Cargando…
Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads
Targeted and effective therapy of diseases demands utilization of rapid methods of identification of the given markers. Surface enhanced Raman spectroscopy (SERS) in conjunction with streptavidin–biotin complex is a promising alternative to culture or PCR based methods used for such purposes. Many b...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973519/ https://www.ncbi.nlm.nih.gov/pubmed/33737512 http://dx.doi.org/10.1038/s41598-021-84793-7 |
_version_ | 1783666857807446016 |
---|---|
author | Prucek, Robert Panáček, Aleš Gajdová, Žaneta Večeřová, Renata Kvítek, Libor Gallo, Jiří Kolář, Milan |
author_facet | Prucek, Robert Panáček, Aleš Gajdová, Žaneta Večeřová, Renata Kvítek, Libor Gallo, Jiří Kolář, Milan |
author_sort | Prucek, Robert |
collection | PubMed |
description | Targeted and effective therapy of diseases demands utilization of rapid methods of identification of the given markers. Surface enhanced Raman spectroscopy (SERS) in conjunction with streptavidin–biotin complex is a promising alternative to culture or PCR based methods used for such purposes. Many biotinylated antibodies are available on the market and so this system offers a powerful tool for many analytical applications. Here, we present a very fast and easy-to-use procedure for preparation of streptavidin coated magnetic polystyrene–Au (or Ag) nanocomposite particles as efficient substrate for surface SERS purposes. As a precursor for the preparation of SERS active and magnetically separable composite, commercially available streptavidin coated polystyrene (PS) microparticles with a magnetic core were utilized. These composites of PS particles with silver or gold nanoparticles were prepared by reducing Au(III) or Ag(I) ions using ascorbic acid or dopamine. The choice of the reducing agent influences the morphology and the size of the prepared Ag or Au particles (15–100 nm). The prepare composites were also characterized by HR-TEM images, mapping of elements and also magnetization measurements. The content of Au and Ag was determined by AAS analysis. The synthesized composites have a significantly lower density against magnetic composites based on iron oxides, which considerably decreases the tendency to sedimentation. The polystyrene shell on a magnetic iron oxide core also pronouncedly reduces the inclination to particle aggregation. Moreover, the preparation and purification of this SERS substrate takes only a few minutes. The PS composite with thorny Au particles with the size of approximately 100 nm prepared was utilized for specific and selective detection of Staphylococcus aureus infection in joint knee fluid (PJI) and tau protein (marker for Alzheimer disease). |
format | Online Article Text |
id | pubmed-7973519 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79735192021-03-19 Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads Prucek, Robert Panáček, Aleš Gajdová, Žaneta Večeřová, Renata Kvítek, Libor Gallo, Jiří Kolář, Milan Sci Rep Article Targeted and effective therapy of diseases demands utilization of rapid methods of identification of the given markers. Surface enhanced Raman spectroscopy (SERS) in conjunction with streptavidin–biotin complex is a promising alternative to culture or PCR based methods used for such purposes. Many biotinylated antibodies are available on the market and so this system offers a powerful tool for many analytical applications. Here, we present a very fast and easy-to-use procedure for preparation of streptavidin coated magnetic polystyrene–Au (or Ag) nanocomposite particles as efficient substrate for surface SERS purposes. As a precursor for the preparation of SERS active and magnetically separable composite, commercially available streptavidin coated polystyrene (PS) microparticles with a magnetic core were utilized. These composites of PS particles with silver or gold nanoparticles were prepared by reducing Au(III) or Ag(I) ions using ascorbic acid or dopamine. The choice of the reducing agent influences the morphology and the size of the prepared Ag or Au particles (15–100 nm). The prepare composites were also characterized by HR-TEM images, mapping of elements and also magnetization measurements. The content of Au and Ag was determined by AAS analysis. The synthesized composites have a significantly lower density against magnetic composites based on iron oxides, which considerably decreases the tendency to sedimentation. The polystyrene shell on a magnetic iron oxide core also pronouncedly reduces the inclination to particle aggregation. Moreover, the preparation and purification of this SERS substrate takes only a few minutes. The PS composite with thorny Au particles with the size of approximately 100 nm prepared was utilized for specific and selective detection of Staphylococcus aureus infection in joint knee fluid (PJI) and tau protein (marker for Alzheimer disease). Nature Publishing Group UK 2021-03-18 /pmc/articles/PMC7973519/ /pubmed/33737512 http://dx.doi.org/10.1038/s41598-021-84793-7 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Prucek, Robert Panáček, Aleš Gajdová, Žaneta Večeřová, Renata Kvítek, Libor Gallo, Jiří Kolář, Milan Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads |
title | Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads |
title_full | Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads |
title_fullStr | Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads |
title_full_unstemmed | Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads |
title_short | Specific detection of Staphylococcus aureus infection and marker for Alzheimer disease by surface enhanced Raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads |
title_sort | specific detection of staphylococcus aureus infection and marker for alzheimer disease by surface enhanced raman spectroscopy using silver and gold nanoparticle-coated magnetic polystyrene beads |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973519/ https://www.ncbi.nlm.nih.gov/pubmed/33737512 http://dx.doi.org/10.1038/s41598-021-84793-7 |
work_keys_str_mv | AT prucekrobert specificdetectionofstaphylococcusaureusinfectionandmarkerforalzheimerdiseasebysurfaceenhancedramanspectroscopyusingsilverandgoldnanoparticlecoatedmagneticpolystyrenebeads AT panacekales specificdetectionofstaphylococcusaureusinfectionandmarkerforalzheimerdiseasebysurfaceenhancedramanspectroscopyusingsilverandgoldnanoparticlecoatedmagneticpolystyrenebeads AT gajdovazaneta specificdetectionofstaphylococcusaureusinfectionandmarkerforalzheimerdiseasebysurfaceenhancedramanspectroscopyusingsilverandgoldnanoparticlecoatedmagneticpolystyrenebeads AT vecerovarenata specificdetectionofstaphylococcusaureusinfectionandmarkerforalzheimerdiseasebysurfaceenhancedramanspectroscopyusingsilverandgoldnanoparticlecoatedmagneticpolystyrenebeads AT kviteklibor specificdetectionofstaphylococcusaureusinfectionandmarkerforalzheimerdiseasebysurfaceenhancedramanspectroscopyusingsilverandgoldnanoparticlecoatedmagneticpolystyrenebeads AT gallojiri specificdetectionofstaphylococcusaureusinfectionandmarkerforalzheimerdiseasebysurfaceenhancedramanspectroscopyusingsilverandgoldnanoparticlecoatedmagneticpolystyrenebeads AT kolarmilan specificdetectionofstaphylococcusaureusinfectionandmarkerforalzheimerdiseasebysurfaceenhancedramanspectroscopyusingsilverandgoldnanoparticlecoatedmagneticpolystyrenebeads |