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Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy
Large-scale electron microscopy (EM) allows analysis of both tissues and macromolecules in a semi-automated manner, but acquisition rate forms a bottleneck. We reasoned that a negative bias potential may be used to enhance signal collection, allowing shorter dwell times and thus increasing imaging s...
Autores principales: | Lane, Ryan, Vos, Yoram, Wolters, Anouk H.G., Kessel, Luc van, Chen, S. Elisa, Liv, Nalan, Klumperman, Judith, Giepmans, Ben N.G., Hoogenboom, Jacob P. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973379/ https://www.ncbi.nlm.nih.gov/pubmed/33763642 http://dx.doi.org/10.1016/j.yjsbx.2021.100046 |
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