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Injection of Evans blue dye to fluorescently label and image intact vasculature
Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodol...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Future Science Ltd
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983036/ https://www.ncbi.nlm.nih.gov/pubmed/33337254 http://dx.doi.org/10.2144/btn-2020-0152 |
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author | Honeycutt, Samuel E O'Brien, Lori L |
author_facet | Honeycutt, Samuel E O'Brien, Lori L |
author_sort | Honeycutt, Samuel E |
collection | PubMed |
description | Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks in vivo by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods. |
format | Online Article Text |
id | pubmed-7983036 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Future Science Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-79830362021-03-25 Injection of Evans blue dye to fluorescently label and image intact vasculature Honeycutt, Samuel E O'Brien, Lori L Biotechniques Benchmark Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks in vivo by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods. Future Science Ltd 2020-12-18 2021-03 /pmc/articles/PMC7983036/ /pubmed/33337254 http://dx.doi.org/10.2144/btn-2020-0152 Text en © 2020 Lori L. O'Brien This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Benchmark Honeycutt, Samuel E O'Brien, Lori L Injection of Evans blue dye to fluorescently label and image intact vasculature |
title | Injection of Evans blue dye to fluorescently label and image intact vasculature |
title_full | Injection of Evans blue dye to fluorescently label and image intact vasculature |
title_fullStr | Injection of Evans blue dye to fluorescently label and image intact vasculature |
title_full_unstemmed | Injection of Evans blue dye to fluorescently label and image intact vasculature |
title_short | Injection of Evans blue dye to fluorescently label and image intact vasculature |
title_sort | injection of evans blue dye to fluorescently label and image intact vasculature |
topic | Benchmark |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983036/ https://www.ncbi.nlm.nih.gov/pubmed/33337254 http://dx.doi.org/10.2144/btn-2020-0152 |
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