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Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification
Dual emissions at ~700 and 800 nm have been achieved from a single NIR-AZA fluorophore 1 by establishing parameters in which it can exist in either its isolated molecular or aggregated states. Dual near infrared (NIR) fluorescence color lymph node (LN) mapping with 1 was achieved in a large-animal p...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976573/ https://www.ncbi.nlm.nih.gov/pubmed/33746570 http://dx.doi.org/10.7150/ijms.52816 |
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author | Curtin, Niamh Wu, Dan Cahill, Ronan Sarkar, Anwesha Aonghusa, Pól Mac Zhuk, Sergiy Barberio, Manuel Al-Taher, Mahdi Marescaux, Jacques Diana, Michele O'Shea, Donal F. |
author_facet | Curtin, Niamh Wu, Dan Cahill, Ronan Sarkar, Anwesha Aonghusa, Pól Mac Zhuk, Sergiy Barberio, Manuel Al-Taher, Mahdi Marescaux, Jacques Diana, Michele O'Shea, Donal F. |
author_sort | Curtin, Niamh |
collection | PubMed |
description | Dual emissions at ~700 and 800 nm have been achieved from a single NIR-AZA fluorophore 1 by establishing parameters in which it can exist in either its isolated molecular or aggregated states. Dual near infrared (NIR) fluorescence color lymph node (LN) mapping with 1 was achieved in a large-animal porcine model, with injection site, channels and nodes all detectable at both 700 and 800 nm using a preclinical open camera system. The fluorophore was also compatible with imaging using two clinical instruments for fluorescence guided surgery. Methods: An NIR-AZA fluorophore with hydrophilic and phobic features was synthesised in a straightforward manner and its aggregation properties characterised spectroscopically and by TEM imaging. Toxicity was assessed in a rodent model and dual color fluorescence imaging evaluated by lymph node mapping in a large animal porcine models and in ex-vivo human tissue specimen. Results: Dual color fluorescence imaging has been achieved in the highly complex biomedical scenario of lymph node mapping. Emissions at 700 and 800 nm can be achieved from a single fluorophore by establishing molecular and aggregate forms. Fluorophore was compatible with clinical systems for fluorescence guided surgery and no toxicity was observed in high dosage testing. Conclusion: A new, biomedical compatible form of NIR-dual emission wavelength imaging has been established using a readily accessible fluorophore with significant scope for clinical translation. |
format | Online Article Text |
id | pubmed-7976573 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-79765732021-03-19 Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification Curtin, Niamh Wu, Dan Cahill, Ronan Sarkar, Anwesha Aonghusa, Pól Mac Zhuk, Sergiy Barberio, Manuel Al-Taher, Mahdi Marescaux, Jacques Diana, Michele O'Shea, Donal F. Int J Med Sci Research Paper Dual emissions at ~700 and 800 nm have been achieved from a single NIR-AZA fluorophore 1 by establishing parameters in which it can exist in either its isolated molecular or aggregated states. Dual near infrared (NIR) fluorescence color lymph node (LN) mapping with 1 was achieved in a large-animal porcine model, with injection site, channels and nodes all detectable at both 700 and 800 nm using a preclinical open camera system. The fluorophore was also compatible with imaging using two clinical instruments for fluorescence guided surgery. Methods: An NIR-AZA fluorophore with hydrophilic and phobic features was synthesised in a straightforward manner and its aggregation properties characterised spectroscopically and by TEM imaging. Toxicity was assessed in a rodent model and dual color fluorescence imaging evaluated by lymph node mapping in a large animal porcine models and in ex-vivo human tissue specimen. Results: Dual color fluorescence imaging has been achieved in the highly complex biomedical scenario of lymph node mapping. Emissions at 700 and 800 nm can be achieved from a single fluorophore by establishing molecular and aggregate forms. Fluorophore was compatible with clinical systems for fluorescence guided surgery and no toxicity was observed in high dosage testing. Conclusion: A new, biomedical compatible form of NIR-dual emission wavelength imaging has been established using a readily accessible fluorophore with significant scope for clinical translation. Ivyspring International Publisher 2021-02-03 /pmc/articles/PMC7976573/ /pubmed/33746570 http://dx.doi.org/10.7150/ijms.52816 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Curtin, Niamh Wu, Dan Cahill, Ronan Sarkar, Anwesha Aonghusa, Pól Mac Zhuk, Sergiy Barberio, Manuel Al-Taher, Mahdi Marescaux, Jacques Diana, Michele O'Shea, Donal F. Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification |
title | Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification |
title_full | Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification |
title_fullStr | Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification |
title_full_unstemmed | Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification |
title_short | Dual Color Imaging from a Single BF(2)-Azadipyrromethene Fluorophore Demonstrated in vivo for Lymph Node Identification |
title_sort | dual color imaging from a single bf(2)-azadipyrromethene fluorophore demonstrated in vivo for lymph node identification |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7976573/ https://www.ncbi.nlm.nih.gov/pubmed/33746570 http://dx.doi.org/10.7150/ijms.52816 |
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