Standard protocol for the PIGRET assay, a high-throughput reticulocyte Pig-a assay with an immunomagnetic separation, used in the interlaboratory trial organized by the Mammalian Mutagenicity Study Group of the Japanese Environmental Mutagen and Genome Society

The PIGRET assay is one of the Pig-a assays targeting reticulocytes (RETs), an in vivo genotoxicity evaluation method using flow cytometry with endogenous reporter glycosylphosphatidylinositol anchor protein. The PIGRET assay with RETs selectively enriched with anti-CD71 antibodies has several desir...

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Detalles Bibliográficos
Autores principales: Chikura, Satsuki, Kimoto, Takafumi, Itoh, Satoru, Sanada, Hisakazu, Muto, Shigeharu, Horibata, Katsuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7981892/
https://www.ncbi.nlm.nih.gov/pubmed/33743813
http://dx.doi.org/10.1186/s41021-021-00181-7

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