptococcal isolates for the presence and diversity of the Tn916-Tn1545 family. In addition, we investigated the transferability the elements, and the associated transfer frequencies.Results: We observed that 21 isolates harboured the Tn916-Tn1545 family and that two of these elements were the novel Tn6815 and Tn6816. The most prevalent member of the Tn916 -Tn1545 family observed in the Norwegian clinical oral streptococcal isolates was the wild type Tn916.Conclusion: The detection of other members of this family of ICE and varying transfer frequencies suggests high versatility of the Tn916 element in oral streptococci in Norway.
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spelling pubmed-79713102021-03-31 Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci Lunde, Tracy Munthali Hjerde, Erik Al-Haroni, Mohammed J Oral Microbiol Original Article Background: The Tn916-Tn1545 family of Integrative Conjugative Elements (ICE) are mobile genetic elements (MGEs) that play a role in the spread of antibiotic resistance genes. The Tn916 harbors the tetracycline resistance gene tet(M) and it has been reported in various bacterial species. The increase in the levels of tetracycline resistance among oral streptococci is of great concern primarily due to the abundance of these species in the oral cavity and their ability to act as reservoirs for antibiotic resistance genes.Methods: In the current study, we screened 100 Norwegian clinical oral streptococcal isolates for the presence and diversity of the Tn916-Tn1545 family. In addition, we investigated the transferability the elements, and the associated transfer frequencies.Results: We observed that 21 isolates harboured the Tn916-Tn1545 family and that two of these elements were the novel Tn6815 and Tn6816. The most prevalent member of the Tn916 -Tn1545 family observed in the Norwegian clinical oral streptococcal isolates was the wild type Tn916.Conclusion: The detection of other members of this family of ICE and varying transfer frequencies suggests high versatility of the Tn916 element in oral streptococci in Norway. Taylor & Francis 2021-03-15 /pmc/articles/PMC7971310/ /pubmed/33796228 http://dx.doi.org/10.1080/20002297.2021.1896874 Text en © 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Lunde, Tracy Munthali
Hjerde, Erik
Al-Haroni, Mohammed
Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci
title Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci
title_full Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci
title_fullStr Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci
title_full_unstemmed Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci
title_short Prevalence, diversity and transferability of the Tn916-Tn1545 family ICE in oral streptococci
title_sort prevalence, diversity and transferability of the tn916-tn1545 family ice in oral streptococci
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971310/
https://www.ncbi.nlm.nih.gov/pubmed/33796228
http://dx.doi.org/10.1080/20002297.2021.1896874
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