Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy

Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested. We applied polychromatic polarization microscopy to image th...

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Autores principales: Vitkunaite, Aiste, Laurinaviciene, Aida, Plancoulaine, Benoit, Rasmusson, Allan, Levenson, Richard, Shribak, Michael, Laurinavicius, Arvydas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973427/
https://www.ncbi.nlm.nih.gov/pubmed/33737593
http://dx.doi.org/10.1038/s41598-021-85667-8
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author Vitkunaite, Aiste
Laurinaviciene, Aida
Plancoulaine, Benoit
Rasmusson, Allan
Levenson, Richard
Shribak, Michael
Laurinavicius, Arvydas
author_facet Vitkunaite, Aiste
Laurinaviciene, Aida
Plancoulaine, Benoit
Rasmusson, Allan
Levenson, Richard
Shribak, Michael
Laurinavicius, Arvydas
author_sort Vitkunaite, Aiste
collection PubMed
description Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested. We applied polychromatic polarization microscopy to image their spatial organization. Our study provides evidence that IBI are caused by liquid paraffin-macromolecular crystals formed during paraffin-embedding procedures within cells and potentially reflect an active transcriptional status.
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spelling pubmed-79734272021-03-19 Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy Vitkunaite, Aiste Laurinaviciene, Aida Plancoulaine, Benoit Rasmusson, Allan Levenson, Richard Shribak, Michael Laurinavicius, Arvydas Sci Rep Article Intranuclear birefringent inclusions (IBI) found in various cell types in paraffin-embedded tissue sections have long been considered to be a tissue processing artifact, although an association with biological processes has been suggested. We applied polychromatic polarization microscopy to image their spatial organization. Our study provides evidence that IBI are caused by liquid paraffin-macromolecular crystals formed during paraffin-embedding procedures within cells and potentially reflect an active transcriptional status. Nature Publishing Group UK 2021-03-18 /pmc/articles/PMC7973427/ /pubmed/33737593 http://dx.doi.org/10.1038/s41598-021-85667-8 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Vitkunaite, Aiste
Laurinaviciene, Aida
Plancoulaine, Benoit
Rasmusson, Allan
Levenson, Richard
Shribak, Michael
Laurinavicius, Arvydas
Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
title Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
title_full Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
title_fullStr Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
title_full_unstemmed Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
title_short Intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
title_sort intranuclear birefringent inclusions in paraffin sections by polychromatic polarization microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973427/
https://www.ncbi.nlm.nih.gov/pubmed/33737593
http://dx.doi.org/10.1038/s41598-021-85667-8
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