Adding further evidence for clinically significant anti-Le(b) antibody in a voluntary blood donor

Herein, we report a case of naturally occurring anti-Le(b) alloantibody identified in the plasma of a first time voluntary blood donor. The immunohematology workup was done on the pilot sample tubes collected during blood donation by the conventional tube technique and using ID-Micro Column System Glass Beads card (anti-IgG, C(3)d; Ortho-Clinical Diagnostics, Raritan, New Jersey, USA). Blood group of the donor was confirmed to be B RhD positive, and the alloantibody in his plasma was identified as anti-Le(b), having clinically significant characteristics. Since in this particular case, anti-Le(b) was IgM and IgG in nature, it was clinically significant and can lead to hemolytic transfusion reaction, especially if such fresh frozen plasma unit is transfused to Le(b) negative patients.