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Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples

Collagen is an important component in maintaining structural integrity and functionality of tissues and is modulated in various biological processes. Its visualization and possible quantification using histopathological stains can be important for understanding disease progression or therapeutic res...

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Autores principales: Greiner, Cherry, Grainger, Stephanie, Farrow, Samantha, Davis, Alena, Su, Jimmy L., Saybolt, Matthew D., Wilensky, Robert, Madden, Sean, Sum, Stephen T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971522/
https://www.ncbi.nlm.nih.gov/pubmed/33735190
http://dx.doi.org/10.1371/journal.pone.0248068
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author Greiner, Cherry
Grainger, Stephanie
Farrow, Samantha
Davis, Alena
Su, Jimmy L.
Saybolt, Matthew D.
Wilensky, Robert
Madden, Sean
Sum, Stephen T.
author_facet Greiner, Cherry
Grainger, Stephanie
Farrow, Samantha
Davis, Alena
Su, Jimmy L.
Saybolt, Matthew D.
Wilensky, Robert
Madden, Sean
Sum, Stephen T.
author_sort Greiner, Cherry
collection PubMed
description Collagen is an important component in maintaining structural integrity and functionality of tissues and is modulated in various biological processes. Its visualization and possible quantification using histopathological stains can be important for understanding disease progression or therapeutic response. Visualization of collagen fiber with the histological stain picrosirius red (PSR) is enhanced with polarized light and quantitative analysis is possible using circular polarizers. However, linear polarizers are more commonly available and easier to optically align. The objective of the present study is to demonstrate a novel image acquisition technique and analysis method using linearly polarized light. The proposed imaging technique is based on image acquisition at multiple slide rotation angles, which are co-registered to form a composite image used for quantitative analysis by pixel intensity or pixel counting. The technique was demonstrated on multiple human coronary samples with varying histopathologies and developed specifically to analyze cap collagen in atherosclerotic plaque. Pixel counting image analysis was found to be reproducible across serial tissue sections and across different users and sufficiently sensitive to detect differences in cap structural integrity that are likely relevant to prediction of rupture risk. The benefit of slide rotation angle under linear polarization to acquire images represents a feasible and practical implementation for expanding the general utility of PSR for quantitative analysis.
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spelling pubmed-79715222021-03-31 Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples Greiner, Cherry Grainger, Stephanie Farrow, Samantha Davis, Alena Su, Jimmy L. Saybolt, Matthew D. Wilensky, Robert Madden, Sean Sum, Stephen T. PLoS One Research Article Collagen is an important component in maintaining structural integrity and functionality of tissues and is modulated in various biological processes. Its visualization and possible quantification using histopathological stains can be important for understanding disease progression or therapeutic response. Visualization of collagen fiber with the histological stain picrosirius red (PSR) is enhanced with polarized light and quantitative analysis is possible using circular polarizers. However, linear polarizers are more commonly available and easier to optically align. The objective of the present study is to demonstrate a novel image acquisition technique and analysis method using linearly polarized light. The proposed imaging technique is based on image acquisition at multiple slide rotation angles, which are co-registered to form a composite image used for quantitative analysis by pixel intensity or pixel counting. The technique was demonstrated on multiple human coronary samples with varying histopathologies and developed specifically to analyze cap collagen in atherosclerotic plaque. Pixel counting image analysis was found to be reproducible across serial tissue sections and across different users and sufficiently sensitive to detect differences in cap structural integrity that are likely relevant to prediction of rupture risk. The benefit of slide rotation angle under linear polarization to acquire images represents a feasible and practical implementation for expanding the general utility of PSR for quantitative analysis. Public Library of Science 2021-03-18 /pmc/articles/PMC7971522/ /pubmed/33735190 http://dx.doi.org/10.1371/journal.pone.0248068 Text en © 2021 Greiner et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Greiner, Cherry
Grainger, Stephanie
Farrow, Samantha
Davis, Alena
Su, Jimmy L.
Saybolt, Matthew D.
Wilensky, Robert
Madden, Sean
Sum, Stephen T.
Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
title Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
title_full Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
title_fullStr Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
title_full_unstemmed Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
title_short Robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
title_sort robust quantitative assessment of collagen fibers with picrosirius red stain and linearly polarized light as demonstrated on atherosclerotic plaque samples
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971522/
https://www.ncbi.nlm.nih.gov/pubmed/33735190
http://dx.doi.org/10.1371/journal.pone.0248068
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