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PM2.5 inducing myocardial fibrosis mediated by Ang II/ERK1/2/TGF-β(1) signaling pathway in mice model
OBJECTS: To discuss the influence of PM2.5 on myocardial fibrosis and related mechanism. METHODS: PM2.5 particles were prepared into different concentrations of solution to drip into the mice’s trachea twice each week. The mice were divided into five groups, Blank control group (C group), NS control...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983242/ https://www.ncbi.nlm.nih.gov/pubmed/33726569 http://dx.doi.org/10.1177/14703203211003786 |
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author | Zang, Xiwen Zhao, Jun Lu, Chengzhi |
author_facet | Zang, Xiwen Zhao, Jun Lu, Chengzhi |
author_sort | Zang, Xiwen |
collection | PubMed |
description | OBJECTS: To discuss the influence of PM2.5 on myocardial fibrosis and related mechanism. METHODS: PM2.5 particles were prepared into different concentrations of solution to drip into the mice’s trachea twice each week. The mice were divided into five groups, Blank control group (C group), NS control group (J group), high dose group (G group, 10 mg/kg), medium dose group (Z group, 5 mg/kg), and 1ow dose group (D group, 2.5 mg/kg). After 6 weeks, the myocardial fibrosis was observed by HE and Masson staining. The expression of Ang II, ERK1/2, and TGF-β(1) was examined by Western Blotting (WB) and Real time PCR (RT-PCR). RESULTS: The higher dose PM2.5 was administrated, the worse the myocardial fibrosis was in PM2.5 groups. The expression of Ang II, ERK1/2, and TGF-β(1) was increased in higher dose groups in protein and mRNA level. CONCLUSION: 1. PM2.5 induced the cardiac fibrosis. 2. PM2.5 dripped into trachea in mice model activated the expression of Ang II, ERK1/2, and TGF-β(1). The activation of renin-angiotensin system (RAS) was suggested to participate in the cardiac fibrosis induced by PM2.5. |
format | Online Article Text |
id | pubmed-7983242 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-79832422021-03-31 PM2.5 inducing myocardial fibrosis mediated by Ang II/ERK1/2/TGF-β(1) signaling pathway in mice model Zang, Xiwen Zhao, Jun Lu, Chengzhi J Renin Angiotensin Aldosterone Syst Original Research Article OBJECTS: To discuss the influence of PM2.5 on myocardial fibrosis and related mechanism. METHODS: PM2.5 particles were prepared into different concentrations of solution to drip into the mice’s trachea twice each week. The mice were divided into five groups, Blank control group (C group), NS control group (J group), high dose group (G group, 10 mg/kg), medium dose group (Z group, 5 mg/kg), and 1ow dose group (D group, 2.5 mg/kg). After 6 weeks, the myocardial fibrosis was observed by HE and Masson staining. The expression of Ang II, ERK1/2, and TGF-β(1) was examined by Western Blotting (WB) and Real time PCR (RT-PCR). RESULTS: The higher dose PM2.5 was administrated, the worse the myocardial fibrosis was in PM2.5 groups. The expression of Ang II, ERK1/2, and TGF-β(1) was increased in higher dose groups in protein and mRNA level. CONCLUSION: 1. PM2.5 induced the cardiac fibrosis. 2. PM2.5 dripped into trachea in mice model activated the expression of Ang II, ERK1/2, and TGF-β(1). The activation of renin-angiotensin system (RAS) was suggested to participate in the cardiac fibrosis induced by PM2.5. SAGE Publications 2021-03-16 /pmc/articles/PMC7983242/ /pubmed/33726569 http://dx.doi.org/10.1177/14703203211003786 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Article Zang, Xiwen Zhao, Jun Lu, Chengzhi PM2.5 inducing myocardial fibrosis mediated by Ang II/ERK1/2/TGF-β(1) signaling pathway in mice model |
title | PM2.5 inducing myocardial fibrosis mediated by Ang
II/ERK1/2/TGF-β(1) signaling pathway in mice model |
title_full | PM2.5 inducing myocardial fibrosis mediated by Ang
II/ERK1/2/TGF-β(1) signaling pathway in mice model |
title_fullStr | PM2.5 inducing myocardial fibrosis mediated by Ang
II/ERK1/2/TGF-β(1) signaling pathway in mice model |
title_full_unstemmed | PM2.5 inducing myocardial fibrosis mediated by Ang
II/ERK1/2/TGF-β(1) signaling pathway in mice model |
title_short | PM2.5 inducing myocardial fibrosis mediated by Ang
II/ERK1/2/TGF-β(1) signaling pathway in mice model |
title_sort | pm2.5 inducing myocardial fibrosis mediated by ang
ii/erk1/2/tgf-β(1) signaling pathway in mice model |
topic | Original Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983242/ https://www.ncbi.nlm.nih.gov/pubmed/33726569 http://dx.doi.org/10.1177/14703203211003786 |
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