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The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosis
Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Japanese Society of Veterinary Science
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972883/ https://www.ncbi.nlm.nih.gov/pubmed/33281144 http://dx.doi.org/10.1292/jvms.20-0590 |
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author | SAJIKI, Yamato KONNAI, Satoru NAGATA, Reiko KAWAJI, Satoko NAKAMURA, Hayato FUJISAWA, Sotaro OKAGAWA, Tomohiro MAEKAWA, Naoya KATO, Yukinari SUZUKI, Yasuhiko MURATA, Shiro MORI, Yasuyuki OHASHI, Kazuhiko |
author_facet | SAJIKI, Yamato KONNAI, Satoru NAGATA, Reiko KAWAJI, Satoko NAKAMURA, Hayato FUJISAWA, Sotaro OKAGAWA, Tomohiro MAEKAWA, Naoya KATO, Yukinari SUZUKI, Yasuhiko MURATA, Shiro MORI, Yasuyuki OHASHI, Kazuhiko |
author_sort | SAJIKI, Yamato |
collection | PubMed |
description | Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease. |
format | Online Article Text |
id | pubmed-7972883 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-79728832021-03-23 The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosis SAJIKI, Yamato KONNAI, Satoru NAGATA, Reiko KAWAJI, Satoko NAKAMURA, Hayato FUJISAWA, Sotaro OKAGAWA, Tomohiro MAEKAWA, Naoya KATO, Yukinari SUZUKI, Yasuhiko MURATA, Shiro MORI, Yasuyuki OHASHI, Kazuhiko J Vet Med Sci Immunology Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne’s disease. The Japanese Society of Veterinary Science 2020-12-07 2021-02 /pmc/articles/PMC7972883/ /pubmed/33281144 http://dx.doi.org/10.1292/jvms.20-0590 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Immunology SAJIKI, Yamato KONNAI, Satoru NAGATA, Reiko KAWAJI, Satoko NAKAMURA, Hayato FUJISAWA, Sotaro OKAGAWA, Tomohiro MAEKAWA, Naoya KATO, Yukinari SUZUKI, Yasuhiko MURATA, Shiro MORI, Yasuyuki OHASHI, Kazuhiko The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosis |
title | The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle
infected with Mycobacterium avium subsp.
paratuberculosis |
title_full | The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle
infected with Mycobacterium avium subsp.
paratuberculosis |
title_fullStr | The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle
infected with Mycobacterium avium subsp.
paratuberculosis |
title_full_unstemmed | The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle
infected with Mycobacterium avium subsp.
paratuberculosis |
title_short | The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle
infected with Mycobacterium avium subsp.
paratuberculosis |
title_sort | enhancement of th1 immune response by anti-pd-l1 antibody in cattle
infected with mycobacterium avium subsp.
paratuberculosis |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972883/ https://www.ncbi.nlm.nih.gov/pubmed/33281144 http://dx.doi.org/10.1292/jvms.20-0590 |
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