Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry

The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and dev...

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Autores principales: BISWAS, Peru Gopal, OHARI, Yuma, MOHANTA, Uday Kumar, ITAGAKI, Tadashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Parasitology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972893/
https://www.ncbi.nlm.nih.gov/pubmed/33342971
http://dx.doi.org/10.1292/jvms.20-0664
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author BISWAS, Peru Gopal
OHARI, Yuma
MOHANTA, Uday Kumar
ITAGAKI, Tadashi
author_facet BISWAS, Peru Gopal
OHARI, Yuma
MOHANTA, Uday Kumar
ITAGAKI, Tadashi
author_sort BISWAS, Peru Gopal
collection PubMed
description The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis.
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spelling pubmed-79728932021-03-23 Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry BISWAS, Peru Gopal OHARI, Yuma MOHANTA, Uday Kumar ITAGAKI, Tadashi J Vet Med Sci Parasitology The poultry infections caused by Dispharynx nasuta and Cheilospirura hamulosa nematodes are difficult to be diagnosed by fecal examination because of their egg similarity. In this study, we analyzed DNA sequences of nuclear ribosomal 18S-ITS1-5.8S-ITS2-28S region of D. nasuta and C. hamulosa and developed conventional multiplex PCR method using species-specific primers for discriminating between the two species. The method amplified 455-bp and 319-bp fragments specific to D. nasuta and C. hamulosa, respectively, and did not produce them against the other chicken nematode species, Ascaridia galli, Oxyspirura mansoni, Heterakis gallinarum, Heterakis beramporia, and Heterakis indica, suggesting that the multiplex PCR is sensitive and available for species diagnosis. The Japanese Society of Veterinary Science 2020-12-18 2021-02 /pmc/articles/PMC7972893/ /pubmed/33342971 http://dx.doi.org/10.1292/jvms.20-0664 Text en ©2021 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Parasitology
BISWAS, Peru Gopal
OHARI, Yuma
MOHANTA, Uday Kumar
ITAGAKI, Tadashi
Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry
title Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry
title_full Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry
title_fullStr Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry
title_full_unstemmed Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry
title_short Development of conventional multiplex PCR method for discrimination between Dispharynx nasuta and Cheilospirura hamulosa (Nematoda: Acuariidae) parasitizing poultry
title_sort development of conventional multiplex pcr method for discrimination between dispharynx nasuta and cheilospirura hamulosa (nematoda: acuariidae) parasitizing poultry
topic Parasitology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7972893/
https://www.ncbi.nlm.nih.gov/pubmed/33342971
http://dx.doi.org/10.1292/jvms.20-0664
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