Cargando…
Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles
Background: Post-contrast acute kidney injury (PC-AKI) is a severe complication of cardiac catheterization. Emerging evidence indicated that long non-coding RNAs (lncRNAs) could serve as biomarkers for various diseases. However, the lncRNA expression profile and potential biomarkers in PC-AKI remain...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975710/ https://www.ncbi.nlm.nih.gov/pubmed/33767596 http://dx.doi.org/10.7150/ijbs.45294 |
_version_ | 1783666969838354432 |
---|---|
author | Chen, Guanzhong Liu, Bowen Chen, Shiqun Li, Huanqiang Liu, Jin Mai, Ziling Chen, Enzhao Zhou, Chunyun Sun, Guoli Guo, Zhaodong Lei, Li Huang, Shanyi Zhang, Liyao Li, Min Tan, Ning Li, Hong Liao, Yulin Liu, Jia Chen, Jiyan Liu, Yong |
author_facet | Chen, Guanzhong Liu, Bowen Chen, Shiqun Li, Huanqiang Liu, Jin Mai, Ziling Chen, Enzhao Zhou, Chunyun Sun, Guoli Guo, Zhaodong Lei, Li Huang, Shanyi Zhang, Liyao Li, Min Tan, Ning Li, Hong Liao, Yulin Liu, Jia Chen, Jiyan Liu, Yong |
author_sort | Chen, Guanzhong |
collection | PubMed |
description | Background: Post-contrast acute kidney injury (PC-AKI) is a severe complication of cardiac catheterization. Emerging evidence indicated that long non-coding RNAs (lncRNAs) could serve as biomarkers for various diseases. However, the lncRNA expression profile and potential biomarkers in PC-AKI remain unclear. This study aimed to investigate novel lncRNA biomarkers for the early detection of PC-AKI. Methods: lncRNA profile in the kidney tissues of PC-AKI rats was evaluated through RNA sequencing. Potential lncRNA biomarkers were identified through human-rat homology analysis, kidney and blood filtering in rats and verified in 112 clinical samples. The expression patterns of the candidate lncRNAs were detected in HK-2 cells and rat models to evaluate their potential for early detection. Results: In total, 357 lncRNAs were found to be differentially expressed in PC-AKI. We identified lnc-HILPDA and lnc-PRND were conservative and remarkably upregulated in both kidneys and blood from rats and the blood of PC-AKI patients; these lncRNAs can precisely distinguish PC-AKI patients (area under the curve (AUC) values of 0.885 and 0.875, respectively). The combination of these two lncRNAs exhibited improved accuracy for predicting PC-AKI, with 100% sensitivity and 83.93% specificity. Time-course experiments showed that the significant difference was first noted in the blood of PC-AKI rats at 12 h for lnc-HILPDA and 24 h for lnc-PRND. Conclusion: Our study revealed that lnc-HILPDA and lnc-PRND may serve as the novel biomarkers for early detection and profoundly affect the clinical stratification and strategy guidance of PC-AKI. |
format | Online Article Text |
id | pubmed-7975710 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-79757102021-03-24 Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles Chen, Guanzhong Liu, Bowen Chen, Shiqun Li, Huanqiang Liu, Jin Mai, Ziling Chen, Enzhao Zhou, Chunyun Sun, Guoli Guo, Zhaodong Lei, Li Huang, Shanyi Zhang, Liyao Li, Min Tan, Ning Li, Hong Liao, Yulin Liu, Jia Chen, Jiyan Liu, Yong Int J Biol Sci Research Paper Background: Post-contrast acute kidney injury (PC-AKI) is a severe complication of cardiac catheterization. Emerging evidence indicated that long non-coding RNAs (lncRNAs) could serve as biomarkers for various diseases. However, the lncRNA expression profile and potential biomarkers in PC-AKI remain unclear. This study aimed to investigate novel lncRNA biomarkers for the early detection of PC-AKI. Methods: lncRNA profile in the kidney tissues of PC-AKI rats was evaluated through RNA sequencing. Potential lncRNA biomarkers were identified through human-rat homology analysis, kidney and blood filtering in rats and verified in 112 clinical samples. The expression patterns of the candidate lncRNAs were detected in HK-2 cells and rat models to evaluate their potential for early detection. Results: In total, 357 lncRNAs were found to be differentially expressed in PC-AKI. We identified lnc-HILPDA and lnc-PRND were conservative and remarkably upregulated in both kidneys and blood from rats and the blood of PC-AKI patients; these lncRNAs can precisely distinguish PC-AKI patients (area under the curve (AUC) values of 0.885 and 0.875, respectively). The combination of these two lncRNAs exhibited improved accuracy for predicting PC-AKI, with 100% sensitivity and 83.93% specificity. Time-course experiments showed that the significant difference was first noted in the blood of PC-AKI rats at 12 h for lnc-HILPDA and 24 h for lnc-PRND. Conclusion: Our study revealed that lnc-HILPDA and lnc-PRND may serve as the novel biomarkers for early detection and profoundly affect the clinical stratification and strategy guidance of PC-AKI. Ivyspring International Publisher 2021-02-17 /pmc/articles/PMC7975710/ /pubmed/33767596 http://dx.doi.org/10.7150/ijbs.45294 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Chen, Guanzhong Liu, Bowen Chen, Shiqun Li, Huanqiang Liu, Jin Mai, Ziling Chen, Enzhao Zhou, Chunyun Sun, Guoli Guo, Zhaodong Lei, Li Huang, Shanyi Zhang, Liyao Li, Min Tan, Ning Li, Hong Liao, Yulin Liu, Jia Chen, Jiyan Liu, Yong Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles |
title | Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles |
title_full | Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles |
title_fullStr | Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles |
title_full_unstemmed | Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles |
title_short | Novel biomarkers for post-contrast acute kidney injury identified from long non-coding RNA expression profiles |
title_sort | novel biomarkers for post-contrast acute kidney injury identified from long non-coding rna expression profiles |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7975710/ https://www.ncbi.nlm.nih.gov/pubmed/33767596 http://dx.doi.org/10.7150/ijbs.45294 |
work_keys_str_mv | AT chenguanzhong novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT liubowen novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT chenshiqun novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT lihuanqiang novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT liujin novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT maiziling novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT chenenzhao novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT zhouchunyun novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT sunguoli novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT guozhaodong novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT leili novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT huangshanyi novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT zhangliyao novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT limin novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT tanning novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT lihong novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT liaoyulin novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT liujia novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT chenjiyan novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles AT liuyong novelbiomarkersforpostcontrastacutekidneyinjuryidentifiedfromlongnoncodingrnaexpressionprofiles |