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miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis
Endometriosis (EM) is a multifactorial and debilitating chronic benign gynecological disease, but the pathogenesis of the disease is not completely understood. Dysregulated expression of microRNAs (miRNA/miR) is associated with the etiology of EM due to their role in regulating endometrial stromal c...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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D.A. Spandidos
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974257/ https://www.ncbi.nlm.nih.gov/pubmed/33760149 http://dx.doi.org/10.3892/mmr.2021.11995 |
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author | Yang, Hong Hu, Tianqi Hu, Panwei Qi, Cong Qian, Lin |
author_facet | Yang, Hong Hu, Tianqi Hu, Panwei Qi, Cong Qian, Lin |
author_sort | Yang, Hong |
collection | PubMed |
description | Endometriosis (EM) is a multifactorial and debilitating chronic benign gynecological disease, but the pathogenesis of the disease is not completely understood. Dysregulated expression of microRNAs (miRNA/miR) is associated with the etiology of EM due to their role in regulating endometrial stromal cell proliferation and invasion. The present study aimed to identify the functions and mechanisms underlying miR-143-3p in EM. To explore the role of miR-143-3p in EM, functional miRNAs were analyzed via bioinformatics analysis. miR-143-3p expression levels in endometriotic stromal cells (ESCs) and normal endometrial stromal cells (NESCs) were measured via reverse transcription-quantitative PCR. The role of miR-143-3p in regulating ESC proliferation and invasion was assessed by performing Cell Counting Kit-8 and Transwell assays, respectively. miR-143-3p expression was significantly upregulated in ESCs compared with NESCs. Functionally, miR-143-3p overexpression inhibited ESC proliferation and invasion, whereas miR-143-3p knockdown promoted ESC proliferation and invasion. Moreover, miR-143-3p inhibited autophagy activation in ESCs, as indicated by decreased green puncta, which represented autophagic vacuoles, decreased microtubule associated protein 1 light chain 3α expression and increased p62 expression in the miR-143-4p mimic group compared with the control group. Moreover, compared with the control group, miR-143-3p overexpression significantly decreased the expression levels of autophagy-related 2B (ATG2B), a newly identified target gene of miR-143-3p, in ESCs. ATG2B overexpression reversed miR-143-3p overexpression-mediated inhibition of ESC proliferation and invasion. Collectively, the results of the present study suggested that miR-143-3p inhibited EM progression, thus providing a novel target for the development of therapeutic agents against EM. |
format | Online Article Text |
id | pubmed-7974257 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-79742572021-03-24 miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis Yang, Hong Hu, Tianqi Hu, Panwei Qi, Cong Qian, Lin Mol Med Rep Articles Endometriosis (EM) is a multifactorial and debilitating chronic benign gynecological disease, but the pathogenesis of the disease is not completely understood. Dysregulated expression of microRNAs (miRNA/miR) is associated with the etiology of EM due to their role in regulating endometrial stromal cell proliferation and invasion. The present study aimed to identify the functions and mechanisms underlying miR-143-3p in EM. To explore the role of miR-143-3p in EM, functional miRNAs were analyzed via bioinformatics analysis. miR-143-3p expression levels in endometriotic stromal cells (ESCs) and normal endometrial stromal cells (NESCs) were measured via reverse transcription-quantitative PCR. The role of miR-143-3p in regulating ESC proliferation and invasion was assessed by performing Cell Counting Kit-8 and Transwell assays, respectively. miR-143-3p expression was significantly upregulated in ESCs compared with NESCs. Functionally, miR-143-3p overexpression inhibited ESC proliferation and invasion, whereas miR-143-3p knockdown promoted ESC proliferation and invasion. Moreover, miR-143-3p inhibited autophagy activation in ESCs, as indicated by decreased green puncta, which represented autophagic vacuoles, decreased microtubule associated protein 1 light chain 3α expression and increased p62 expression in the miR-143-4p mimic group compared with the control group. Moreover, compared with the control group, miR-143-3p overexpression significantly decreased the expression levels of autophagy-related 2B (ATG2B), a newly identified target gene of miR-143-3p, in ESCs. ATG2B overexpression reversed miR-143-3p overexpression-mediated inhibition of ESC proliferation and invasion. Collectively, the results of the present study suggested that miR-143-3p inhibited EM progression, thus providing a novel target for the development of therapeutic agents against EM. D.A. Spandidos 2021-05 2021-03-12 /pmc/articles/PMC7974257/ /pubmed/33760149 http://dx.doi.org/10.3892/mmr.2021.11995 Text en Copyright: © Yang et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Yang, Hong Hu, Tianqi Hu, Panwei Qi, Cong Qian, Lin miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis |
title | miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis |
title_full | miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis |
title_fullStr | miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis |
title_full_unstemmed | miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis |
title_short | miR-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis |
title_sort | mir-143-3p inhibits endometriotic stromal cell proliferation and invasion by inactivating autophagy in endometriosis |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7974257/ https://www.ncbi.nlm.nih.gov/pubmed/33760149 http://dx.doi.org/10.3892/mmr.2021.11995 |
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