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Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions

Mammalian three-dimensional (3D) enteroids mirror in vivo intestinal organisation and are powerful tools to investigate intestinal cell biology and host–pathogen interactions. We have developed complex multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts. These avian...

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Detalles Bibliográficos
Autores principales: Nash, Tessa J., Morris, Katrina M., Mabbott, Neil A., Vervelde, Lonneke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979936/
https://www.ncbi.nlm.nih.gov/pubmed/33742093
http://dx.doi.org/10.1038/s42003-021-01901-z
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author Nash, Tessa J.
Morris, Katrina M.
Mabbott, Neil A.
Vervelde, Lonneke
author_facet Nash, Tessa J.
Morris, Katrina M.
Mabbott, Neil A.
Vervelde, Lonneke
author_sort Nash, Tessa J.
collection PubMed
description Mammalian three-dimensional (3D) enteroids mirror in vivo intestinal organisation and are powerful tools to investigate intestinal cell biology and host–pathogen interactions. We have developed complex multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts. These avian enteroids develop optimally in suspension without the structural support required to produce mammalian enteroids, resulting in an inside-out enteroid conformation with media-facing apical brush borders. Histological and transcriptional analyses show these enteroids comprise of differentiated intestinal epithelial cells bound by cell-cell junctions, and notably, include intraepithelial leukocytes and an inner core of lamina propria leukocytes. The advantageous polarisation of these enteroids has enabled infection of the epithelial apical surface with Salmonella Typhimurium, influenza A virus and Eimeria tenella without the need for micro-injection. We have created a comprehensive model of the chicken intestine which has the potential to explore epithelial and leukocyte interactions and responses in host–pathogen, food science and pharmaceutical research.
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spelling pubmed-79799362021-04-12 Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions Nash, Tessa J. Morris, Katrina M. Mabbott, Neil A. Vervelde, Lonneke Commun Biol Article Mammalian three-dimensional (3D) enteroids mirror in vivo intestinal organisation and are powerful tools to investigate intestinal cell biology and host–pathogen interactions. We have developed complex multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts. These avian enteroids develop optimally in suspension without the structural support required to produce mammalian enteroids, resulting in an inside-out enteroid conformation with media-facing apical brush borders. Histological and transcriptional analyses show these enteroids comprise of differentiated intestinal epithelial cells bound by cell-cell junctions, and notably, include intraepithelial leukocytes and an inner core of lamina propria leukocytes. The advantageous polarisation of these enteroids has enabled infection of the epithelial apical surface with Salmonella Typhimurium, influenza A virus and Eimeria tenella without the need for micro-injection. We have created a comprehensive model of the chicken intestine which has the potential to explore epithelial and leukocyte interactions and responses in host–pathogen, food science and pharmaceutical research. Nature Publishing Group UK 2021-03-19 /pmc/articles/PMC7979936/ /pubmed/33742093 http://dx.doi.org/10.1038/s42003-021-01901-z Text en © The Author(s) 2021 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Nash, Tessa J.
Morris, Katrina M.
Mabbott, Neil A.
Vervelde, Lonneke
Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
title Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
title_full Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
title_fullStr Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
title_full_unstemmed Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
title_short Inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
title_sort inside-out chicken enteroids with leukocyte component as a model to study host–pathogen interactions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7979936/
https://www.ncbi.nlm.nih.gov/pubmed/33742093
http://dx.doi.org/10.1038/s42003-021-01901-z
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