Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan

This study aimed to detect filarial parasites in blood samples of Japanese black bears (Ursus thibetanus japonicus) collected from Iwate Prefecture, Japan. Positive amplicons were obtained from 26 out of 30 samples by nested PCR targeting 18S ribosomal RNA gene and first internal transcribed spacer...

Descripción completa

Detalles Bibliográficos
Autores principales: MASATANI, Tatsunori, KOJIMA, Isshu, TASHIRO, Michiyo, YAMAUCHI, Kiyoshi, FUKUI, Daisuke, ICHIKAWA-SEKI, Madoka, HARASAWA, Ryo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Wildlife Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972882/
https://www.ncbi.nlm.nih.gov/pubmed/33311003
http://dx.doi.org/10.1292/jvms.20-0466
_version_ 1783666735550824448
author MASATANI, Tatsunori
KOJIMA, Isshu
TASHIRO, Michiyo
YAMAUCHI, Kiyoshi
FUKUI, Daisuke
ICHIKAWA-SEKI, Madoka
HARASAWA, Ryo
author_facet MASATANI, Tatsunori
KOJIMA, Isshu
TASHIRO, Michiyo
YAMAUCHI, Kiyoshi
FUKUI, Daisuke
ICHIKAWA-SEKI, Madoka
HARASAWA, Ryo
author_sort MASATANI, Tatsunori
collection PubMed
description This study aimed to detect filarial parasites in blood samples of Japanese black bears (Ursus thibetanus japonicus) collected from Iwate Prefecture, Japan. Positive amplicons were obtained from 26 out of 30 samples by nested PCR targeting 18S ribosomal RNA gene and first internal transcribed spacer regions. DNA sequences of Mansonella sp. close to M. ozzardi and Dirofilaria sp. were detected for eight and 11 positive amplicons, respectively. Co-infection was detected for the remaining seven amplicons. Dirofilaria sp. was identified as D. ursi by further genetic analysis of 5S ribosomal RNA gene sequence. The results of this study will contribute to further investigations of Japanese black bears for monitoring their risk as a reservoir of possible zoonotic filarial parasites.
format Online
Article
Text
id pubmed-7972882
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher The Japanese Society of Veterinary Science
record_format MEDLINE/PubMed
spelling pubmed-79728822021-03-23 Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan MASATANI, Tatsunori KOJIMA, Isshu TASHIRO, Michiyo YAMAUCHI, Kiyoshi FUKUI, Daisuke ICHIKAWA-SEKI, Madoka HARASAWA, Ryo J Vet Med Sci Wildlife Science This study aimed to detect filarial parasites in blood samples of Japanese black bears (Ursus thibetanus japonicus) collected from Iwate Prefecture, Japan. Positive amplicons were obtained from 26 out of 30 samples by nested PCR targeting 18S ribosomal RNA gene and first internal transcribed spacer regions. DNA sequences of Mansonella sp. close to M. ozzardi and Dirofilaria sp. were detected for eight and 11 positive amplicons, respectively. Co-infection was detected for the remaining seven amplicons. Dirofilaria sp. was identified as D. ursi by further genetic analysis of 5S ribosomal RNA gene sequence. The results of this study will contribute to further investigations of Japanese black bears for monitoring their risk as a reservoir of possible zoonotic filarial parasites. The Japanese Society of Veterinary Science 2020-12-14 2021-02 /pmc/articles/PMC7972882/ /pubmed/33311003 http://dx.doi.org/10.1292/jvms.20-0466 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Wildlife Science
MASATANI, Tatsunori
KOJIMA, Isshu
TASHIRO, Michiyo
YAMAUCHI, Kiyoshi
FUKUI, Daisuke
ICHIKAWA-SEKI, Madoka
HARASAWA, Ryo
Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan
title Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan
title_full Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan
title_fullStr Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan
title_full_unstemmed Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan
title_short Molecular detection of filarial nematode parasites in Japanese black bears (Ursus thibetanus japonicus) from Iwate Prefecture, Japan
title_sort molecular detection of filarial nematode parasites in japanese black bears (ursus thibetanus japonicus) from iwate prefecture, japan
topic Wildlife Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972882/
https://www.ncbi.nlm.nih.gov/pubmed/33311003
http://dx.doi.org/10.1292/jvms.20-0466
work_keys_str_mv AT masatanitatsunori moleculardetectionoffilarialnematodeparasitesinjapaneseblackbearsursusthibetanusjaponicusfromiwateprefecturejapan
AT kojimaisshu moleculardetectionoffilarialnematodeparasitesinjapaneseblackbearsursusthibetanusjaponicusfromiwateprefecturejapan
AT tashiromichiyo moleculardetectionoffilarialnematodeparasitesinjapaneseblackbearsursusthibetanusjaponicusfromiwateprefecturejapan
AT yamauchikiyoshi moleculardetectionoffilarialnematodeparasitesinjapaneseblackbearsursusthibetanusjaponicusfromiwateprefecturejapan
AT fukuidaisuke moleculardetectionoffilarialnematodeparasitesinjapaneseblackbearsursusthibetanusjaponicusfromiwateprefecturejapan
AT ichikawasekimadoka moleculardetectionoffilarialnematodeparasitesinjapaneseblackbearsursusthibetanusjaponicusfromiwateprefecturejapan
AT harasawaryo moleculardetectionoffilarialnematodeparasitesinjapaneseblackbearsursusthibetanusjaponicusfromiwateprefecturejapan

Ejemplares similares