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Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice

Neurotrophic factors have been implicated in the control of neuronal survival and plasticity in different brain diseases. Meningoencephalitis caused by bovine alpha-herpesvirus 5 (BoHV-5) infection is a frequent neurological disease of young cattle, being the involvement of apoptosis in the developm...

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Autores principales: DA SILVA, Daniele Gonçalves, de CARVALHO, Iracema Luisa Quintino, TOSCANO, Eliana Cristina de Brito, SANTOS, Beatriz Álvares da Silva Senra, OLIVEIRA, Bruna da Silva, CAMPOS, Marco Antônio, da FONSECA, Flávio Guimarães, CAMARGOS, Quezya Mendes, de SOUSA, Gabriela Ferreira, CALIARI, Marcelo Vidigal, TEIXEIRA, Antônio Lúcio, de MIRANDA, Aline Silva, RACHID, Milene Alvarenga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972877/
https://www.ncbi.nlm.nih.gov/pubmed/33281142
http://dx.doi.org/10.1292/jvms.20-0204
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author DA SILVA, Daniele Gonçalves
de CARVALHO, Iracema Luisa Quintino
TOSCANO, Eliana Cristina de Brito
SANTOS, Beatriz Álvares da Silva Senra
OLIVEIRA, Bruna da Silva
CAMPOS, Marco Antônio
da FONSECA, Flávio Guimarães
CAMARGOS, Quezya Mendes
de SOUSA, Gabriela Ferreira
CALIARI, Marcelo Vidigal
TEIXEIRA, Antônio Lúcio
de MIRANDA, Aline Silva
RACHID, Milene Alvarenga
author_facet DA SILVA, Daniele Gonçalves
de CARVALHO, Iracema Luisa Quintino
TOSCANO, Eliana Cristina de Brito
SANTOS, Beatriz Álvares da Silva Senra
OLIVEIRA, Bruna da Silva
CAMPOS, Marco Antônio
da FONSECA, Flávio Guimarães
CAMARGOS, Quezya Mendes
de SOUSA, Gabriela Ferreira
CALIARI, Marcelo Vidigal
TEIXEIRA, Antônio Lúcio
de MIRANDA, Aline Silva
RACHID, Milene Alvarenga
author_sort DA SILVA, Daniele Gonçalves
collection PubMed
description Neurotrophic factors have been implicated in the control of neuronal survival and plasticity in different brain diseases. Meningoencephalitis caused by bovine alpha-herpesvirus 5 (BoHV-5) infection is a frequent neurological disease of young cattle, being the involvement of apoptosis in the development of neuropathological changes frequently discussed in the literature. It’s well known that Toll-like receptors (TLRs) can activate neuroinflammatory response and consequently lead to neuronal loss. However, there are no studies evaluating the expression of neurotrophic factors and their association with brain pathology and TLRs during the infection by BoHV-5. The current study aimed to analyze brain levels of neurotrophic factors along with neuropathological changes during acute infection by BoHV-5 in wild-type (WT) and TLR3/7/9 (TLR3/7/9(−/−)) deficiency mice. The infection was induced by intracranial inoculation of 1 × 10(4) TCID(50) of BoHV-5. Infected animals presented similar degrees of clinical signs and neuropathological changes. Both infected groups had meningoencephalitis and neuronal damage in CA regions from hippocampus. BoHV-5 infection promoted the proliferation of Iba-1 positive cells throughout the neuropil, mainly located in the frontal cortex. Moreover, significant lower levels of brain-derived neurotrophic factor (BDNF) were detected in both BoHV-5 infected WT and TLR3/7/9 deficient mice, compared with non-infected animals. Our study showed that BDNF down regulation was associated with brain inflammation, reactive microgliosis and neuronal loss after bovine alpha-herpesvirus 5 infection in mice. Moreover, we demonstrated that combined TLR3/7/9 deficiency does not alter those parameters.
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spelling pubmed-79728772021-03-23 Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice DA SILVA, Daniele Gonçalves de CARVALHO, Iracema Luisa Quintino TOSCANO, Eliana Cristina de Brito SANTOS, Beatriz Álvares da Silva Senra OLIVEIRA, Bruna da Silva CAMPOS, Marco Antônio da FONSECA, Flávio Guimarães CAMARGOS, Quezya Mendes de SOUSA, Gabriela Ferreira CALIARI, Marcelo Vidigal TEIXEIRA, Antônio Lúcio de MIRANDA, Aline Silva RACHID, Milene Alvarenga J Vet Med Sci Pathology Neurotrophic factors have been implicated in the control of neuronal survival and plasticity in different brain diseases. Meningoencephalitis caused by bovine alpha-herpesvirus 5 (BoHV-5) infection is a frequent neurological disease of young cattle, being the involvement of apoptosis in the development of neuropathological changes frequently discussed in the literature. It’s well known that Toll-like receptors (TLRs) can activate neuroinflammatory response and consequently lead to neuronal loss. However, there are no studies evaluating the expression of neurotrophic factors and their association with brain pathology and TLRs during the infection by BoHV-5. The current study aimed to analyze brain levels of neurotrophic factors along with neuropathological changes during acute infection by BoHV-5 in wild-type (WT) and TLR3/7/9 (TLR3/7/9(−/−)) deficiency mice. The infection was induced by intracranial inoculation of 1 × 10(4) TCID(50) of BoHV-5. Infected animals presented similar degrees of clinical signs and neuropathological changes. Both infected groups had meningoencephalitis and neuronal damage in CA regions from hippocampus. BoHV-5 infection promoted the proliferation of Iba-1 positive cells throughout the neuropil, mainly located in the frontal cortex. Moreover, significant lower levels of brain-derived neurotrophic factor (BDNF) were detected in both BoHV-5 infected WT and TLR3/7/9 deficient mice, compared with non-infected animals. Our study showed that BDNF down regulation was associated with brain inflammation, reactive microgliosis and neuronal loss after bovine alpha-herpesvirus 5 infection in mice. Moreover, we demonstrated that combined TLR3/7/9 deficiency does not alter those parameters. The Japanese Society of Veterinary Science 2020-12-07 2021-02 /pmc/articles/PMC7972877/ /pubmed/33281142 http://dx.doi.org/10.1292/jvms.20-0204 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Pathology
DA SILVA, Daniele Gonçalves
de CARVALHO, Iracema Luisa Quintino
TOSCANO, Eliana Cristina de Brito
SANTOS, Beatriz Álvares da Silva Senra
OLIVEIRA, Bruna da Silva
CAMPOS, Marco Antônio
da FONSECA, Flávio Guimarães
CAMARGOS, Quezya Mendes
de SOUSA, Gabriela Ferreira
CALIARI, Marcelo Vidigal
TEIXEIRA, Antônio Lúcio
de MIRANDA, Aline Silva
RACHID, Milene Alvarenga
Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice
title Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice
title_full Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice
title_fullStr Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice
title_full_unstemmed Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice
title_short Brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and TLR3/7/9 deficient mice
title_sort brain-derived neurotrophic factor is down regulated after bovine alpha-herpesvirus 5 infection in both wild-type and tlr3/7/9 deficient mice
topic Pathology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972877/
https://www.ncbi.nlm.nih.gov/pubmed/33281142
http://dx.doi.org/10.1292/jvms.20-0204
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