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High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing

Diabetes-related skin problems represent the most common long-term complications in diabetes mellitus patients. These complications, which include diabetic dermopathy, diabetic blisters, necrobiosis lipoidica diabeticorum, and eruptive xanthomatosis, may dramatically impair patients’ quality of life...

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Autores principales: Zhang, Shan, Ke, Zunxiang, Yang, Chao, Zhou, Peng, Jiang, Huanzong, Chen, Lei, Li, Yiqing, Li, Qin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982678/
https://www.ncbi.nlm.nih.gov/pubmed/33763026
http://dx.doi.org/10.3389/fendo.2021.603645
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author Zhang, Shan
Ke, Zunxiang
Yang, Chao
Zhou, Peng
Jiang, Huanzong
Chen, Lei
Li, Yiqing
Li, Qin
author_facet Zhang, Shan
Ke, Zunxiang
Yang, Chao
Zhou, Peng
Jiang, Huanzong
Chen, Lei
Li, Yiqing
Li, Qin
author_sort Zhang, Shan
collection PubMed
description Diabetes-related skin problems represent the most common long-term complications in diabetes mellitus patients. These complications, which include diabetic dermopathy, diabetic blisters, necrobiosis lipoidica diabeticorum, and eruptive xanthomatosis, may dramatically impair patients’ quality of life and cause long-lasting disability. However, the cellular and molecular mechanisms linking diabetes-related hyperglycemia and skin complications are still incompletely understood. To assess the role of the various skin-cell types in hyperglycemia-induced skin disorders, we performed RNA sequencing-based transcriptome analysis, measuring gene expression patterns in biological replicates in normal- and high glucose-stimulated skin cells. Three primary human skin-cell types were examined, i.e., epidermal keratinocytes, dermal fibroblasts, and dermal microvascular endothelial cells. For each separate cell type, we identified gene expression. Comparing gene abundances and expression levels revealed that transcription profiles exhibit distinct patterns in the three skin-cell types exposed to normal (i.e., physiological) glucose treatment and high (i.e., supraphysiological) glucose treatment. The obtained data indicate that high glucose induced differential gene expression and distinct activity patterns in signaling pathways in each skin-cell type. We are adding these data to the public database in the hope that they will facilitate future studies to develop novel targeted interventions for diabetic skin complications.
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spelling pubmed-79826782021-03-23 High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing Zhang, Shan Ke, Zunxiang Yang, Chao Zhou, Peng Jiang, Huanzong Chen, Lei Li, Yiqing Li, Qin Front Endocrinol (Lausanne) Endocrinology Diabetes-related skin problems represent the most common long-term complications in diabetes mellitus patients. These complications, which include diabetic dermopathy, diabetic blisters, necrobiosis lipoidica diabeticorum, and eruptive xanthomatosis, may dramatically impair patients’ quality of life and cause long-lasting disability. However, the cellular and molecular mechanisms linking diabetes-related hyperglycemia and skin complications are still incompletely understood. To assess the role of the various skin-cell types in hyperglycemia-induced skin disorders, we performed RNA sequencing-based transcriptome analysis, measuring gene expression patterns in biological replicates in normal- and high glucose-stimulated skin cells. Three primary human skin-cell types were examined, i.e., epidermal keratinocytes, dermal fibroblasts, and dermal microvascular endothelial cells. For each separate cell type, we identified gene expression. Comparing gene abundances and expression levels revealed that transcription profiles exhibit distinct patterns in the three skin-cell types exposed to normal (i.e., physiological) glucose treatment and high (i.e., supraphysiological) glucose treatment. The obtained data indicate that high glucose induced differential gene expression and distinct activity patterns in signaling pathways in each skin-cell type. We are adding these data to the public database in the hope that they will facilitate future studies to develop novel targeted interventions for diabetic skin complications. Frontiers Media S.A. 2021-03-08 /pmc/articles/PMC7982678/ /pubmed/33763026 http://dx.doi.org/10.3389/fendo.2021.603645 Text en Copyright © 2021 Zhang, Ke, Yang, Zhou, Jiang, Chen, Li and Li http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Zhang, Shan
Ke, Zunxiang
Yang, Chao
Zhou, Peng
Jiang, Huanzong
Chen, Lei
Li, Yiqing
Li, Qin
High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing
title High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing
title_full High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing
title_fullStr High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing
title_full_unstemmed High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing
title_short High Glucose Causes Distinct Expression Patterns of Primary Human Skin Cells by RNA Sequencing
title_sort high glucose causes distinct expression patterns of primary human skin cells by rna sequencing
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7982678/
https://www.ncbi.nlm.nih.gov/pubmed/33763026
http://dx.doi.org/10.3389/fendo.2021.603645
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