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Anopheles ecology, genetics and malaria transmission in northern Cambodia
In the Greater Mekong Subregion, malaria cases have significantly decreased but little is known about the vectors or mechanisms responsible for residual malaria transmission. We analysed a total of 3920 Anopheles mosquitoes collected during the rainy and dry seasons from four ecological settings in...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979810/ https://www.ncbi.nlm.nih.gov/pubmed/33742030 http://dx.doi.org/10.1038/s41598-021-85628-1 |
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author | Vantaux, Amélie Riehle, Michelle M. Piv, Eakpor Farley, Elise J. Chy, Sophy Kim, Saorin Corbett, Anneli G. Fehrman, Rachel L. Pepey, Anais Eiglmeier, Karin Lek, Dysoley Siv, Sovannaroth Mueller, Ivo Vernick, Kenneth D. Witkowski, Benoit |
author_facet | Vantaux, Amélie Riehle, Michelle M. Piv, Eakpor Farley, Elise J. Chy, Sophy Kim, Saorin Corbett, Anneli G. Fehrman, Rachel L. Pepey, Anais Eiglmeier, Karin Lek, Dysoley Siv, Sovannaroth Mueller, Ivo Vernick, Kenneth D. Witkowski, Benoit |
author_sort | Vantaux, Amélie |
collection | PubMed |
description | In the Greater Mekong Subregion, malaria cases have significantly decreased but little is known about the vectors or mechanisms responsible for residual malaria transmission. We analysed a total of 3920 Anopheles mosquitoes collected during the rainy and dry seasons from four ecological settings in Cambodia (villages, forested areas near villages, rubber tree plantations and forest sites). Using odor-baited traps, 81% of the total samples across all sites were collected in cow baited traps, although 67% of the samples attracted by human baited traps were collected in forest sites. Overall, 20% of collected Anopheles were active during the day, with increased day biting during the dry season. 3131 samples were identified morphologically as 14 different species, and a subset was also identified by DNA amplicon sequencing allowing determination of 29 Anopheles species. The investigation of well characterized insecticide mutations (ace-1, kdr, and rdl genes) indicated that individuals carried mutations associated with response to all the different classes of insecticides. There also appeared to be a non-random association between mosquito species and insecticide resistance with Anopheles peditaeniatus exhibiting nearly fixed mutations. Molecular screening for Plasmodium sp. presence indicated that 3.6% of collected Anopheles were positive, most for P. vivax followed by P. falciparum. These results highlight some of the key mechanisms driving residual human malaria transmission in Cambodia, and illustrate the importance of diverse collection methods, sites and seasons to avoid bias and better characterize Anopheles mosquito ecology in Southeast Asia. |
format | Online Article Text |
id | pubmed-7979810 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-79798102021-03-25 Anopheles ecology, genetics and malaria transmission in northern Cambodia Vantaux, Amélie Riehle, Michelle M. Piv, Eakpor Farley, Elise J. Chy, Sophy Kim, Saorin Corbett, Anneli G. Fehrman, Rachel L. Pepey, Anais Eiglmeier, Karin Lek, Dysoley Siv, Sovannaroth Mueller, Ivo Vernick, Kenneth D. Witkowski, Benoit Sci Rep Article In the Greater Mekong Subregion, malaria cases have significantly decreased but little is known about the vectors or mechanisms responsible for residual malaria transmission. We analysed a total of 3920 Anopheles mosquitoes collected during the rainy and dry seasons from four ecological settings in Cambodia (villages, forested areas near villages, rubber tree plantations and forest sites). Using odor-baited traps, 81% of the total samples across all sites were collected in cow baited traps, although 67% of the samples attracted by human baited traps were collected in forest sites. Overall, 20% of collected Anopheles were active during the day, with increased day biting during the dry season. 3131 samples were identified morphologically as 14 different species, and a subset was also identified by DNA amplicon sequencing allowing determination of 29 Anopheles species. The investigation of well characterized insecticide mutations (ace-1, kdr, and rdl genes) indicated that individuals carried mutations associated with response to all the different classes of insecticides. There also appeared to be a non-random association between mosquito species and insecticide resistance with Anopheles peditaeniatus exhibiting nearly fixed mutations. Molecular screening for Plasmodium sp. presence indicated that 3.6% of collected Anopheles were positive, most for P. vivax followed by P. falciparum. These results highlight some of the key mechanisms driving residual human malaria transmission in Cambodia, and illustrate the importance of diverse collection methods, sites and seasons to avoid bias and better characterize Anopheles mosquito ecology in Southeast Asia. Nature Publishing Group UK 2021-03-19 /pmc/articles/PMC7979810/ /pubmed/33742030 http://dx.doi.org/10.1038/s41598-021-85628-1 Text en © The Author(s) 2021, corrected publication 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Vantaux, Amélie Riehle, Michelle M. Piv, Eakpor Farley, Elise J. Chy, Sophy Kim, Saorin Corbett, Anneli G. Fehrman, Rachel L. Pepey, Anais Eiglmeier, Karin Lek, Dysoley Siv, Sovannaroth Mueller, Ivo Vernick, Kenneth D. Witkowski, Benoit Anopheles ecology, genetics and malaria transmission in northern Cambodia |
title | Anopheles ecology, genetics and malaria transmission in northern Cambodia |
title_full | Anopheles ecology, genetics and malaria transmission in northern Cambodia |
title_fullStr | Anopheles ecology, genetics and malaria transmission in northern Cambodia |
title_full_unstemmed | Anopheles ecology, genetics and malaria transmission in northern Cambodia |
title_short | Anopheles ecology, genetics and malaria transmission in northern Cambodia |
title_sort | anopheles ecology, genetics and malaria transmission in northern cambodia |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979810/ https://www.ncbi.nlm.nih.gov/pubmed/33742030 http://dx.doi.org/10.1038/s41598-021-85628-1 |
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