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A guide to measuring phagosomal dynamics
Phagocytosis is an essential mechanism for immunity and homeostasis, performed by a subset of cells known as phagocytes. Upon target engulfment, de novo formation of specialized compartments termed phagosomes takes place. Phagosomes then undergo a series of fusion and fission events as they interact...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984381/ https://www.ncbi.nlm.nih.gov/pubmed/32757358 http://dx.doi.org/10.1111/febs.15506 |
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author | Levin‐Konigsberg, Roni Mantegazza, Adriana R. |
author_facet | Levin‐Konigsberg, Roni Mantegazza, Adriana R. |
author_sort | Levin‐Konigsberg, Roni |
collection | PubMed |
description | Phagocytosis is an essential mechanism for immunity and homeostasis, performed by a subset of cells known as phagocytes. Upon target engulfment, de novo formation of specialized compartments termed phagosomes takes place. Phagosomes then undergo a series of fusion and fission events as they interact with the endolysosomal system and other organelles, in a dynamic process known as phagosome maturation. Because phagocytes play a key role in tissue patrolling and immune surveillance, phagosome maturation is associated with signaling pathways that link phagocytosis to antigen presentation and the development of adaptive immune responses. In addition, and depending on the nature of the cargo, phagosome integrity may be compromised, triggering additional cellular mechanisms including inflammation and autophagy. Upon completion of maturation, phagosomes enter a recently described phase: phagosome resolution, where catabolites from degraded cargo are metabolized, phagosomes are resorbed, and vesicles of phagosomal origin are recycled. Finally, phagocytes return to homeostasis and become ready for a new round of phagocytosis. Altogether, phagosome maturation and resolution encompass a series of dynamic events and organelle crosstalk that can be measured by biochemical, imaging, photoluminescence, cytometric, and immune‐based assays that will be described in this guide. |
format | Online Article Text |
id | pubmed-7984381 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-79843812021-03-25 A guide to measuring phagosomal dynamics Levin‐Konigsberg, Roni Mantegazza, Adriana R. FEBS J A Guide To… Phagocytosis is an essential mechanism for immunity and homeostasis, performed by a subset of cells known as phagocytes. Upon target engulfment, de novo formation of specialized compartments termed phagosomes takes place. Phagosomes then undergo a series of fusion and fission events as they interact with the endolysosomal system and other organelles, in a dynamic process known as phagosome maturation. Because phagocytes play a key role in tissue patrolling and immune surveillance, phagosome maturation is associated with signaling pathways that link phagocytosis to antigen presentation and the development of adaptive immune responses. In addition, and depending on the nature of the cargo, phagosome integrity may be compromised, triggering additional cellular mechanisms including inflammation and autophagy. Upon completion of maturation, phagosomes enter a recently described phase: phagosome resolution, where catabolites from degraded cargo are metabolized, phagosomes are resorbed, and vesicles of phagosomal origin are recycled. Finally, phagocytes return to homeostasis and become ready for a new round of phagocytosis. Altogether, phagosome maturation and resolution encompass a series of dynamic events and organelle crosstalk that can be measured by biochemical, imaging, photoluminescence, cytometric, and immune‐based assays that will be described in this guide. John Wiley and Sons Inc. 2020-08-16 2021-03 /pmc/articles/PMC7984381/ /pubmed/32757358 http://dx.doi.org/10.1111/febs.15506 Text en © 2020 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | A Guide To… Levin‐Konigsberg, Roni Mantegazza, Adriana R. A guide to measuring phagosomal dynamics |
title | A guide to measuring phagosomal dynamics |
title_full | A guide to measuring phagosomal dynamics |
title_fullStr | A guide to measuring phagosomal dynamics |
title_full_unstemmed | A guide to measuring phagosomal dynamics |
title_short | A guide to measuring phagosomal dynamics |
title_sort | guide to measuring phagosomal dynamics |
topic | A Guide To… |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7984381/ https://www.ncbi.nlm.nih.gov/pubmed/32757358 http://dx.doi.org/10.1111/febs.15506 |
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