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10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales

Osteoporosis is the most common aging-associated bone disease and is caused by hyperactivation of osteoclastic activity. We previously reported that the hexane extract of ginger rhizome [ginger hexane extract (GHE)] could suppress receptor activator of nuclear factor kappa-B ligand (RANKL)-induced o...

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Autores principales: Zang, Liqing, Kagotani, Kazuhiro, Nakayama, Hiroko, Bhagat, Jacky, Fujimoto, Yuki, Hayashi, Akihito, Sono, Ryoji, Katsuzaki, Hirotaka, Nishimura, Norihiro, Shimada, Yasuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978033/
https://www.ncbi.nlm.nih.gov/pubmed/33748100
http://dx.doi.org/10.3389/fcell.2021.588093
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author Zang, Liqing
Kagotani, Kazuhiro
Nakayama, Hiroko
Bhagat, Jacky
Fujimoto, Yuki
Hayashi, Akihito
Sono, Ryoji
Katsuzaki, Hirotaka
Nishimura, Norihiro
Shimada, Yasuhito
author_facet Zang, Liqing
Kagotani, Kazuhiro
Nakayama, Hiroko
Bhagat, Jacky
Fujimoto, Yuki
Hayashi, Akihito
Sono, Ryoji
Katsuzaki, Hirotaka
Nishimura, Norihiro
Shimada, Yasuhito
author_sort Zang, Liqing
collection PubMed
description Osteoporosis is the most common aging-associated bone disease and is caused by hyperactivation of osteoclastic activity. We previously reported that the hexane extract of ginger rhizome [ginger hexane extract (GHE)] could suppress receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. However, the anti-osteoclastic components in GHE have not yet been identified. In this study, we separated GHE into several fractions using silica gel column chromatography and evaluated their effects on osteoclastogenesis using a RAW264.7 cell osteoclast differentiation assay (in vitro) and the zebrafish scale model of osteoporosis (in vivo). We identified that the fractions containing 10-gingerol suppressed osteoclastogenesis in RAW264.7 cells detected by tartrate-resistant acid phosphatase (TRAP) staining. In zebrafish, GHE and 10-gingerol suppressed osteoclastogenesis in prednisolone-induced osteoporosis regenerated scales to promote normal regeneration. Gene expression analysis revealed that 10-gingerol suppressed osteoclast markers in RAW264.7 cells [osteoclast-associated immunoglobulin-like receptor, dendrocyte-expressed seven transmembrane protein, and matrix metallopeptidase-9 (Mmp9)] and zebrafish scales [osteoclast-specific cathepsin K (CTSK), mmp2, and mmp9]. Interestingly, nuclear factor of activated T-cells cytoplasmic 1, a master transcription regulator of osteoclast differentiation upstream of the osteoclastic activators, was downregulated in zebrafish scales but showed no alteration in RAW264.7 cells. In addition, 10-gingerol inhibited CTSK activity under cell-free conditions. This is the first study, to our knowledge, that has found that 10-gingerol in GHE could suppress osteoclastic activity in both in vitro and in vivo conditions.
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spelling pubmed-79780332021-03-20 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales Zang, Liqing Kagotani, Kazuhiro Nakayama, Hiroko Bhagat, Jacky Fujimoto, Yuki Hayashi, Akihito Sono, Ryoji Katsuzaki, Hirotaka Nishimura, Norihiro Shimada, Yasuhito Front Cell Dev Biol Cell and Developmental Biology Osteoporosis is the most common aging-associated bone disease and is caused by hyperactivation of osteoclastic activity. We previously reported that the hexane extract of ginger rhizome [ginger hexane extract (GHE)] could suppress receptor activator of nuclear factor kappa-B ligand (RANKL)-induced osteoclastogenesis in RAW264.7 cells. However, the anti-osteoclastic components in GHE have not yet been identified. In this study, we separated GHE into several fractions using silica gel column chromatography and evaluated their effects on osteoclastogenesis using a RAW264.7 cell osteoclast differentiation assay (in vitro) and the zebrafish scale model of osteoporosis (in vivo). We identified that the fractions containing 10-gingerol suppressed osteoclastogenesis in RAW264.7 cells detected by tartrate-resistant acid phosphatase (TRAP) staining. In zebrafish, GHE and 10-gingerol suppressed osteoclastogenesis in prednisolone-induced osteoporosis regenerated scales to promote normal regeneration. Gene expression analysis revealed that 10-gingerol suppressed osteoclast markers in RAW264.7 cells [osteoclast-associated immunoglobulin-like receptor, dendrocyte-expressed seven transmembrane protein, and matrix metallopeptidase-9 (Mmp9)] and zebrafish scales [osteoclast-specific cathepsin K (CTSK), mmp2, and mmp9]. Interestingly, nuclear factor of activated T-cells cytoplasmic 1, a master transcription regulator of osteoclast differentiation upstream of the osteoclastic activators, was downregulated in zebrafish scales but showed no alteration in RAW264.7 cells. In addition, 10-gingerol inhibited CTSK activity under cell-free conditions. This is the first study, to our knowledge, that has found that 10-gingerol in GHE could suppress osteoclastic activity in both in vitro and in vivo conditions. Frontiers Media S.A. 2021-03-03 /pmc/articles/PMC7978033/ /pubmed/33748100 http://dx.doi.org/10.3389/fcell.2021.588093 Text en Copyright © 2021 Zang, Kagotani, Nakayama, Bhagat, Fujimoto, Hayashi, Sono, Katsuzaki, Nishimura and Shimada. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Zang, Liqing
Kagotani, Kazuhiro
Nakayama, Hiroko
Bhagat, Jacky
Fujimoto, Yuki
Hayashi, Akihito
Sono, Ryoji
Katsuzaki, Hirotaka
Nishimura, Norihiro
Shimada, Yasuhito
10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales
title 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales
title_full 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales
title_fullStr 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales
title_full_unstemmed 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales
title_short 10-Gingerol Suppresses Osteoclastogenesis in RAW264.7 Cells and Zebrafish Osteoporotic Scales
title_sort 10-gingerol suppresses osteoclastogenesis in raw264.7 cells and zebrafish osteoporotic scales
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978033/
https://www.ncbi.nlm.nih.gov/pubmed/33748100
http://dx.doi.org/10.3389/fcell.2021.588093
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