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Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative

Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in two adult Thoroughbred stallions as part of the Functional Annotation of the Animal Genome (FAANG) initiative. Both stallions underwent thorough physical, lameness,...

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Autores principales: Donnelly, Callum G., Bellone, Rebecca R., Hales, Erin N., Nguyen, Annee, Katzman, Scott A., Dujovne, Ghislaine A., Knickelbein, Kelly E., Avila, Felipe, Kalbfleisch, Ted S., Giulotto, Elena, Kingsley, Nicole B., Tanaka, Jocelyn, Esdaile, Elizabeth, Peng, Sichong, Dahlgren, Anna, Fuller, Anna, Mienaltowski, Michael J., Raudsepp, Terje, Affolter, Verena K., Petersen, Jessica L., Finno, Carrie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982670/
https://www.ncbi.nlm.nih.gov/pubmed/33763124
http://dx.doi.org/10.3389/fgene.2021.650305
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author Donnelly, Callum G.
Bellone, Rebecca R.
Hales, Erin N.
Nguyen, Annee
Katzman, Scott A.
Dujovne, Ghislaine A.
Knickelbein, Kelly E.
Avila, Felipe
Kalbfleisch, Ted S.
Giulotto, Elena
Kingsley, Nicole B.
Tanaka, Jocelyn
Esdaile, Elizabeth
Peng, Sichong
Dahlgren, Anna
Fuller, Anna
Mienaltowski, Michael J.
Raudsepp, Terje
Affolter, Verena K.
Petersen, Jessica L.
Finno, Carrie J.
author_facet Donnelly, Callum G.
Bellone, Rebecca R.
Hales, Erin N.
Nguyen, Annee
Katzman, Scott A.
Dujovne, Ghislaine A.
Knickelbein, Kelly E.
Avila, Felipe
Kalbfleisch, Ted S.
Giulotto, Elena
Kingsley, Nicole B.
Tanaka, Jocelyn
Esdaile, Elizabeth
Peng, Sichong
Dahlgren, Anna
Fuller, Anna
Mienaltowski, Michael J.
Raudsepp, Terje
Affolter, Verena K.
Petersen, Jessica L.
Finno, Carrie J.
author_sort Donnelly, Callum G.
collection PubMed
description Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in two adult Thoroughbred stallions as part of the Functional Annotation of the Animal Genome (FAANG) initiative. Both stallions underwent thorough physical, lameness, neurologic, and ophthalmic (including electroretinography) examinations prior to humane euthanasia. Epididymal sperm was recovered from both stallions immediately postmortem and cryopreserved. Aseptically collected full thickness skin biopsies were used to isolate, culture and cryopreserve dermal fibroblasts. Serum, plasma, cerebrospinal fluid, urine, and gastrointestinal content from various locations were collected and cryopreserved. Under guidance of a board-certified veterinary anatomic pathologist, 102 representative tissue samples were collected from both horses. Whole tissue samples were flash-frozen and prioritized tissues had nuclei isolated and cryopreserved. Spatially contemporaneous samples of each tissue were submitted for histologic examination. Antemortem and gross pathologic examination revealed mild abnormalities in both stallions. One stallion (ECA_UCD_AH3) had unilateral thoracic limb lameness and bilateral chorioretinal scars. The second stallion (ECA_UCD_AH4) had subtle symmetrical pelvic limb ataxia, symmetrical prostatomegally, and moderate gastrointestinal nematodiasis. DNA from each was whole-genome sequenced and genotyped using the GGP Equine 70K SNP array. The genomic resources and banked biological samples from these animals augments the existing resource available to the equine genomics community. Importantly we may now improve the resolution of tissue-specific gene regulation as affected by sex, as well as add sex-specific tissues and gametes.
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spelling pubmed-79826702021-03-23 Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative Donnelly, Callum G. Bellone, Rebecca R. Hales, Erin N. Nguyen, Annee Katzman, Scott A. Dujovne, Ghislaine A. Knickelbein, Kelly E. Avila, Felipe Kalbfleisch, Ted S. Giulotto, Elena Kingsley, Nicole B. Tanaka, Jocelyn Esdaile, Elizabeth Peng, Sichong Dahlgren, Anna Fuller, Anna Mienaltowski, Michael J. Raudsepp, Terje Affolter, Verena K. Petersen, Jessica L. Finno, Carrie J. Front Genet Genetics Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in two adult Thoroughbred stallions as part of the Functional Annotation of the Animal Genome (FAANG) initiative. Both stallions underwent thorough physical, lameness, neurologic, and ophthalmic (including electroretinography) examinations prior to humane euthanasia. Epididymal sperm was recovered from both stallions immediately postmortem and cryopreserved. Aseptically collected full thickness skin biopsies were used to isolate, culture and cryopreserve dermal fibroblasts. Serum, plasma, cerebrospinal fluid, urine, and gastrointestinal content from various locations were collected and cryopreserved. Under guidance of a board-certified veterinary anatomic pathologist, 102 representative tissue samples were collected from both horses. Whole tissue samples were flash-frozen and prioritized tissues had nuclei isolated and cryopreserved. Spatially contemporaneous samples of each tissue were submitted for histologic examination. Antemortem and gross pathologic examination revealed mild abnormalities in both stallions. One stallion (ECA_UCD_AH3) had unilateral thoracic limb lameness and bilateral chorioretinal scars. The second stallion (ECA_UCD_AH4) had subtle symmetrical pelvic limb ataxia, symmetrical prostatomegally, and moderate gastrointestinal nematodiasis. DNA from each was whole-genome sequenced and genotyped using the GGP Equine 70K SNP array. The genomic resources and banked biological samples from these animals augments the existing resource available to the equine genomics community. Importantly we may now improve the resolution of tissue-specific gene regulation as affected by sex, as well as add sex-specific tissues and gametes. Frontiers Media S.A. 2021-03-08 /pmc/articles/PMC7982670/ /pubmed/33763124 http://dx.doi.org/10.3389/fgene.2021.650305 Text en Copyright © 2021 Donnelly, Bellone, Hales, Nguyen, Katzman, Dujovne, Knickelbein, Avila, Kalbfleisch, Giulotto, Kingsley, Tanaka, Esdaile, Peng, Dahlgren, Fuller, Mienaltowski, Raudsepp, Affolter, Petersen and Finno. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Donnelly, Callum G.
Bellone, Rebecca R.
Hales, Erin N.
Nguyen, Annee
Katzman, Scott A.
Dujovne, Ghislaine A.
Knickelbein, Kelly E.
Avila, Felipe
Kalbfleisch, Ted S.
Giulotto, Elena
Kingsley, Nicole B.
Tanaka, Jocelyn
Esdaile, Elizabeth
Peng, Sichong
Dahlgren, Anna
Fuller, Anna
Mienaltowski, Michael J.
Raudsepp, Terje
Affolter, Verena K.
Petersen, Jessica L.
Finno, Carrie J.
Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative
title Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative
title_full Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative
title_fullStr Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative
title_full_unstemmed Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative
title_short Generation of a Biobank From Two Adult Thoroughbred Stallions for the Functional Annotation of Animal Genomes Initiative
title_sort generation of a biobank from two adult thoroughbred stallions for the functional annotation of animal genomes initiative
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982670/
https://www.ncbi.nlm.nih.gov/pubmed/33763124
http://dx.doi.org/10.3389/fgene.2021.650305
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