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Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits
In plants, Fruits and their wastes are the main sources of bioactive compounds. Currently, Annona fruits have attracted the attention of people interested in health-promoting foods due to their phytochemical content that their activities were not studied before. This study aimed to explore the poten...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973736/ https://www.ncbi.nlm.nih.gov/pubmed/33737634 http://dx.doi.org/10.1038/s41598-021-85772-8 |
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author | Shehata, Mohamed Gamal Abu-Serie, Marwa Muhammad Abd El-Aziz, Nourhan Mohammad El-Sohaimy, Sobhy Ahmed |
author_facet | Shehata, Mohamed Gamal Abu-Serie, Marwa Muhammad Abd El-Aziz, Nourhan Mohammad El-Sohaimy, Sobhy Ahmed |
author_sort | Shehata, Mohamed Gamal |
collection | PubMed |
description | In plants, Fruits and their wastes are the main sources of bioactive compounds. Currently, Annona fruits have attracted the attention of people interested in health-promoting foods due to their phytochemical content that their activities were not studied before. This study aimed to explore the potential antioxidant, antimicrobial, and in vitro anticancer activity of two cultivars Annona squamosa (Annona b. and Annona h.) seed, peel, and pulp. We also meausred phenolic, flavonoid, sulfated polysaccharide, tannins, and triterpenoids. Polyphenol identification was determined using RP-HPLC. Results of the antioxidant activity revealed that the highest activity was observed for Annona h. seed extract using DPPH and ABTS assays with IC(50) 6.07 ± 0.50 and 9.58 ± 0.53 µg/ml, respectively. The antimicrobial activity against various pathogenic strains revealed that the peel extracts of both Annona b. and Annona h. exhibited the best antimicrobial activity. We also assessed the IC(50) values for anticancer activity in all six Annona b. and Annona h samples against four cancer cell lines colon (Caco-2), prostate (PC3), liver (HepG-2), and breast (MCF-7) using MTT assay. Annona b. and Annona h seed extracts had the lowest IC(50) values for four cancer cell lines with 7.31 ± 0.03 and 15.99 ± 1.25 for PC-3 and MCF-7, respectively. Both seed extracts, Annona b. and Annona h., showed significantly down-regulated mRNA expression of Bcl-2 and up-regulated p53 in all treated cell lines. Apoptosis was evaluated using nuclear staining, flow cytometric analysis, and immunohistochemistry of the proliferation marker (Ki-67). Additional studies are required to characterize the bioactive compounds responsible for the observed activities of Annona seed and determine its mechanism as an anticancer drug. |
format | Online Article Text |
id | pubmed-7973736 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79737362021-03-19 Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits Shehata, Mohamed Gamal Abu-Serie, Marwa Muhammad Abd El-Aziz, Nourhan Mohammad El-Sohaimy, Sobhy Ahmed Sci Rep Article In plants, Fruits and their wastes are the main sources of bioactive compounds. Currently, Annona fruits have attracted the attention of people interested in health-promoting foods due to their phytochemical content that their activities were not studied before. This study aimed to explore the potential antioxidant, antimicrobial, and in vitro anticancer activity of two cultivars Annona squamosa (Annona b. and Annona h.) seed, peel, and pulp. We also meausred phenolic, flavonoid, sulfated polysaccharide, tannins, and triterpenoids. Polyphenol identification was determined using RP-HPLC. Results of the antioxidant activity revealed that the highest activity was observed for Annona h. seed extract using DPPH and ABTS assays with IC(50) 6.07 ± 0.50 and 9.58 ± 0.53 µg/ml, respectively. The antimicrobial activity against various pathogenic strains revealed that the peel extracts of both Annona b. and Annona h. exhibited the best antimicrobial activity. We also assessed the IC(50) values for anticancer activity in all six Annona b. and Annona h samples against four cancer cell lines colon (Caco-2), prostate (PC3), liver (HepG-2), and breast (MCF-7) using MTT assay. Annona b. and Annona h seed extracts had the lowest IC(50) values for four cancer cell lines with 7.31 ± 0.03 and 15.99 ± 1.25 for PC-3 and MCF-7, respectively. Both seed extracts, Annona b. and Annona h., showed significantly down-regulated mRNA expression of Bcl-2 and up-regulated p53 in all treated cell lines. Apoptosis was evaluated using nuclear staining, flow cytometric analysis, and immunohistochemistry of the proliferation marker (Ki-67). Additional studies are required to characterize the bioactive compounds responsible for the observed activities of Annona seed and determine its mechanism as an anticancer drug. Nature Publishing Group UK 2021-03-18 /pmc/articles/PMC7973736/ /pubmed/33737634 http://dx.doi.org/10.1038/s41598-021-85772-8 Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Shehata, Mohamed Gamal Abu-Serie, Marwa Muhammad Abd El-Aziz, Nourhan Mohammad El-Sohaimy, Sobhy Ahmed Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits |
title | Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits |
title_full | Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits |
title_fullStr | Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits |
title_full_unstemmed | Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits |
title_short | Nutritional, phytochemical, and in vitro anticancer potential of sugar apple (Annona squamosa) fruits |
title_sort | nutritional, phytochemical, and in vitro anticancer potential of sugar apple (annona squamosa) fruits |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7973736/ https://www.ncbi.nlm.nih.gov/pubmed/33737634 http://dx.doi.org/10.1038/s41598-021-85772-8 |
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