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Injection of Evans blue dye to fluorescently label and image intact vasculature

Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodol...

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Detalles Bibliográficos
Autores principales: Honeycutt, Samuel E, O'Brien, Lori L
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Future Science Ltd 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983036/
https://www.ncbi.nlm.nih.gov/pubmed/33337254
http://dx.doi.org/10.2144/btn-2020-0152
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author Honeycutt, Samuel E
O'Brien, Lori L
author_facet Honeycutt, Samuel E
O'Brien, Lori L
author_sort Honeycutt, Samuel E
collection PubMed
description Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks in vivo by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods.
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spelling pubmed-79830362021-03-25 Injection of Evans blue dye to fluorescently label and image intact vasculature Honeycutt, Samuel E O'Brien, Lori L Biotechniques Benchmark Blood vessels perform critical functions in both health and disease. Understanding how vessels form, pattern and respond to damage is essential. However, labeling and imaging the vasculature to ascertain these properties can be difficult and time-consuming. Here, the authors present a novel methodology for rapidly and efficiently labeling whole vascular networks in vivo by exploiting the fluorescent properties of Evans blue. By combining the labeling with fluorescence microscopy, this method enables visualization of whole tissue vasculature for a fraction of the time and cost compared with traditional methods. Future Science Ltd 2020-12-18 2021-03 /pmc/articles/PMC7983036/ /pubmed/33337254 http://dx.doi.org/10.2144/btn-2020-0152 Text en © 2020 Lori L. O'Brien This work is licensed under the Attribution-NonCommercial-NoDerivatives 4.0 Unported License (http://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Benchmark
Honeycutt, Samuel E
O'Brien, Lori L
Injection of Evans blue dye to fluorescently label and image intact vasculature
title Injection of Evans blue dye to fluorescently label and image intact vasculature
title_full Injection of Evans blue dye to fluorescently label and image intact vasculature
title_fullStr Injection of Evans blue dye to fluorescently label and image intact vasculature
title_full_unstemmed Injection of Evans blue dye to fluorescently label and image intact vasculature
title_short Injection of Evans blue dye to fluorescently label and image intact vasculature
title_sort injection of evans blue dye to fluorescently label and image intact vasculature
topic Benchmark
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983036/
https://www.ncbi.nlm.nih.gov/pubmed/33337254
http://dx.doi.org/10.2144/btn-2020-0152
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