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A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus

Ensuring faithful homologous recombination in allopolyploids is essential to maintain optimal fertility of the species. Variation in the ability to control aberrant pairing between homoeologous chromosomes in Brassica napus has been identified. The current study exploited the extremes of such variat...

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Autores principales: Higgins, Erin E., Howell, Elaine C., Armstrong, Susan J., Parkin, Isobel A. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984352/
https://www.ncbi.nlm.nih.gov/pubmed/33020949
http://dx.doi.org/10.1111/nph.16986
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author Higgins, Erin E.
Howell, Elaine C.
Armstrong, Susan J.
Parkin, Isobel A. P.
author_facet Higgins, Erin E.
Howell, Elaine C.
Armstrong, Susan J.
Parkin, Isobel A. P.
author_sort Higgins, Erin E.
collection PubMed
description Ensuring faithful homologous recombination in allopolyploids is essential to maintain optimal fertility of the species. Variation in the ability to control aberrant pairing between homoeologous chromosomes in Brassica napus has been identified. The current study exploited the extremes of such variation to identify genetic factors that differentiate newly resynthesised B. napus, which is inherently unstable, and established B. napus, which has adapted to largely control homoeologous recombination. A segregating B. napus mapping population was analysed utilising both cytogenetic observations and high‐throughput genotyping to quantify the levels of homoeologous recombination. Three quantitative trait loci (QTL) were identified that contributed to the control of homoeologous recombination in the important oilseed crop B. napus. One major QTL on BnaA9 contributed between 32 and 58% of the observed variation. This study is the first to assess homoeologous recombination and map associated QTLs resulting from deviations in normal pairing in allotetraploid B. napus. The identified QTL regions suggest candidate meiotic genes that could be manipulated in order to control this important trait and further allow the development of molecular markers to utilise this trait to exploit homoeologous recombination in a crop.
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spelling pubmed-79843522021-03-24 A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus Higgins, Erin E. Howell, Elaine C. Armstrong, Susan J. Parkin, Isobel A. P. New Phytol Research Ensuring faithful homologous recombination in allopolyploids is essential to maintain optimal fertility of the species. Variation in the ability to control aberrant pairing between homoeologous chromosomes in Brassica napus has been identified. The current study exploited the extremes of such variation to identify genetic factors that differentiate newly resynthesised B. napus, which is inherently unstable, and established B. napus, which has adapted to largely control homoeologous recombination. A segregating B. napus mapping population was analysed utilising both cytogenetic observations and high‐throughput genotyping to quantify the levels of homoeologous recombination. Three quantitative trait loci (QTL) were identified that contributed to the control of homoeologous recombination in the important oilseed crop B. napus. One major QTL on BnaA9 contributed between 32 and 58% of the observed variation. This study is the first to assess homoeologous recombination and map associated QTLs resulting from deviations in normal pairing in allotetraploid B. napus. The identified QTL regions suggest candidate meiotic genes that could be manipulated in order to control this important trait and further allow the development of molecular markers to utilise this trait to exploit homoeologous recombination in a crop. John Wiley and Sons Inc. 2020-11-03 2021-03 /pmc/articles/PMC7984352/ /pubmed/33020949 http://dx.doi.org/10.1111/nph.16986 Text en © 2020 Her Majesty the Queen in Right of Canada. New Phytologist © 2020 New Phytologist Foundation This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Research
Higgins, Erin E.
Howell, Elaine C.
Armstrong, Susan J.
Parkin, Isobel A. P.
A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus
title A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus
title_full A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus
title_fullStr A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus
title_full_unstemmed A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus
title_short A major quantitative trait locus on chromosome A9, BnaPh1, controls homoeologous recombination in Brassica napus
title_sort major quantitative trait locus on chromosome a9, bnaph1, controls homoeologous recombination in brassica napus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984352/
https://www.ncbi.nlm.nih.gov/pubmed/33020949
http://dx.doi.org/10.1111/nph.16986
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