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Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study

BACKGROUND: Silicosis is a progressive pneumoconiosis characterized by interstitial fibrosis following exposure to silica dust. The role of metabolic dysregulation in the pathogenesis of silicosis has not been investigated in detail. This study aimed to identify different metabolic features in the p...

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Autores principales: Xue, Changjiang, Wu, Na, Fan, Yali, Ma, Jing, Ye, Qiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971960/
https://www.ncbi.nlm.nih.gov/pubmed/33731064
http://dx.doi.org/10.1186/s12890-021-01462-1
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author Xue, Changjiang
Wu, Na
Fan, Yali
Ma, Jing
Ye, Qiao
author_facet Xue, Changjiang
Wu, Na
Fan, Yali
Ma, Jing
Ye, Qiao
author_sort Xue, Changjiang
collection PubMed
description BACKGROUND: Silicosis is a progressive pneumoconiosis characterized by interstitial fibrosis following exposure to silica dust. The role of metabolic dysregulation in the pathogenesis of silicosis has not been investigated in detail. This study aimed to identify different metabolic features in the plasma of patients with silicosis and dust-exposed workers without silicosis in metabolomics studies. METHODS: Patients with silicosis, dust-exposed workers (DEWs) without silicosis and age-matched healthy controls were recruited in a case–control study. The metabolomics analyses by ultra-high performance liquid chromatography-mass spectrometry were conducted. Distinct metabolic features (DMFs) were identified in the pilot study and were validated in the validation study. The enriched signalling pathways of these DMFs were determined. The ability of DMFs to discriminate among the groups was analysed through receiver operating characteristic (ROC) curves. The correlations between DMFs and clinical features were also explored. RESULTS: Twenty-nine DMFs and 9 DMFs were detected and had the same trend in the pilot study and the validation study in the plasma of the DEW and silicosis groups, respectively. Sphingolipid metabolism was the major metabolic pathway in the DEWs, and arginine and proline metabolism was associated with silicosis. Twenty DMFs in the DEWs and 3 DMFs in the patients with silicosis showed a discriminatory ability with ROC curve analysis. The abundance of kynurenine was higher in Stage III silicosis than in Stage I or Stage II silicosis. l-arginine and kynurenine were both negatively correlated with the percentage of forced vital capacity predicted in silicosis. CONCLUSIONS: Distinct metabolic features in the plasma of DEWs and the patients with silicosis were found to be different. Sphingolipid metabolism and arginine and proline metabolism were identified as the major metabolic pathway in the DEW and silicosis groups, respectively. l-arginine and kynurenine were correlated with the severity of silicosis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12890-021-01462-1.
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spelling pubmed-79719602021-03-19 Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study Xue, Changjiang Wu, Na Fan, Yali Ma, Jing Ye, Qiao BMC Pulm Med Article BACKGROUND: Silicosis is a progressive pneumoconiosis characterized by interstitial fibrosis following exposure to silica dust. The role of metabolic dysregulation in the pathogenesis of silicosis has not been investigated in detail. This study aimed to identify different metabolic features in the plasma of patients with silicosis and dust-exposed workers without silicosis in metabolomics studies. METHODS: Patients with silicosis, dust-exposed workers (DEWs) without silicosis and age-matched healthy controls were recruited in a case–control study. The metabolomics analyses by ultra-high performance liquid chromatography-mass spectrometry were conducted. Distinct metabolic features (DMFs) were identified in the pilot study and were validated in the validation study. The enriched signalling pathways of these DMFs were determined. The ability of DMFs to discriminate among the groups was analysed through receiver operating characteristic (ROC) curves. The correlations between DMFs and clinical features were also explored. RESULTS: Twenty-nine DMFs and 9 DMFs were detected and had the same trend in the pilot study and the validation study in the plasma of the DEW and silicosis groups, respectively. Sphingolipid metabolism was the major metabolic pathway in the DEWs, and arginine and proline metabolism was associated with silicosis. Twenty DMFs in the DEWs and 3 DMFs in the patients with silicosis showed a discriminatory ability with ROC curve analysis. The abundance of kynurenine was higher in Stage III silicosis than in Stage I or Stage II silicosis. l-arginine and kynurenine were both negatively correlated with the percentage of forced vital capacity predicted in silicosis. CONCLUSIONS: Distinct metabolic features in the plasma of DEWs and the patients with silicosis were found to be different. Sphingolipid metabolism and arginine and proline metabolism were identified as the major metabolic pathway in the DEW and silicosis groups, respectively. l-arginine and kynurenine were correlated with the severity of silicosis. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12890-021-01462-1. BioMed Central 2021-03-17 /pmc/articles/PMC7971960/ /pubmed/33731064 http://dx.doi.org/10.1186/s12890-021-01462-1 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Article
Xue, Changjiang
Wu, Na
Fan, Yali
Ma, Jing
Ye, Qiao
Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study
title Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study
title_full Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study
title_fullStr Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study
title_full_unstemmed Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study
title_short Distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in China: a case–control study
title_sort distinct metabolic features in the plasma of patients with silicosis and dust-exposed workers in china: a case–control study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7971960/
https://www.ncbi.nlm.nih.gov/pubmed/33731064
http://dx.doi.org/10.1186/s12890-021-01462-1
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