Cargando…

RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice

BACKGROUND: Carotenoid biosynthesis is essential for the generation of photosynthetic pigments, phytohormone production, and flower color development. The light harvesting like 3 (LIL3) protein, which belongs to the light-harvesting complex protein family in photosystems, interacts with geranylgeran...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Feng, Debatosh, Das, Song, Tao, Zhang, Jian-hua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981378/
https://www.ncbi.nlm.nih.gov/pubmed/33745012
http://dx.doi.org/10.1186/s12284-021-00474-z
_version_ 1783667537173544960
author Yang, Feng
Debatosh, Das
Song, Tao
Zhang, Jian-hua
author_facet Yang, Feng
Debatosh, Das
Song, Tao
Zhang, Jian-hua
author_sort Yang, Feng
collection PubMed
description BACKGROUND: Carotenoid biosynthesis is essential for the generation of photosynthetic pigments, phytohormone production, and flower color development. The light harvesting like 3 (LIL3) protein, which belongs to the light-harvesting complex protein family in photosystems, interacts with geranylgeranyl reductase (GGR) and protochlorophyllide oxidoreductase (POR) both of which are known to regulate terpenoid and chlorophyll biosynthesis, respectively, in both rice and Arabidopsis. RESULTS: In our study, a CRISPR-Cas9 generated 4-bp deletion mutant oslil3 showed aberrant chloroplast development, growth defects, low fertility rates and reduced pigment contents. A comparative transcriptomic analysis of oslil3 suggested that differentially expressed genes (DEGs) involved in photosynthesis, cell wall modification, primary and secondary metabolism are differentially regulated in the mutant. Protein-protein interaction assays indicated that LIL3 interacts with phytoene synthase (PSY) and in addition the gene expression of PSY genes are regulated by LIL3. Subcellular localization of LIL3 and PSY suggested that both are thylakoid membrane anchored proteins in the chloroplast. We suggest that LIL3 directly interacts with PSY to regulate carotenoid biosynthesis. CONCLUSION: This study reveals a new role of LIL3 in regulating pigment biosynthesis through interaction with the rate limiting enzyme PSY in carotenoid biosynthesis in rice presenting it as a putative target for genetic manipulation of pigment biosynthesis pathways in crop plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-021-00474-z.
format Online
Article
Text
id pubmed-7981378
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-79813782021-04-12 RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice Yang, Feng Debatosh, Das Song, Tao Zhang, Jian-hua Rice (N Y) Original Article BACKGROUND: Carotenoid biosynthesis is essential for the generation of photosynthetic pigments, phytohormone production, and flower color development. The light harvesting like 3 (LIL3) protein, which belongs to the light-harvesting complex protein family in photosystems, interacts with geranylgeranyl reductase (GGR) and protochlorophyllide oxidoreductase (POR) both of which are known to regulate terpenoid and chlorophyll biosynthesis, respectively, in both rice and Arabidopsis. RESULTS: In our study, a CRISPR-Cas9 generated 4-bp deletion mutant oslil3 showed aberrant chloroplast development, growth defects, low fertility rates and reduced pigment contents. A comparative transcriptomic analysis of oslil3 suggested that differentially expressed genes (DEGs) involved in photosynthesis, cell wall modification, primary and secondary metabolism are differentially regulated in the mutant. Protein-protein interaction assays indicated that LIL3 interacts with phytoene synthase (PSY) and in addition the gene expression of PSY genes are regulated by LIL3. Subcellular localization of LIL3 and PSY suggested that both are thylakoid membrane anchored proteins in the chloroplast. We suggest that LIL3 directly interacts with PSY to regulate carotenoid biosynthesis. CONCLUSION: This study reveals a new role of LIL3 in regulating pigment biosynthesis through interaction with the rate limiting enzyme PSY in carotenoid biosynthesis in rice presenting it as a putative target for genetic manipulation of pigment biosynthesis pathways in crop plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12284-021-00474-z. Springer US 2021-03-20 /pmc/articles/PMC7981378/ /pubmed/33745012 http://dx.doi.org/10.1186/s12284-021-00474-z Text en © The Author(s). 2021 https://creativecommons.org/licenses/by/4.0/ Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Yang, Feng
Debatosh, Das
Song, Tao
Zhang, Jian-hua
RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice
title RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice
title_full RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice
title_fullStr RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice
title_full_unstemmed RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice
title_short RETRACTED ARTICLE: Light Harvesting-like Protein 3 Interacts with Phytoene Synthase and Is Necessary for Carotenoid and Chlorophyll Biosynthesis in Rice
title_sort retracted article: light harvesting-like protein 3 interacts with phytoene synthase and is necessary for carotenoid and chlorophyll biosynthesis in rice
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981378/
https://www.ncbi.nlm.nih.gov/pubmed/33745012
http://dx.doi.org/10.1186/s12284-021-00474-z
work_keys_str_mv AT yangfeng retractedarticlelightharvestinglikeprotein3interactswithphytoenesynthaseandisnecessaryforcarotenoidandchlorophyllbiosynthesisinrice
AT debatoshdas retractedarticlelightharvestinglikeprotein3interactswithphytoenesynthaseandisnecessaryforcarotenoidandchlorophyllbiosynthesisinrice
AT songtao retractedarticlelightharvestinglikeprotein3interactswithphytoenesynthaseandisnecessaryforcarotenoidandchlorophyllbiosynthesisinrice
AT zhangjianhua retractedarticlelightharvestinglikeprotein3interactswithphytoenesynthaseandisnecessaryforcarotenoidandchlorophyllbiosynthesisinrice