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Practical Approaches for Knock-Out Gene Editing in Pigs
Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973260/ https://www.ncbi.nlm.nih.gov/pubmed/33747029 http://dx.doi.org/10.3389/fgene.2020.617850 |
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author | Ratner, Laura Daniela La Motta, Gaston Emilio Briski, Olinda Salamone, Daniel Felipe Fernandez-Martin, Rafael |
author_facet | Ratner, Laura Daniela La Motta, Gaston Emilio Briski, Olinda Salamone, Daniel Felipe Fernandez-Martin, Rafael |
author_sort | Ratner, Laura Daniela |
collection | PubMed |
description | Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed. |
format | Online Article Text |
id | pubmed-7973260 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79732602021-03-20 Practical Approaches for Knock-Out Gene Editing in Pigs Ratner, Laura Daniela La Motta, Gaston Emilio Briski, Olinda Salamone, Daniel Felipe Fernandez-Martin, Rafael Front Genet Genetics Pigs are an important resource for meat production and serve as a model for human diseases. Due to their physiological and anatomical similarities to humans, these animals can recapitulate symptoms of human diseases, becoming an effective model for biomedical research. Although, in the past pig have not been widely used partially because of the difficulty in genetic modification; nowadays, with the new revolutionary technology of programmable nucleases, and fundamentally of the CRISPR-Cas9 systems, it is possible for the first time to precisely modify the porcine genome as never before. To this purpose, it is necessary to introduce the system into early stage zygotes or to edit cells followed by somatic cell nuclear transfer. In this review, several strategies for pig knock-out gene editing, using the CRISPR-Cas9 system, will be summarized, as well as genotyping methods and different delivery techniques to introduce these tools into the embryos. Finally, the best approaches to produce homogeneous, biallelic edited animals will be discussed. Frontiers Media S.A. 2021-03-05 /pmc/articles/PMC7973260/ /pubmed/33747029 http://dx.doi.org/10.3389/fgene.2020.617850 Text en Copyright © 2021 Ratner, La Motta, Briski, Salamone and Fernandez-Martin. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Ratner, Laura Daniela La Motta, Gaston Emilio Briski, Olinda Salamone, Daniel Felipe Fernandez-Martin, Rafael Practical Approaches for Knock-Out Gene Editing in Pigs |
title | Practical Approaches for Knock-Out Gene Editing in Pigs |
title_full | Practical Approaches for Knock-Out Gene Editing in Pigs |
title_fullStr | Practical Approaches for Knock-Out Gene Editing in Pigs |
title_full_unstemmed | Practical Approaches for Knock-Out Gene Editing in Pigs |
title_short | Practical Approaches for Knock-Out Gene Editing in Pigs |
title_sort | practical approaches for knock-out gene editing in pigs |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973260/ https://www.ncbi.nlm.nih.gov/pubmed/33747029 http://dx.doi.org/10.3389/fgene.2020.617850 |
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