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Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation

Host-associated microbiomes can play important roles in the ecology and evolution of their insect hosts, but bacterial diversity in many insect groups remains poorly understood. Here we examine the relationship between host environment, host traits, and microbial diversity in three species in the gr...

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Autores principales: Silver, Anita, Perez, Sean, Gee, Melanie, Xu, Bethany, Garg, Shreeya, Will, Kipling, Gill, Aman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978345/
https://www.ncbi.nlm.nih.gov/pubmed/33739998
http://dx.doi.org/10.1371/journal.pone.0241529
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author Silver, Anita
Perez, Sean
Gee, Melanie
Xu, Bethany
Garg, Shreeya
Will, Kipling
Gill, Aman
author_facet Silver, Anita
Perez, Sean
Gee, Melanie
Xu, Bethany
Garg, Shreeya
Will, Kipling
Gill, Aman
author_sort Silver, Anita
collection PubMed
description Host-associated microbiomes can play important roles in the ecology and evolution of their insect hosts, but bacterial diversity in many insect groups remains poorly understood. Here we examine the relationship between host environment, host traits, and microbial diversity in three species in the ground beetle family (Coleoptera: Carabidae), a group of roughly 40,000 species that synthesize a wide diversity of defensive compounds. This study used 16S amplicon sequencing to profile three species that are phylogenetically distantly related, trophically distinct, and whose defensive chemical secretions differ: Anisodactylus similis LeConte, 1851, Pterostichus serripes (LeConte, 1875), and Brachinus elongatulus Chaudoir, 1876. Wild-caught beetles were compared to individuals maintained in the lab for two weeks on carnivorous, herbivorous, or starvation diets (n = 3 beetles for each species-diet combination). Metagenomic samples from two highly active tissue types—guts, and pygidial gland secretory cells (which produce defensive compounds)—were processed and sequenced separately from those of the remaining body. Bacterial composition and diversity of these ground beetles were largely resilient to controlled changes to host diet. Different tissues within the same beetle harbor unique microbial communities, and secretory cells in particular were remarkably similar across species. We also found that these three carabid species have patterns of microbial diversity similar to those previously found in carabid beetles. These results provide a baseline for future studies of the role of microbes in the diversification of carabids.
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spelling pubmed-79783452021-03-30 Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation Silver, Anita Perez, Sean Gee, Melanie Xu, Bethany Garg, Shreeya Will, Kipling Gill, Aman PLoS One Research Article Host-associated microbiomes can play important roles in the ecology and evolution of their insect hosts, but bacterial diversity in many insect groups remains poorly understood. Here we examine the relationship between host environment, host traits, and microbial diversity in three species in the ground beetle family (Coleoptera: Carabidae), a group of roughly 40,000 species that synthesize a wide diversity of defensive compounds. This study used 16S amplicon sequencing to profile three species that are phylogenetically distantly related, trophically distinct, and whose defensive chemical secretions differ: Anisodactylus similis LeConte, 1851, Pterostichus serripes (LeConte, 1875), and Brachinus elongatulus Chaudoir, 1876. Wild-caught beetles were compared to individuals maintained in the lab for two weeks on carnivorous, herbivorous, or starvation diets (n = 3 beetles for each species-diet combination). Metagenomic samples from two highly active tissue types—guts, and pygidial gland secretory cells (which produce defensive compounds)—were processed and sequenced separately from those of the remaining body. Bacterial composition and diversity of these ground beetles were largely resilient to controlled changes to host diet. Different tissues within the same beetle harbor unique microbial communities, and secretory cells in particular were remarkably similar across species. We also found that these three carabid species have patterns of microbial diversity similar to those previously found in carabid beetles. These results provide a baseline for future studies of the role of microbes in the diversification of carabids. Public Library of Science 2021-03-19 /pmc/articles/PMC7978345/ /pubmed/33739998 http://dx.doi.org/10.1371/journal.pone.0241529 Text en © 2021 Silver et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Silver, Anita
Perez, Sean
Gee, Melanie
Xu, Bethany
Garg, Shreeya
Will, Kipling
Gill, Aman
Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation
title Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation
title_full Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation
title_fullStr Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation
title_full_unstemmed Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation
title_short Persistence of the ground beetle (Coleoptera: Carabidae) microbiome to diet manipulation
title_sort persistence of the ground beetle (coleoptera: carabidae) microbiome to diet manipulation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7978345/
https://www.ncbi.nlm.nih.gov/pubmed/33739998
http://dx.doi.org/10.1371/journal.pone.0241529
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