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Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma

Primary gastrointestinal diffuse large B-cell lymphoma (GI-DLBCL) is the most common gastrointestinal lymphoma, but its genetic features are poorly understood. We performed whole-exome sequencing of 25 primary tumor samples from patients with GI-DLBCL and 23 matched normal tissue samples. Oncogenic...

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Autores principales: Li, Peifeng, Chai, Jia, Chen, Zi, Liu, Yang, Wei, Jie, Liu, Yixiong, Zhao, Danhui, Ma, Jing, Wang, Kaijing, Li, Xia, Shao, Yang, Gong, Li, Zhang, Wei, Guo, Shuangping, Yan, Qingguo, Li, Mingyang, Fan, Linni, Wang, Zhe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973209/
https://www.ncbi.nlm.nih.gov/pubmed/33747936
http://dx.doi.org/10.3389/fonc.2021.622648
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author Li, Peifeng
Chai, Jia
Chen, Zi
Liu, Yang
Wei, Jie
Liu, Yixiong
Zhao, Danhui
Ma, Jing
Wang, Kaijing
Li, Xia
Shao, Yang
Gong, Li
Zhang, Wei
Guo, Shuangping
Yan, Qingguo
Li, Mingyang
Fan, Linni
Wang, Zhe
author_facet Li, Peifeng
Chai, Jia
Chen, Zi
Liu, Yang
Wei, Jie
Liu, Yixiong
Zhao, Danhui
Ma, Jing
Wang, Kaijing
Li, Xia
Shao, Yang
Gong, Li
Zhang, Wei
Guo, Shuangping
Yan, Qingguo
Li, Mingyang
Fan, Linni
Wang, Zhe
author_sort Li, Peifeng
collection PubMed
description Primary gastrointestinal diffuse large B-cell lymphoma (GI-DLBCL) is the most common gastrointestinal lymphoma, but its genetic features are poorly understood. We performed whole-exome sequencing of 25 primary tumor samples from patients with GI-DLBCL and 23 matched normal tissue samples. Oncogenic mutations were screened, and the correlations between genetic mutations and clinicopathological characteristics were analyzed. Twenty-five patients with GI-DLBCL were enrolled in the genetic mutation analysis with a median of 184 (range 79–382) protein-altering variants per patient. We identified recurrent oncogenic mutations in GI-DLBCL, including those in TP53, MUC16, B2M, CCND3, HIST1H1C, NEB, and ID3. Compared with nodal DLBCL, GI-DLBCL exhibited an increased mutation frequency of TP53 and reduced mutation frequencies of PIM1, CREBBP, BCL2, KMT2D, and EZH2. Moreover, GI-DLBCL exhibited fewer MYD88 and CD79B mutations than DLBCL in the testis and central nervous system. GI-DLBCLs with HLA-B, MEF2A, RHOA, and NAV3 mutations exhibited a tendency toward a high proliferation index. MUC16 and ETV6 mutations often occurred in tumors with early clinical staging. Our data provide a comprehensive understanding of the landscape of mutations in a small subset of GI-DLBCLs. The genetic mutation profiles of GI-DLBCL differ from those of nodal DLBCL and DLBCL in immune-privileged sites. The different mutated genes are related to the NF-κB and JAK-STAT pathways, and the different pathogenetic mechanisms leading to the development of DLBCL may be influenced by the tissue microenvironment. Differences in genetic alterations might influence the clinicopathological characteristics of GI-DLBCL.
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spelling pubmed-79732092021-03-20 Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma Li, Peifeng Chai, Jia Chen, Zi Liu, Yang Wei, Jie Liu, Yixiong Zhao, Danhui Ma, Jing Wang, Kaijing Li, Xia Shao, Yang Gong, Li Zhang, Wei Guo, Shuangping Yan, Qingguo Li, Mingyang Fan, Linni Wang, Zhe Front Oncol Oncology Primary gastrointestinal diffuse large B-cell lymphoma (GI-DLBCL) is the most common gastrointestinal lymphoma, but its genetic features are poorly understood. We performed whole-exome sequencing of 25 primary tumor samples from patients with GI-DLBCL and 23 matched normal tissue samples. Oncogenic mutations were screened, and the correlations between genetic mutations and clinicopathological characteristics were analyzed. Twenty-five patients with GI-DLBCL were enrolled in the genetic mutation analysis with a median of 184 (range 79–382) protein-altering variants per patient. We identified recurrent oncogenic mutations in GI-DLBCL, including those in TP53, MUC16, B2M, CCND3, HIST1H1C, NEB, and ID3. Compared with nodal DLBCL, GI-DLBCL exhibited an increased mutation frequency of TP53 and reduced mutation frequencies of PIM1, CREBBP, BCL2, KMT2D, and EZH2. Moreover, GI-DLBCL exhibited fewer MYD88 and CD79B mutations than DLBCL in the testis and central nervous system. GI-DLBCLs with HLA-B, MEF2A, RHOA, and NAV3 mutations exhibited a tendency toward a high proliferation index. MUC16 and ETV6 mutations often occurred in tumors with early clinical staging. Our data provide a comprehensive understanding of the landscape of mutations in a small subset of GI-DLBCLs. The genetic mutation profiles of GI-DLBCL differ from those of nodal DLBCL and DLBCL in immune-privileged sites. The different mutated genes are related to the NF-κB and JAK-STAT pathways, and the different pathogenetic mechanisms leading to the development of DLBCL may be influenced by the tissue microenvironment. Differences in genetic alterations might influence the clinicopathological characteristics of GI-DLBCL. Frontiers Media S.A. 2021-03-05 /pmc/articles/PMC7973209/ /pubmed/33747936 http://dx.doi.org/10.3389/fonc.2021.622648 Text en Copyright © 2021 Li, Chai, Chen, Liu, Wei, Liu, Zhao, Ma, Wang, Li, Shao, Gong, Zhang, Guo, Yan, Li, Fan and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Li, Peifeng
Chai, Jia
Chen, Zi
Liu, Yang
Wei, Jie
Liu, Yixiong
Zhao, Danhui
Ma, Jing
Wang, Kaijing
Li, Xia
Shao, Yang
Gong, Li
Zhang, Wei
Guo, Shuangping
Yan, Qingguo
Li, Mingyang
Fan, Linni
Wang, Zhe
Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma
title Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma
title_full Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma
title_fullStr Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma
title_full_unstemmed Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma
title_short Genomic Mutation Profile of Primary Gastrointestinal Diffuse Large B-Cell Lymphoma
title_sort genomic mutation profile of primary gastrointestinal diffuse large b-cell lymphoma
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973209/
https://www.ncbi.nlm.nih.gov/pubmed/33747936
http://dx.doi.org/10.3389/fonc.2021.622648
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