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Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR

Edible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic informati...

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Autores principales: Lv, Dongyong, Fan, Yaohua, Zhong, Wanhua, Lonan, Piyanuch, Liu, Kunfeng, Wu, Maoyong, Wu, Yina, Liang, Yueliang, Lai, Xiaoping, Li, Geng, Yu, Liangwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983251/
https://www.ncbi.nlm.nih.gov/pubmed/33763113
http://dx.doi.org/10.3389/fgene.2021.632232
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author Lv, Dongyong
Fan, Yaohua
Zhong, Wanhua
Lonan, Piyanuch
Liu, Kunfeng
Wu, Maoyong
Wu, Yina
Liang, Yueliang
Lai, Xiaoping
Li, Geng
Yu, Liangwen
author_facet Lv, Dongyong
Fan, Yaohua
Zhong, Wanhua
Lonan, Piyanuch
Liu, Kunfeng
Wu, Maoyong
Wu, Yina
Liang, Yueliang
Lai, Xiaoping
Li, Geng
Yu, Liangwen
author_sort Lv, Dongyong
collection PubMed
description Edible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic information of EBN, a key part of molecular biology, has yet to be reported in Thailand. It is necessary to explore the genetic information of EBN in Thailand based on a quick and simple method to help protect the rights and interests of consumers. This research aimed to systematically evaluate different methods of extracting EBN DNA to improve the efficiency of the analysis of cytochrome b (Cytb) and NADH dehydrogenase subunit 2 (ND2) gene sequences, the establishment of phylogenetic trees, and the genetic information of EBN in Thailand. Additionally, we aimed to develop a quick and simple method for identifying EBN from different species based on the genetic information and amplification-refractory mutation system PCR (ARMS-PCR). By comparing the four methods [cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), kit and guanidinium isothiocyanate methods] for EBN extraction, we found that the guanidinium isothiocyanate method was the optimal extraction method. Phylogenetic trees generated on the basis of Cytb and ND2 gene analyses showed that 26 samples of house EBN and 4 samples of cave EBN came from Aerodramus fuciphagus and Aerodramus maximus, respectively. In addition, to distinguish different samples from different species of Apodiformes, we designed 4 polymerase chain reaction (PCR) amplification primers based on the ND2 gene sequences of A. fuciphagus and A. maximus. The ARMS-PCR results showed band lengths for A. fuciphagus EBN of 533, 402, and 201 bp, while those for A. maximus EBN were 463, 317, and 201 bp. Collectively, the results showed that ARMS-PCR is a fast and simple method for the genetic identification of EBN based on designing specific original identification primers.
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spelling pubmed-79832512021-03-23 Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR Lv, Dongyong Fan, Yaohua Zhong, Wanhua Lonan, Piyanuch Liu, Kunfeng Wu, Maoyong Wu, Yina Liang, Yueliang Lai, Xiaoping Li, Geng Yu, Liangwen Front Genet Genetics Edible bird’s nest (EBN) is a popular delicacy in the Asian Pacific region originating from Indonesia, Malaysia, Thailand and Vietnam, which consist of various potential medicine value in Traditional Chinese Medicine (TCM). Thailand is one of the main exporters of EBN. However, the genetic information of EBN, a key part of molecular biology, has yet to be reported in Thailand. It is necessary to explore the genetic information of EBN in Thailand based on a quick and simple method to help protect the rights and interests of consumers. This research aimed to systematically evaluate different methods of extracting EBN DNA to improve the efficiency of the analysis of cytochrome b (Cytb) and NADH dehydrogenase subunit 2 (ND2) gene sequences, the establishment of phylogenetic trees, and the genetic information of EBN in Thailand. Additionally, we aimed to develop a quick and simple method for identifying EBN from different species based on the genetic information and amplification-refractory mutation system PCR (ARMS-PCR). By comparing the four methods [cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS), kit and guanidinium isothiocyanate methods] for EBN extraction, we found that the guanidinium isothiocyanate method was the optimal extraction method. Phylogenetic trees generated on the basis of Cytb and ND2 gene analyses showed that 26 samples of house EBN and 4 samples of cave EBN came from Aerodramus fuciphagus and Aerodramus maximus, respectively. In addition, to distinguish different samples from different species of Apodiformes, we designed 4 polymerase chain reaction (PCR) amplification primers based on the ND2 gene sequences of A. fuciphagus and A. maximus. The ARMS-PCR results showed band lengths for A. fuciphagus EBN of 533, 402, and 201 bp, while those for A. maximus EBN were 463, 317, and 201 bp. Collectively, the results showed that ARMS-PCR is a fast and simple method for the genetic identification of EBN based on designing specific original identification primers. Frontiers Media S.A. 2021-03-08 /pmc/articles/PMC7983251/ /pubmed/33763113 http://dx.doi.org/10.3389/fgene.2021.632232 Text en Copyright © 2021 Lv, Fan, Zhong, Lonan, Liu, Wu, Wu, Liang, Lai, Li and Yu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Lv, Dongyong
Fan, Yaohua
Zhong, Wanhua
Lonan, Piyanuch
Liu, Kunfeng
Wu, Maoyong
Wu, Yina
Liang, Yueliang
Lai, Xiaoping
Li, Geng
Yu, Liangwen
Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR
title Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR
title_full Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR
title_fullStr Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR
title_full_unstemmed Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR
title_short Genetic Identification of Edible Bird’s Nest in Thailand Based on ARMS-PCR
title_sort genetic identification of edible bird’s nest in thailand based on arms-pcr
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7983251/
https://www.ncbi.nlm.nih.gov/pubmed/33763113
http://dx.doi.org/10.3389/fgene.2021.632232
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