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Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice

The main aim of this study was to examine if a female mouse body in preimplantation pregnancy can distinguish between embryos of normal and impaired biological quality in the local and peripheral compartments. Normal (control group) and TNFα (tumor necrosis factor-α)-treated embryos (experimental gr...

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Autores principales: Buska-Mach, Katarzyna, Kedzierska, Anna Ewa, Lepczynski, Adam, Herosimczyk, Agnieszka, Ozgo, Małgorzata, Karpinski, Pawel, Gomulkiewicz, Agnieszka, Lorek, Daria, Slawek, Anna, Dziegiel, Piotr, Chelmonska-Soyta, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982469/
https://www.ncbi.nlm.nih.gov/pubmed/33763465
http://dx.doi.org/10.3389/fvets.2021.641553
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author Buska-Mach, Katarzyna
Kedzierska, Anna Ewa
Lepczynski, Adam
Herosimczyk, Agnieszka
Ozgo, Małgorzata
Karpinski, Pawel
Gomulkiewicz, Agnieszka
Lorek, Daria
Slawek, Anna
Dziegiel, Piotr
Chelmonska-Soyta, Anna
author_facet Buska-Mach, Katarzyna
Kedzierska, Anna Ewa
Lepczynski, Adam
Herosimczyk, Agnieszka
Ozgo, Małgorzata
Karpinski, Pawel
Gomulkiewicz, Agnieszka
Lorek, Daria
Slawek, Anna
Dziegiel, Piotr
Chelmonska-Soyta, Anna
author_sort Buska-Mach, Katarzyna
collection PubMed
description The main aim of this study was to examine if a female mouse body in preimplantation pregnancy can distinguish between embryos of normal and impaired biological quality in the local and peripheral compartments. Normal (control group) and TNFα (tumor necrosis factor-α)-treated embryos (experimental group) at the morula stage were non-surgically transferred into the uteri of CD-1 strain [Crl:CD1(Icr)] female murine recipients. Twenty-four hours after the embryo transfer, females were euthanised, and uteri and spleens were dissected. In uterine tissues (local compartment), we assessed the expression of 84 genes comprising nine signal transduction pathways, using a modified RT(2) Profiler PCR Array. In the spleen (peripheral compartment), we determined the proteome of splenic CD4(+) lymphocytes using 2D protein electrophoresis with subsequent protein identification by mass spectrometry. Sample clustering and differential gene expression analyses within individual signal transduction pathways revealed differential expression of genes in the uteri of females after transplantation of normal vs. TNFα-treated embryos. The most affected signal transduction cascade was the NFKB (Nuclear factor NF-kappa-B) pathway, where 87.5% of the examined genes were significantly differentially expressed. Proteomic analysis of splenic CD4(+) T lymphocytes revealed significant differential expression of 8 out of 132 protein spots. Identified proteins were classified as proteins influenced by cell stress, proteins engaged in the regulation of cytoskeleton stabilization and cell motility, and proteins having immunomodulatory function. These results support the hypothesis that even before embryo implantation, the body of pregnant female mice can sense the biological quality of an embryo both at the local and peripheral level.
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spelling pubmed-79824692021-03-23 Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice Buska-Mach, Katarzyna Kedzierska, Anna Ewa Lepczynski, Adam Herosimczyk, Agnieszka Ozgo, Małgorzata Karpinski, Pawel Gomulkiewicz, Agnieszka Lorek, Daria Slawek, Anna Dziegiel, Piotr Chelmonska-Soyta, Anna Front Vet Sci Veterinary Science The main aim of this study was to examine if a female mouse body in preimplantation pregnancy can distinguish between embryos of normal and impaired biological quality in the local and peripheral compartments. Normal (control group) and TNFα (tumor necrosis factor-α)-treated embryos (experimental group) at the morula stage were non-surgically transferred into the uteri of CD-1 strain [Crl:CD1(Icr)] female murine recipients. Twenty-four hours after the embryo transfer, females were euthanised, and uteri and spleens were dissected. In uterine tissues (local compartment), we assessed the expression of 84 genes comprising nine signal transduction pathways, using a modified RT(2) Profiler PCR Array. In the spleen (peripheral compartment), we determined the proteome of splenic CD4(+) lymphocytes using 2D protein electrophoresis with subsequent protein identification by mass spectrometry. Sample clustering and differential gene expression analyses within individual signal transduction pathways revealed differential expression of genes in the uteri of females after transplantation of normal vs. TNFα-treated embryos. The most affected signal transduction cascade was the NFKB (Nuclear factor NF-kappa-B) pathway, where 87.5% of the examined genes were significantly differentially expressed. Proteomic analysis of splenic CD4(+) T lymphocytes revealed significant differential expression of 8 out of 132 protein spots. Identified proteins were classified as proteins influenced by cell stress, proteins engaged in the regulation of cytoskeleton stabilization and cell motility, and proteins having immunomodulatory function. These results support the hypothesis that even before embryo implantation, the body of pregnant female mice can sense the biological quality of an embryo both at the local and peripheral level. Frontiers Media S.A. 2021-03-08 /pmc/articles/PMC7982469/ /pubmed/33763465 http://dx.doi.org/10.3389/fvets.2021.641553 Text en Copyright © 2021 Buska-Mach, Kedzierska, Lepczynski, Herosimczyk, Ozgo, Karpinski, Gomulkiewicz, Lorek, Slawek, Dziegiel and Chelmonska-Soyta. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Buska-Mach, Katarzyna
Kedzierska, Anna Ewa
Lepczynski, Adam
Herosimczyk, Agnieszka
Ozgo, Małgorzata
Karpinski, Pawel
Gomulkiewicz, Agnieszka
Lorek, Daria
Slawek, Anna
Dziegiel, Piotr
Chelmonska-Soyta, Anna
Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice
title Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice
title_full Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice
title_fullStr Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice
title_full_unstemmed Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice
title_short Differential Signals From TNFα-Treated and Untreated Embryos in Uterine Tissues and Splenic CD4(+) T Lymphocytes During Preimplantation Pregnancy in Mice
title_sort differential signals from tnfα-treated and untreated embryos in uterine tissues and splenic cd4(+) t lymphocytes during preimplantation pregnancy in mice
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982469/
https://www.ncbi.nlm.nih.gov/pubmed/33763465
http://dx.doi.org/10.3389/fvets.2021.641553
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